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Zarei, Ali Asghar, Keshtgar, Sara, Haghani, Masoud, Firouzabadi, Negar, Nasr-Esfahani, Mohammad Hossein. (1403). Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function. سامانه مدیریت نشریات علمی, 50(3), 187-196. doi: 10.30476/ijms.2024.102383.3528
Ali Asghar Zarei; Sara Keshtgar; Masoud Haghani; Negar Firouzabadi; Mohammad Hossein Nasr-Esfahani. "Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function". سامانه مدیریت نشریات علمی, 50, 3, 1403, 187-196. doi: 10.30476/ijms.2024.102383.3528
Zarei, Ali Asghar, Keshtgar, Sara, Haghani, Masoud, Firouzabadi, Negar, Nasr-Esfahani, Mohammad Hossein. (1403). 'Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function', سامانه مدیریت نشریات علمی, 50(3), pp. 187-196. doi: 10.30476/ijms.2024.102383.3528
Zarei, Ali Asghar, Keshtgar, Sara, Haghani, Masoud, Firouzabadi, Negar, Nasr-Esfahani, Mohammad Hossein. Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function. سامانه مدیریت نشریات علمی, 1403; 50(3): 187-196. doi: 10.30476/ijms.2024.102383.3528

Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function

Iranian Journal of Medical Sciences
مقاله 6، دوره 50، شماره 3، خرداد 2025، صفحه 187-196    اصل مقاله (1.45 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2024.102383.3528
نویسندگان
Ali Asghar Zarei1؛ Sara Keshtgar* 1؛ Masoud Haghani1؛ Negar Firouzabadi2؛ Mohammad Hossein Nasr-Esfahani3
1Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
چکیده
Background: Calcium enters human sperm through the “Cation Channel of Sperm” (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca2+]i) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function. 
Methods: This study was conducted at the Physiology Department of Shiraz Medical School in 2022. Thirty-six semen samples were washed, and the supernatants were discarded. Then, 0.5 mL of modified-supplemented Earle’s balanced salt solution (M-sEBSS) was added to the sediments. Samples were incubated at 37 ̊C and 5% CO2. The motile spermatozoa were aspirated after swimming up, and the samples were diluted with M-sEBSS to 10×106 spermatozoa per mL. The samples were divided into four groups: control, solvent (dimethyl-sulphoxide (DMSO), NNC55-0396 (NNC) (10 μM), and quinidine (100 μM) as CatSper and KSper blockers, respectively. Sperm kinematics were evaluated by a computer-assisted sperm analyzer. The samples were stained with Eosin-Y and FITC-PSA to assess sperm viability and acrosomal reaction. The [Ca2+]i was examined by flow cytometry using Fluo-3AM. The parametric and non-parametric data were analyzed using one-way ANOVA and Kruskal-Wallis test.
Results: NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca2+]i in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012). 
Conclusion: Inhibition of KSper without effect on [Ca2+]i can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.
کلیدواژه‌ها
Calcium channels؛ Potassium channels؛ NNC 55-0396؛ Quinidine
مراجع
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