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Evaluation of Metastasis Inhibition by ABD-IL-2 Compared to Human IL-2 in a Breast Cancer Mouse Model | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 4، اسفند 2024، صفحه 294-301 اصل مقاله (1.27 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.103157.2819 | ||
| نویسندگان | ||
| Maryam Teimouri1، 2؛ Ahad Muhammadnejad3؛ Mir Saeed Yekaninejad4؛ Alireza Razavi1؛ Gholam Ali Kardar* 2، 5 | ||
| 1Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 2Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 3Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 4Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. | ||
| 5Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. | ||
| چکیده | ||
| Background: Interleukin-2 (IL-2) is a well-known cytokine that plays a crucial role in stimulating immune cells, including natural killer (NK) cells and cytotoxic T cells. It has been studied as an immunotherapy for a variety of diseases, including cancer. However, due to its short serum half-life, high doses of IL-2 are required which can result in systemic toxicities like capillary leak syndrome. Objective: To demonstrate the enhanced antitumor efficacy of Albumin Binding Domain-conjugated IL-2 (ABD-IL-2) at a lower dose compared to IL-2. Methods: IL-2 and ABD-IL-2 were purified using Ni-NTA resin with a histidine sequence added to their C-terminal region for purification purpose. Peripheral blood lymphocytes were stimulated with IL-2 and ABD-IL-2 to assess their function. 4T1 cells were injected into BALB/c mice to establish a breast cancer model with metastasis evaluated in the lungs. Results: Both recombinant proteins significantly stimulated T lymphocyte proliferation compared to the negative control (P=0.000, P=0.001). Administration of both proteins reduced the size of isolated tumors in the breast cancer mouse model. The control group had more nodules and larger lung metastatic centers (P=0.000). Metastasis to secondary lymphoid organs occurred only in the control group. Conclusion: By using ABD-IL-2 at a one-third concentration compared to IL-2, we aimed to reduce administration toxicity associated with high doses of IL-2 in immunotherapy. This approach shows potential for improving IL-2-based treatments while minimizing adverse effects. | ||
| کلیدواژه‌ها | ||
| Breast Cancer؛ Interleukin-2؛ Metastasis؛ Tumor | ||
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آمار تعداد مشاهده مقاله: 331 تعداد دریافت فایل اصل مقاله: 233 |
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