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Davari Zanjani, Maryam, Khodabandeh, Zahra, Edalatmanesh, Mohammad Amin. (1404). The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide. سامانه مدیریت نشریات علمی, 50(4), 260-269. doi: 10.30476/ijms.2024.101801.3444
Maryam Davari Zanjani; Zahra Khodabandeh; Mohammad Amin Edalatmanesh. "The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide". سامانه مدیریت نشریات علمی, 50, 4, 1404, 260-269. doi: 10.30476/ijms.2024.101801.3444
Davari Zanjani, Maryam, Khodabandeh, Zahra, Edalatmanesh, Mohammad Amin. (1404). 'The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide', سامانه مدیریت نشریات علمی, 50(4), pp. 260-269. doi: 10.30476/ijms.2024.101801.3444
Davari Zanjani, Maryam, Khodabandeh, Zahra, Edalatmanesh, Mohammad Amin. The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide. سامانه مدیریت نشریات علمی, 1404; 50(4): 260-269. doi: 10.30476/ijms.2024.101801.3444

The Protective Effect of Taurine and Curcumin on Autophagy-Related Genes in the Oocytes of the Mouse Treated with Acrylamide

Iranian Journal of Medical Sciences
مقاله 6، دوره 50، شماره 4، تیر 2025، صفحه 260-269    اصل مقاله (563.09 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2024.101801.3444
نویسندگان
Maryam Davari Zanjani1؛ Zahra Khodabandeh* 2؛ Mohammad Amin Edalatmanesh1
1Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Autophagy is also essential for both male and female infertility since it controls the development of germ cells and reproductive organs. This study aimed to investigate the effects of taurine and curcumin on the expression of genes related to autophagy in acrylamide-treated mice. 
Methods: In 2022, this experimental study was conducted at the Shiraz University of Medical Sciences, Stem Cells Technology Research Centre. Forty-eight mice were randomly assigned to eight groups (control, curcumin 200 mg/Kg, taurine 150 mg/Kg, acrylamide 50 mg/Kg, acrylamide+curcumin 100 mg/Kg, acrylamide+curcumin 200, acrylamide+taurine 75 mg/Kg, acrylamide+taurine 150 mg/Kg). Finally, oocyte characteristics and gene expression were determined in each group using one-way analysis of variance (ANOVA) by SPSS 25 and GraphPad 9, respectively. P<0.05 was conducted statistically significant. 
Results: A significant decrease was observed in several oocytes in the acrylamide group compared to the control group (P<0.001). The expression levels of light chain 3 (LC3), autophagy-related gene (ATG)12, ATG5, and Beclin1 significantly increased in the acrylamide compared to the control group. A significant increase in the number of oocytes was observed in the taurine group compared to the control. The expression levels of LC3, ATG12, ATG5, and Beclin1 significantly decreased in the acrylamide+taurine (150 mg/Kg) compared to the acrylamide group. 
Conclusion: The acrylamide negatively impacts oocyte viability and causes the higher expression of autophagy-related genes. Taurine may encourage the fusion of autophagosomes with lysosomes by removing autophagic obstruction, potentially accelerating autophagy and protecting against oxidative stress. Taurine is more effective than curcumin at reducing the harmful effects of acrylamide. As a result, taurine can be proposed as a potential treatment drug for acrylamide-induced infertility.
کلیدواژه‌ها
Acrylamide؛ Oocyte؛ Mitochondria؛ Autophagy؛ Taurin؛ Curcumin
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مراجع
  1. Muttucumaru N, Powers SJ, Elmore JS, Dodson A, Briddon A, Mottram DS, et al. Acrylamide-forming potential of potatoes grown at different locations, and the ratio of free asparagine to reducing sugars at which free asparagine becomes a limiting factor for acrylamide formation. Food Chem. 2017;220:76-86. doi: 10.1016/j.foodchem.2016.09.199. PubMed PMID: 27855938; PubMed Central PMCID: PMCPMC5119237.
  2. Nan B, Yang C, Li L, Ye H, Yan H, Wang M, et al. Allicin alleviated acrylamide-induced NLRP3 inflammasome activation via oxidative stress and endoplasmic reticulum stress in Kupffer cells and SD rats liver. Food Chem Toxicol. 2021;148:111937. doi: 10.1016/j.fct.2020.111937. PubMed PMID: 33348049.
  3. Aldawood N, Alrezaki A, Alanazi S, Amor N, Alwasel S, Sirotkin A, et al. Acrylamide impairs ovarian function by promoting apoptosis and affecting reproductive hormone release, steroidogenesis and autophagy-related genes: An in vivo study. Ecotoxicol Environ Saf. 2020;197:110595. doi: 10.1016/j.ecoenv.2020.110595. PubMed PMID: 32304918.
  4. Zamani E, Shokrzadeh M, Fallah M, Shaki F. A review of acrylamide toxicity and its mechanism. Pharmaceutical and biomedical research. 2017;3:1-7. doi: 10.18869/acadpub.pbr.3.1.1.
  5. Ommati MM, Heidari R, Manthari RK, Tikka Chiranjeevi S, Niu R, Sun Z, et al. Paternal exposure to arsenic resulted in oxidative stress, autophagy, and mitochondrial impairments in the HPG axis of pubertal male offspring. Chemosphere. 2019;236:124325. doi: 10.1016/j.chemosphere.2019.07.056. PubMed PMID: 31326754.
  6. Wang L, Ye X, Zhao T. The physiological roles of autophagy in the mammalian life cycle. Biol Rev Camb Philos Soc. 2019;94:503-16. doi: 10.1111/brv.12464. PubMed PMID: 30239126; PubMed Central PMCID: PMCPMC7379196.
  7. Cao B, Camden AJ, Parnell LA, Mysorekar IU. Autophagy regulation of physiological and pathological processes in the female reproductive tract. Am J Reprod Immunol. 2017;77. doi: 10.1111/aji.12650. PubMed PMID: 28194822.
  8. Alaee S, Mirani M, Derakhshan Z, Koohpeyma F, Bakhtari A. Thymoquinone improves folliculogenesis, sexual hormones, gene expression of apoptotic markers and antioxidant enzymes in polycystic ovary syndrome rat model. Vet Med Sci. 2023;9:290-300. doi: 10.1002/vms3.958. PubMed PMID: 36104839; PubMed Central PMCID: PMCPMC9857009.
  9. Ojatula AO. Developmental botanic: A case study on chromatographic determination of phytocompounds in the roots of anthocleista nobilis (G. Don.) as pro-fertility. Journal of Infertility and Reproductive Biology. 2021;9:7-21.
  10. Alaee S, Khodabandeh Z, Dara M, Hosseini E, Sharma M. Curcumin mitigates acrylamide-induced ovarian antioxidant disruption and apoptosis in female Balb/c mice: A comprehensive study on gene and protein expressions. Food Sci Nutr. 2024;12:4160-72. doi: 10.1002/fsn3.4076. PubMed PMID: 38873462; PubMed Central PMCID: PMCPMC11167175.
  11. Schaffer S, Kim HW. Effects and Mechanisms of Taurine as a Therapeutic Agent. Biomol Ther (Seoul). 2018;26:225-41. doi: 10.4062/biomolther.2017.251. PubMed PMID: 29631391; PubMed Central PMCID: PMCPMC5933890.
  12. De Luca A, Pierno S, Camerino DC. Taurine: the appeal of a safe amino acid for skeletal muscle disorders. J Transl Med. 2015;13:243. doi: 10.1186/s12967-015-0610-1. PubMed PMID: 26208967; PubMed Central PMCID: PMCPMC4513970.
  13. Baliou S, Adamaki M, Ioannou P, Pappa A, Panayiotidis MI, Spandidos DA, et al. Protective role of taurine against oxidative stress (Review). Mol Med Rep. 2021;24. doi: 10.3892/mmr.2021.12242. PubMed PMID: 34184084; PubMed Central PMCID: PMCPMC8240184.
  14. Zhang Z, Liu D, Yi B, Liao Z, Tang L, Yin D, et al. Taurine supplementation reduces oxidative stress and protects the liver in an iron-overload murine model. Mol Med Rep. 2014;10:2255-62. doi: 10.3892/mmr.2014.2544. PubMed PMID: 25201602; PubMed Central PMCID: PMCPMC4199407.
  15. Mousavi K, Niknahad H, Ghalamfarsa A, Mohammadi H, Azarpira N, Ommati MM, et al. Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms. Clin Exp Hepatol. 2020;6:207-19. doi: 10.5114/ceh.2020.99513. PubMed PMID: 33145427; PubMed Central PMCID: PMCPMC7592093.
  16. Azami SH, Nazarian H, Abdollahifar MA, Eini F, Farsani MA, Novin MG. The antioxidant curcumin postpones ovarian aging in young and middle-aged mice. Reprod Fertil Dev. 2020;32:292-303. doi: 10.1071/RD18472. PubMed PMID: 31656219.
  17. Golmohammadi T, Vaezi G. Effect of Curcuma Longa on In Vitro Maturation of Immature Oocytes and Embryonic Development in NMRI Mice. Journal of Mazandaran University of Medical Sciences. 2018;27:49-58.
  18. Huynh DTM, De Tran V, Pan-On S, Pham DT. In-vivo anti-tumor activities of curcumin-solid-dispersion loaded floating tablets on stomach and lung cancers. Journal of Applied Pharmaceutical Science. 2024;14:081-7.
  19. Chen J, Li J, Fan T, Zhong S, Qin X, Li R, et al. Protective effects of curcumin/cyclodextrin polymer inclusion complex against hydrogen peroxide-induced LO2 cells damage. Food Sci Nutr. 2022;10:1649-56. doi: 10.1002/fsn3.2787. PubMed PMID: 35592280; PubMed Central PMCID: PMCPMC9094476.
  20. Care IoLARCo, Animals UoL. Guide for the Care and Use of Laboratory Animals. The National Academies Collection: Reports funded by National Institutes of Health. 8th ed. Institute of Laboratory Animal Resources: Washington (DC); 2011.
  21. Daneshpazhouh H, Hayati Roodbari N, Tahamtani Y, Khodabandeh Z, Dianatpour M. Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes. Iran J Med Sci. 2023;48:501-9. doi: 10.30476/IJMS.2023.88390.2811. PubMed PMID: 37786462; PubMed Central PMCID: PMCPMC10541544.
  22. Roy D, Tomo S, Modi A, Purohit P, Sharma P. Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study. MethodsX. 2020;7:101113. doi: 10.1016/j.mex.2020.101113. PubMed PMID: 33204654; PubMed Central PMCID: PMCPMC7653057.
  23. Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2^(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2013;3:71-85. PubMed PMID: 25558171; PubMed Central PMCID: PMCPMC4280562.
  24. Ommati MM, Heidari R, Jamshidzadeh A, Zamiri MJ, Sun Z, Sabouri S, et al. Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models. Toxicol Lett. 2018;284:46-55. doi: 10.1016/j.toxlet.2017.11.034. PubMed PMID: 29197623.
  25. Li S, Zhao H, Wang Y, Shao Y, Wang B, Wang Y, et al. Regulation of autophagy factors by oxidative stress and cardiac enzymes imbalance during arsenic or/and copper induced cardiotoxicity in Gallus gallus. Ecotoxicol Environ Saf. 2018;148:125-34. doi: 10.1016/j.ecoenv.2017.10.018. PubMed PMID: 29035754.
  26. Song D, Xu C, Holck AL, Liu R. Acrylamide inhibits autophagy, induces apoptosis and alters cellular metabolic profiles. Ecotoxicol Environ Saf. 2021;208:111543. doi: 10.1016/j.ecoenv.2020.111543. PubMed PMID: 33396091.
  27. Park H, Cho M, Do Y, Park JK, Bae SJ, Joo J, et al. Autophagy as a Therapeutic Target of Natural Products Enhancing Embryo Implantation. Pharmaceuticals (Basel). 2021;15. doi: 10.3390/ph15010053. PubMed PMID: 35056110; PubMed Central PMCID: PMCPMC8779555.
  28. Zheng Y, Ma L, Liu N, Tang X, Guo S, Zhang B, et al. Autophagy and Apoptosis of Porcine Ovarian Granulosa Cells During Follicular Development. Animals (Basel). 2019;9. doi: 10.3390/ani9121111. PubMed PMID: 31835576; PubMed Central PMCID: PMCPMC6940823.
  29. Saifi B, Haftcheshmeh SM, Feligioni M, Izadpanah E, Rahimi K, Hassanzadeh K, et al. An overview of the therapeutic effects of curcumin in reproductive disorders with a focus on the antiinflammatory and immunomodulatory activities. Phytother Res. 2022;36:808-23. doi: 10.1002/ptr.7360. PubMed PMID: 35041229.
  30. Maiti P, Scott J, Sengupta D, Al-Gharaibeh A, Dunbar GL. Curcumin and Solid Lipid Curcumin Particles Induce Autophagy, but Inhibit Mitophagy and the PI3K-Akt/mTOR Pathway in Cultured Glioblastoma Cells. Int J Mol Sci. 2019;20. doi: 10.3390/ijms20020399. PubMed PMID: 30669284; PubMed Central PMCID: PMCPMC6359162.
  31. Peters AE, Mihalas BP, Bromfield EG, Roman SD, Nixon B, Sutherland JM. Autophagy in Female Fertility: A Role in Oxidative Stress and Aging. Antioxid Redox Signal. 2020;32:550-68. doi: 10.1089/ars.2019.7986. PubMed PMID: 31892284.
  32. Khayatan D, Razavi SM, Arab ZN, Hosseini Y, Niknejad A, Momtaz S, et al. Superoxide dismutase: a key target for the neuroprotective effects of curcumin. Mol Cell Biochem. 2024;479:693-705. doi: 10.1007/s11010-023-04757-5. PubMed PMID: 37166541.
  33. Gao N, Yao X, Jiang L, Yang L, Qiu T, Wang Z, et al. Taurine improves low-level inorganic arsenic-induced insulin resistance by activating PPARgamma-mTORC2 signalling and inhibiting hepatic autophagy. J Cell Physiol. 2019;234:5143-52. doi: 10.1002/jcp.27318. PubMed PMID: 30362509.
  34. Kaneko H, Kobayashi M, Mizunoe Y, Yoshida M, Yasukawa H, Hoshino S, et al. Taurine is an amino acid with the ability to activate autophagy in adipocytes. Amino Acids. 2018;50:527-35. doi: 10.1007/s00726-018-2550-6. PubMed PMID: 29523960.
  35. Liu K, Mao X, Li H, Liu D, Le G, Gan F, et al. Regulation of taurine in OTA-induced apoptosis and autophagy. Toxicon. 2020;181:82-90. doi: 10.1016/j.toxicon.2020.04.097. PubMed PMID: 32371067.
  36. Sun Y, Dai S, Tao J, Li Y, He Z, Liu Q, et al. Taurine suppresses ROS-dependent autophagy via activating Akt/mTOR signaling pathway in calcium oxalate crystals-induced renal tubular epithelial cell injury. Aging (Albany NY). 2020;12:17353-66. doi: 10.18632/aging.103730. PubMed PMID: 32931452; PubMed Central PMCID: PMCPMC7521519.
  37. Tan X, Ye J, Liu W, Zhao B, Shi X, Zhang C, et al. Acrylamide aggravates cognitive deficits at night period via the gut-brain axis by reprogramming the brain circadian clock. Arch Toxicol. 2019;93:467-86. doi: 10.1007/s00204-018-2340-7. PubMed PMID: 30374679.
  38. Schaaf MB, Keulers TG, Vooijs MA, Rouschop KM. LC3/GABARAP family proteins: autophagy-(un)related functions. FASEB J. 2016;30:3961-78. doi: 10.1096/fj.201600698R. PubMed PMID: 27601442.
  39. Yang N, Li L, Li Z, Ni C, Cao Y, Liu T, et al. Protective effect of dapsone on cognitive impairment induced by propofol involves hippocampal autophagy. Neurosci Lett. 2017;649:85-92. doi: 10.1016/j.neulet.2017.04.019. PubMed PMID: 28411068.
  40. Zou H, Wang T, Yuan J, Sun J, Yuan Y, Gu J, et al. Cadmium-induced cytotoxicity in mouse liver cells is associated with the disruption of autophagic flux via inhibiting the fusion of autophagosomes and lysosomes. Toxicol Lett. 2020;321:32-43. doi: 10.1016/j.toxlet.2019.12.019. PubMed PMID: 31862506.
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