پیوندهای مفید
آمار
| تعداد نشریات | 20 |
| تعداد شماره‌ها | 1,149 |
| تعداد مقالات | 10,518 |
| تعداد مشاهده مقاله | 45,415,955 |
| تعداد دریافت فایل اصل مقاله | 11,291,587 |
JAK/STAT Signaling Pathway Mediates Anti-Tumor Immunity of CD8+ T Cells in Renal Cancer | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 3، آذر 2024، صفحه 186-200 اصل مقاله (4.51 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.103692.2852 | ||
| نویسندگان | ||
| Jia Shao1؛ Gang Deng1؛ Guojun Wen2؛ Xi Xie* 1 | ||
| 1Department of Urology, Hangzhou First People’ Hospital, Hangzhou 310002, Zhejiang Province, China. | ||
| 2Department of Urology, Hangzhou First People's Hospital, Lishui 323700, Zhejiang Province, China. | ||
| چکیده | ||
| Background: CD8+ T cells play a crucial role in immune responses, and have significant potential in tumor immunotherapy. The JAK/STAT pathway is essential for cytokine signal transduction and is linked to immune escape. However, its role in mediating CD8+ T cell anti-tumor immunity in renal cancer is not fully understood. Objective: To study the mechanisms underlying CD8+ T cell-mediated anti-tumor immunity and propose new possibilities for immunotherapy in patients with renal cancer. Methods: CD8+ T cells from mouse spleens were sorted using immunomagnetic beads, and their purity was confirmed by flow cytometry. Proliferation was analyzed using CCK-8 and CFSE assays. Activation of CD8+ T cells was assessed through ELISA and Western blotting. The malignant properties of Renca cells were evaluated through flow cytometry, Calcein-AM/PI staining, wound healing, Transwell, Western blot, and immunofluorescence. A subcutaneous tumor model in nude mice was used to examine the role of JAK1/STAT1 pathway in vivo. Results: Inhibitors of JAK1 and STAT1 significantly reduced the proliferation and activation of CD8+ T cell. Co-culture with CD8+ T cells increased apoptosis and inhibited the proliferation, migration, and invasion of Renca cells. The effects were diminished by JAK1 and STAT1 inhibitors, confirming that CD8+ T cells exert antitumor effects through the JAK1/STAT1 pathway. In vivo, inhibition of this pathway reduced the anti-tumor effects of CD8+ T cells. Conclusion: Inhibitors of JAK1 and STAT1 weakened the antitumor effects of CD8+ T cells, suggesting that targeting this pathway could enhance CD8+ T cell-mediated immunity in renal cancer. | ||
| کلیدواژه‌ها | ||
| CD8+ T Cell؛ Immunity؛ JAK/STAT Pathway؛ Renal Cancer | ||
|
آمار تعداد مشاهده مقاله: 4,868 تعداد دریافت فایل اصل مقاله: 335 |
||