پیوندهای مفید
آمار
| تعداد نشریات | 20 |
| تعداد شماره‌ها | 1,173 |
| تعداد مقالات | 10,715 |
| تعداد مشاهده مقاله | 63,459,587 |
| تعداد دریافت فایل اصل مقاله | 12,362,668 |
miR-196b-5p Affects Macrophage Polarization and Inflammation in Endometriosis | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 3، آذر 2024، صفحه 201-211 اصل مقاله (9.45 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.102744.2794 | ||
| نویسندگان | ||
| Zhe Xue1؛ Yuyan Guo2؛ Fangyun Wang1؛ Qinping Yang1؛ Qiuhong Chen1؛ Tingting Lin1؛ Shunhe Lin* 3 | ||
| 1Department of Obstetrics and Gynecology, Fuqing Maternal and Child Health Hospital, No 1 Furen Road, Fuqing 350399, China. | ||
| 2Physical Examination Center, Fujian Medical University Union Hospital, No 29 Xinquan Road, Fuzhou 350001, China. | ||
| 3Department of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350001, China. | ||
| چکیده | ||
| Background: miR-196b-5p was found to be significantly reduced in endometriosis, but its function and the mechanisms involved remained unclear. Objective: To explore the effect of miR-196b-5p on manipulating macrophage phenotype and the underlying mechanisms in endometriosis Methods: The endometriosis mice and End1/E6E7 cells were used for in vivo and in vitro experiments, respectively. QRT-PCR was used to detect miR-196b-5p, suppressor of cytokine signaling 1 (SOCS1), high mobility group AT-Hook 1 (HMGA1), and CCL2 expressions. Western blot was used to detect SOCS1 and HMGA1 protein levels while luciferase reporter assay was performed to determine the interaction between miR-196b-5p and SOCS1/ HMGA1. ELISA was used to measure CCL2, IL-10, and IL-6 levels and immunohistochemical staining and flow cytometry were used to examine CD86 and CD206 expressions. Results: Significantly reduced levels of miR-196b-5p, and increased levels of SOCS1, HMGA1, and CCL2 were observed in the ectopic endometrium of mice with endometriosis. The miR-196b-5p mimic significantly reduced the lesion size, increased M1 macrophages, and decreased M2 macrophages in the ectopic endometrium of mice with endometriosis. End1/E6E7 cells transfected with miR196b-5p mimic significantly increased M1 macrophages, decreased M2 macrophages and reduced the migration in PMA-treated THP1 cells. Conversely, transfection with a miR-196b-5p inhibitor led to the opposite outcomes. miR-196b-5p targeted SOCS1/HMGA1, and miR-196b-5p inhibitor significantly up-regulated CCL2 and IL-10, and down-regulated IL-6 levels in End1/E6E7 cells. These effects were markedly reversed by si-SOCS1/si-HMGA1. Conclusion: miR-196b-5p elevates M1 macrophages and decreases M2 macrophages in endometriosis, possibly by targeting SOCS1/ HMGA1. This research may provide a novel insight into the pathological mechanisms of endometriosis. | ||
| کلیدواژه‌ها | ||
| Endometriosis؛ Inflammation؛ Macrophages؛ miR-196b-5p | ||
|
آمار تعداد مشاهده مقاله: 702 تعداد دریافت فایل اصل مقاله: 419 |
||