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Wang, Zhao, Liu, Yang, Fan, Chen, Cao, Xueli. (1400). One-Step Preparative Separation of Flavone and Isoflavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model. سامانه مدیریت نشریات علمی, 6(3), 148-154. doi: 10.30476/ijns.2021.92070.1146
Zhao Wang; Yang Liu; Chen Fan; Xueli Cao. "One-Step Preparative Separation of Flavone and Isoflavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model". سامانه مدیریت نشریات علمی, 6, 3, 1400, 148-154. doi: 10.30476/ijns.2021.92070.1146
Wang, Zhao, Liu, Yang, Fan, Chen, Cao, Xueli. (1400). 'One-Step Preparative Separation of Flavone and Isoflavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model', سامانه مدیریت نشریات علمی, 6(3), pp. 148-154. doi: 10.30476/ijns.2021.92070.1146
Wang, Zhao, Liu, Yang, Fan, Chen, Cao, Xueli. One-Step Preparative Separation of Flavone and Isoflavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model. سامانه مدیریت نشریات علمی, 1400; 6(3): 148-154. doi: 10.30476/ijns.2021.92070.1146

One-Step Preparative Separation of Flavone and Isoflavone Glycosides from Sophora Japonica Fruit by High-Speed Counter-Current Chromatography Based on COSMO-RS Model

International Journal of Nutrition Sciences
مقاله 7، دوره 6، شماره 3، آذر 2021، صفحه 148-154    اصل مقاله (1.5 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/ijns.2021.92070.1146
نویسندگان
Zhao Wang؛ Yang Liu؛ Chen Fan* ؛ Xueli Cao
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
چکیده
Background: Sophora japonica L. (Leguminosae), a well-known traditional medicine in Asia, is offcially listed in the Chinese and European Pharmacopoeia. Its buds and fruits have been used as medicinal agents with signifcant biological activity. Pharmacologic studies and clinical practice have demonstrated that they have benefcial effects in the treatment of many diseases. Herein, we developed a simpler and effective separation method of flavones and isoflavones from S. japonica fruit by one-step HSCCC based on the COSMO-RS model.
Methods: HSCCC was applied for the preparative separation and purifcation of fve active compounds from S. japonica L. fruit (three flavone glycosides and two isoflavone glycosides). Under the assistance of a COSMO-RS, one-step separation with the two-elution solvent system that was designed.
Results: The frst elution was conducted with the lower phase of ethyl acetate-n-butanol-water (5:1:5, v/v), and the second elution was performed with the same one containing 4.0 mmol L-1 of NH3·H2O. From 200 mg of crude extract, 11.2 mg of kaempferol-3-O sophoroside (I), 3.2 mg of rutin (II), 3.4 mg of kaempferol-3-O-α-L-ramnopyranosyl-(1–6)-β-Dglucopyranoside (III), 11.5 mg of sophorabioside (IV), and 10.1 mg of sophoricoside (V) were obtained. Their purity values were 98.1%, 97.6%, 99.5%, 99.2%, and 98.3%, respectively.
Conclusion: The fve targets of I-V can be used as reference substances for chromatographic purposes as well as for the further physiological studies. The developed method is instructive for the separation of other flavonoids too.

کلیدواژه‌ها
Flavonoid؛ Glycosides؛ Sophora japonica؛ Counter-current chromatography
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