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Sabokrouh, Abdolreza, Ghaffari, Nasim, Hajivand, Soheila, Atabi, Fereshteh, Saghiri, Reza, Sharifi, Zohreh. (1404). Comparison Anticancer Effects of Platinum Ribavirin with Platinum Azidothymidine via Telomerase, Bcl-2, miRNA-21 and miRNA-122 Biomarkers Genes Expression on HepG2 Cancer Cell Lines. سامانه مدیریت نشریات علمی, 16(3), 197-209. doi: 10.30476/mejc.2025.104451.2183
Abdolreza Sabokrouh; Nasim Ghaffari; Soheila Hajivand; Fereshteh Atabi; Reza Saghiri; Zohreh Sharifi. "Comparison Anticancer Effects of Platinum Ribavirin with Platinum Azidothymidine via Telomerase, Bcl-2, miRNA-21 and miRNA-122 Biomarkers Genes Expression on HepG2 Cancer Cell Lines". سامانه مدیریت نشریات علمی, 16, 3, 1404, 197-209. doi: 10.30476/mejc.2025.104451.2183
Sabokrouh, Abdolreza, Ghaffari, Nasim, Hajivand, Soheila, Atabi, Fereshteh, Saghiri, Reza, Sharifi, Zohreh. (1404). 'Comparison Anticancer Effects of Platinum Ribavirin with Platinum Azidothymidine via Telomerase, Bcl-2, miRNA-21 and miRNA-122 Biomarkers Genes Expression on HepG2 Cancer Cell Lines', سامانه مدیریت نشریات علمی, 16(3), pp. 197-209. doi: 10.30476/mejc.2025.104451.2183
Sabokrouh, Abdolreza, Ghaffari, Nasim, Hajivand, Soheila, Atabi, Fereshteh, Saghiri, Reza, Sharifi, Zohreh. Comparison Anticancer Effects of Platinum Ribavirin with Platinum Azidothymidine via Telomerase, Bcl-2, miRNA-21 and miRNA-122 Biomarkers Genes Expression on HepG2 Cancer Cell Lines. سامانه مدیریت نشریات علمی, 1404; 16(3): 197-209. doi: 10.30476/mejc.2025.104451.2183

Comparison Anticancer Effects of Platinum Ribavirin with Platinum Azidothymidine via Telomerase, Bcl-2, miRNA-21 and miRNA-122 Biomarkers Genes Expression on HepG2 Cancer Cell Lines

Middle East Journal of Cancer
مقاله 1، دوره 16، شماره 3، مهر 2025، صفحه 197-209    اصل مقاله (3.14 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2025.104451.2183
نویسندگان
Abdolreza Sabokrouh* 1؛ Nasim Ghaffari2؛ Soheila Hajivand2؛ Fereshteh Atabi2؛ Reza Saghiri3؛ Zohreh Sharifi4
1Department of Biochemistry, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3Department of Biochemistry, Pasteur institute, Tehran, Iran
4Blood Transfusion Research center, High institute for Research and Education in Transfusion Medicine, Tehran, Iran
چکیده
Background: Anticancer medication assessment is performed by numerous approaches including biomarkers gene expression. This study aimed to investigate the anticancer effects of Pt-Rb and Pt-AZTon HepG2 cells. 
Method: In this case-control study, four groups of cells were examined. Group A was the control group, group B was untreated cancer cells, and Groups C and D were treated with Pt-AZT and Pt-Rb, respectively. Using the MTT test, LC50 was determined, and the relative gene expression of the biomarkers was assessed by RNA extraction, cDNA synthesis and RT-PCR. Through histopathlogical study apoptotic regions of cells were compared. Data analysis was done using ANOVA and Turkey’s post hoc test.
Results: The results showed a significant increase for the proapoptotic gene miRNA-122 (19.97 ± 0 .04) in group D compared with group C (10.36 ± 0.007). Also, there was a significant decrease in the antiapoptotic genes in group D, including miRNA-21 (0.10 ± 0.014), telomerase (0.56 ± 0.480), and Bcl-2 (0.41 ± 0.276), compared with group C (miRNA-21: 2.0 ± 0.145, telomerase: 2.49 ± 0.231, and Bcl-2: 2.93 ± 0.276). There were significant differences between the nearly all studied groups (P < 0.05). There were more extensive apoptotic regions in group D compared with group C.
Conclusion: Using Pt-Rb has more benefits in terms of stronger anticancer effects than Pt-AZT on cancer cells. Also, lower drug resistance and lower side effects in Pt-Rb were considered compared with Pt-AZT, indicating that it can be more effective in anti-cancer therapy.

تازه های تحقیق

Abdolreza Sabokrouh (Google Scholar)

کلیدواژه‌ها
miRNAs؛ Platinum compounds؛ HepG2 cells؛ Telomerase
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