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Sandra, Ferry, Dewi, Dian Andriani Ratna, Mantik Astawa, I Nyoman, Pangkahila, Wimpie, Chouw, Angliana, Celinna, Maria, Mustika Dewi, Nurrani. (1403). Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/ijms.2024.100856.3342
Ferry Sandra; Dian Andriani Ratna Dewi; I Nyoman Mantik Astawa; Wimpie Pangkahila; Angliana Chouw; Maria Celinna; Nurrani Mustika Dewi. "Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway". سامانه مدیریت نشریات علمی, , , 1403, -. doi: 10.30476/ijms.2024.100856.3342
Sandra, Ferry, Dewi, Dian Andriani Ratna, Mantik Astawa, I Nyoman, Pangkahila, Wimpie, Chouw, Angliana, Celinna, Maria, Mustika Dewi, Nurrani. (1403). 'Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/ijms.2024.100856.3342
Sandra, Ferry, Dewi, Dian Andriani Ratna, Mantik Astawa, I Nyoman, Pangkahila, Wimpie, Chouw, Angliana, Celinna, Maria, Mustika Dewi, Nurrani. Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway. سامانه مدیریت نشریات علمی, 1403; (): -. doi: 10.30476/ijms.2024.100856.3342

Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway

Iranian Journal of Medical Sciences
مقالات آماده انتشار، اصلاح شده برای چاپ، انتشار آنلاین از تاریخ 16 مهر 1403    اصل مقاله (2.22 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2024.100856.3342
نویسندگان
Ferry Sandra* 1؛ Dian Andriani Ratna Dewi2؛ I Nyoman Mantik Astawa3؛ Wimpie Pangkahila4؛ Angliana Chouw5؛ Maria Celinna6؛ Nurrani Mustika Dewi6
1Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia
2Gatot Soebroto Army Hospital, Jakarta, Indonesia
3Faculty of Veterinary Medicine, Universitas Udayana, Denpasar, Indonesia
4Department of Andrology and Sexology, Faculty of Medicine, Universitas Udayana, Denpasar, Indonesia
5PT Prodia StemCell Indonesia, Jakarta, Indonesia
6The Prodia Education and Research Institute, Jakarta, Indonesia
چکیده
Background: Ultraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for cell regeneration. However, the potential of CM-hUCB-MSC in preventing UVB-induced apoptosis has not been clearly elucidated. Therefore, the current research was conducted to investigate the potential of CM-hUCB-MSC in inhibiting UVB-induced apoptosis and its role in the antiapoptotic signaling pathway.
Methods: An experimental in vitro study was conducted at PT. Prodia StemCell Indonesia, Jakarta, Indonesia, 2019-2022. Initially, hUCB-MSCs were isolated and cultured to produce CM-hUCB-MSC. NIH3T3 cells were pretreated with/without 50 μM LY294002, treated with/without 10% CM-hUCB-MSC, and then irradiated with/without UVB. Subsequently, the cells were analyzed using sub-G1, immunofluorescence, and immunoblotting assays. One-way analysis of variance (ANOVA) was used for data analysis, followed by Tukey’s honest significant difference (HSD) test or the Kruskal-Wallis test, followed by the Dunn-Bonferroni test using IBM SPSS Statistics software version 21. Statistical significance was determined at P<0.05.
Results: CM-hUCB-MSC significantly inhibited UVB-induced apoptosis in NIH3T3 cells (P=0.002, Dunn-Bonferroni test). CM-hUCB-MSC significantly induced Akt phosphorylation at Ser 473 in UVB-irradiated NIH3T3 cells (P<0.001, Tukey’s HSD test). The CM-hUCB-MSC-induced phosphorylation of Akt was significantly inhibited by LY294002 (P<0.001, Tukey’s HSD test).
Conclusion: Taken together, it can be concluded that CM-hUCB-MSC inhibits UVB-induced NIH3T3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (PI3K)/Akt signaling cascades.
کلیدواژه‌ها
Ultraviolet rays؛ Apoptosis؛ Culture media, conditioned؛ Mesenchymal stem cells؛ Proto-oncogene proteins c-akt
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