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Immune Cell Dysregulation in Hindlimb Unloading Mouse Model: Implications for Space Applications | ||
| Journal of Biomedical Physics and Engineering | ||
| مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 13 آذر 1403 اصل مقاله (1.33 M) | ||
| نوع مقاله: Original Research | ||
| نویسندگان | ||
| Sadegh Masoudi1؛ Mehdi Kalani2؛ Ali Alavianmehr3؛ Mohammad Amin Mosleh-Shirazi1، 4؛ Seyed Mohammad Javad Mortazavi* 1، 5؛ Shirin Farjadian* 3 | ||
| 1Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Radiotherapy Department and Center for Research in Medical Physics and Biomedical Engineering, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Department of Medical Physics and Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Hindlimb unloading (HU) mice is a ground-based model that simulates the effects of microgravity. Since microgravity significantly affects the immune system, understanding immune cell function under these conditions is crucial for developing strategies to protect astronauts from infections and malignancies during long space missions. Objective: To evaluate how microgravity affects neutrophils and T cells as the key components of innate and adaptive immunity, the activity of these cells in HU mice was compared with untreated control mice. Material and Methods: In this experimental study, 10 HU male BALB/c mice and 10 untreated control mice were included. Neutrophil-to-lymphocyte ratio (NLR) was evaluated and neutrophil function was assessed using the DHR assay. T cell proliferation was evaluated using the CFSE-dilution assay. IL-4 and IFN-ɣ production by T cell subsets was determined by intracellular cytokine staining with flow cytometry. Results: The capacity for reactive oxygen species (ROS) production in neutrophils did not differ between HU mice and control mice however, NLR was higher in HU mice. The proliferation of both CD4+ and CD8+ T cells was slightly reduced in HU mice. More notably, IL-4 production by CD4+ T cells and IFN-ɣ production by both CD4+ and CD8+ T cells were significantly decreased in HU mice. Conclusion: Hindlimb unloading, simulating microgravity, impairs immune cell functions by reducing cytokine production and T cell proliferation. The increased NLR in HU mice could indicate a heightened inflammatory response. These insights are essential for advancing space biology and medicine, ensuring astronaut health during prolonged space travel. | ||
| کلیدواژه‌ها | ||
| Hindlimb Unloading؛ Immune System؛ Neutrophils؛ T-Lymphocytes؛ Cytokines؛ Microgravity؛ Space؛ Astronauts | ||
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آمار تعداد مشاهده مقاله: 50 تعداد دریافت فایل اصل مقاله: 65 |
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