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Rezai, Shaghayegh, Ghorbani, Elnaz, Nazari, Seyedeh Elnaz, Rahmani, Farzad, Hassanian, Seyed Mahdi, Afshari, Asma, Habibi Najafi, Mohammad Bagher, Avan, Amir, Ryzhikov, Mikhail, Soleimanpour, Saman, Khazei, Majid. (1403). Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2024.102396.3530
Shaghayegh Rezai; Elnaz Ghorbani; Seyedeh Elnaz Nazari; Farzad Rahmani; Seyed Mahdi Hassanian; Asma Afshari; Mohammad Bagher Habibi Najafi; Amir Avan; Mikhail Ryzhikov; Saman Soleimanpour; Majid Khazei. "Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model". سامانه مدیریت نشریات علمی, , , 1403, -. doi: 10.30476/ijms.2024.102396.3530
Rezai, Shaghayegh, Ghorbani, Elnaz, Nazari, Seyedeh Elnaz, Rahmani, Farzad, Hassanian, Seyed Mahdi, Afshari, Asma, Habibi Najafi, Mohammad Bagher, Avan, Amir, Ryzhikov, Mikhail, Soleimanpour, Saman, Khazei, Majid. (1403). 'Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2024.102396.3530
Rezai, Shaghayegh, Ghorbani, Elnaz, Nazari, Seyedeh Elnaz, Rahmani, Farzad, Hassanian, Seyed Mahdi, Afshari, Asma, Habibi Najafi, Mohammad Bagher, Avan, Amir, Ryzhikov, Mikhail, Soleimanpour, Saman, Khazei, Majid. Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model. سامانه مدیریت نشریات علمی, 1403; (): -. doi: 10.30476/ijms.2024.102396.3530

Investigation of Lactobacillus Probiotics Derived from Traditional Dairy Products in Eliciting Anti-Tumor Responses in Mouse Colorectal Cancer Model

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 10 دی 1403    اصل مقاله (2.17 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2024.102396.3530
نویسندگان
Shaghayegh Rezai* 1؛ Elnaz Ghorbani1؛ Seyedeh Elnaz Nazari2؛ Farzad Rahmani3؛ Seyed Mahdi Hassanian3، 4؛ Asma Afshari5؛ Mohammad Bagher Habibi Najafi6؛ Amir Avan3، 7؛ Mikhail Ryzhikov8؛ Saman Soleimanpour1؛ Majid Khazei2
1Department of Medical Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Medical Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
7Department of Human Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
8Saint Louis University, School of Medicine, Saint Louis, MO, USA
چکیده
Background: Colorectal cancer (CRC) is a serious health problem, and finding new treatments is important. There is growing evidence for the antitumor activity of probiotics. This study investigated the anti-cancer potential of a probiotic mix containing Lactobacillus plantarum, L. brevis, L. helveticus, and L. delbrueckii, alone or in combination with the chemotherapy drug 5-fluorouracil, against CRC. 
Methods: The research was carried out in Mashhad in 2021. The cytotoxic effect of Lactobacillus isolates on CRC cells was investigated in two-dimensional and three-dimensional cell culture models. Histological staining and molecular approaches were used to investigate the regulatory mechanism of Lactobacillus isolates on cell migration, inflammation, fibrosis, cell cycle progression, apoptosis, and tumor necrosis in the CRC mouse model. Statistical analysis was performed using SPSS software version 20 with a significance level of P<0.05. The tests employed included the Kolmogorov–Smirnov, ANOVA, Dunnett’s post hoc, and Kruskal–Wallis.
Results: Lactobacillus strains effectively suppressed tumor growth in CRC by promoting cell death and inhibiting fibrosis and inflammation. These bacteria regulated apoptosis-related genes such as Bcl-2-associated protein x (P=0.0033), and BCL-2 (P=0.0029), leading to increased tumor necrosis. Treatment with bacterial supernatants reduced tumor size and fibrosis by downregulating collagen type I, alpha 1 (Col1a1) (P=0.024), Col1a2 (P=0.0231), and actin alpha 2 (P=0.0466), and transforming growth factor-beta expression. Additionally, they suppressed inflammation by decreasing tumor necrosis factor-alpha (P=0.0001), interleukin 6, and IL-1β (P=0.0198) levels in tumor tissues. Furthermore, the treatment inhibited CRC cell migration by modulating epithelial cadherin (P=0.0198) and matrix metallopeptidase 2 (P=0.033) expression.  
Conclusion: Findings indicated that co-administration of Lactobacillus isolates with 5-FU could improve the anti-tumor properties of the standard drug, 5-FU, supporting the therapeutic potential of these safe isolated lactic acid bacteria for CRC patients in vivo.
کلیدواژه‌ها
Probiotic؛ Lactobacillus؛ Colorectal cancer؛ Inflammation؛ Fibrosis
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