تعداد نشریات | 20 |
تعداد شمارهها | 1,149 |
تعداد مقالات | 10,518 |
تعداد مشاهده مقاله | 45,417,492 |
تعداد دریافت فایل اصل مقاله | 11,295,511 |
The interplay of neutrophils, platelets, and cytokines in the healing process of acetic acid-induced stomach ulcer on trivalent chromium-exposed mice | ||
Iranian Journal of Colorectal Research | ||
مقاله 2، دوره 12، شماره 2، شهریور 2024، صفحه 40-50 اصل مقاله (2.71 M) | ||
نوع مقاله: Research/Original Article | ||
شناسه دیجیتال (DOI): 10.30476/acrr.2024.102723.1219 | ||
نویسندگان | ||
Olugbenga Odukanmi* ؛ Samuel Babafemi Olaleye | ||
Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria | ||
چکیده | ||
Abstract Background: The trace element trivalent chromium has been reported for its beneficial role in experimental colitis and normal gastrointestinal tissues. This study aimed to investigate the effect of trivalent chromium on the injured stomach in mice. Methods: Sixty male slc:ddY mice were used. The animals were randomized into three groups of 20 mice (the control, 10ppm, and 100ppm). Five mice each of twenty were sacrificed on days 0 (for the cytokine study), 3, 7, and 14 and examined. Following anesthesia, the ulcer was induced with acetic acid via laparotomy and through the intraluminal route post-chromium exposure. The blood was obtained from cardiac puncture, and the stomach tissue was scored and excised for myeloperoxidase, catalase, superoxide dismutase, malondialdehyde, total nitrite, cytokines assays, and histology. Results: Ulcer scores of the chromium-exposed reduced significantly compared to the control. The platelets increased dramatically in 10- and 100 ppm compared to control on day 1. There were decreasing neutrophils in the test groups compared to the control across the days of investigation. The malondialdehyde values (nmol/mg protein) significantly reduced in the 10ppm (6.42 ± 0.24+; 3.70±0.42+) and 100ppm (5.22 ± 0.47+; 2.95 ± 0.31+) groups compared to the control (8.35 ± 0.43; 4.53 ± 0.48) on days 7 and 14 respectively. Superoxide dismutase increased, (p = 0.0372, 0.0441, and 0.0421 on days 3, 7, and 14, respectively) in the chromium groups compared to the control. Myeloperoxidase decreased significantly, p = 0.0466, and 0.0383 on days 3 and 7 in the chromium groups compared to the control group, respectively. There was no significant change with Catalase and Nitrates assessed. IL-1α, TNF-α, and interferon-γ reduced while IL-10 increased in the mRNA chromium exposed. Conclusion: Chromium exposure to mice upgrades the restoration of gastric ulcers in mice by inhibiting reactive oxygen radicals while promoting endogenous antioxidants | ||
کلیدواژهها | ||
trivalent chromium؛ reactive oxygen species؛ inflammation؛ cytokines؛ healing؛ ulcer | ||
مراجع | ||
1. Lassen A, Hallas J, De Muckadell O.B.S. Complicated a nd uncomplicated peptic ulcers in a Danish county 1993-2002: a population-based cohort study. Am J Gastroenterol 2006; 101: 945–953. 2. Xia B, Xia H.H.X, Ma C.W, et al. Trends in the prevalence of peptic ulcer disease and Helicobacter pylori infection in family physician-referred uninvestigated dyspeptic patients in Hong Kong. Aliment Pharmacol Ther 2005; 22: 243–249. 3. Schiffmann L, Kahrau S, Berger G, et al. Colon perforation in an adolescent after short-term diclofenac intake. ANZ J Surg 2005; 75: 726–727. 4. McCarthy D.M. Prevention and treatment of gastrointestinal symptoms and complications due to NSAIDs. Best Pract Res Clin Gastroenterol 2001; 15: 755–773. 5. Lambert M, Leven B, technology RG-E science and, et al. New methods of cleaning up heavy metal in soils and water. Citeseer, http://citeseerx. ist.psu.edu/viewdoc/download?doi= 10.1.1.400.2031&rep=rep1&type=pdf (accessed 14 October 2021). 6. Jaishankar M, Tseten T, Anbalagan N, et al. Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol 2014; 7: 60. 7. Losacco C, Perillo A. Metal-induced oxidative Stress and Cellular Signaling Alteration in Animals. Iran J Appl Anim Sci 2018; 8: 367–373. 8. Odukanmi O. A, Salami A.T, Koda K, et al. Trivalent Chromium Promotes Healing of Experimental Colitis in Mice by Suppression of Inflammation and Oxidative Stress. J Biosci Med 2017; 5: 108–126. 9. Mertz W. Interaction of chromium with insulin: A progress report. Nutr Rev 1998; 56: 174–177. 10. Lv H, Lin Y, Liu P, et al. Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice. Acta Biochim Biophys Sin (Shanghai) 2019; 51: 925–933. 11. Yazdanpanah K, Parhizkar B, Sheikhesmaeili F, et al. Efficacy of zinc sulfate in peptic ulcer disease: A randomized double-blind clinical trial study. J Clin Diagnostic Res 2016; 10: OC11–OC15. 12. Chromium - Health Professional Fact Sheet, https://ods.od.nih. g o v / f a c t s h e e t s / C h r o m i u m - HealthProfessional/ (accessed 17 October 2021). 13. Sazakli E, Villanueva C.M., Kogevinas M, Maltezis K, Mouzaki A. Chromium in Drinking Water: Association with Biomarkers of Exposure and Effect. Int J Environ Res Publ Heal 2014; 11: 10125–10145. 14. Vincent J.B, Lukaski H. C. Chromium. Adv Nutr 2018; 9: 505–506. 15. Scientific Opinion on Dietary Reference Values for chromium. EFSA J; 12. Epub ahead of print 1 October 2014. DOI: 10.2903/J. EFSA.2014.3845. 16. Federal Register : Food Labeling: Revision of the Nutrition and Supplement Facts Labels, https:// w w w . f e d e r a l r e g i s t e r . g o v / documents/2016/05/27/2016-11867/ food-labeli ng-revision- of-the- nutrition-and-supplement-facts-labels (accessed 17 October 2021). 17. Vincent J.B. The potential value and toxicity of chromium picolinate as a nutritional supplement, weight loss agent and muscle development agent. Sports Med 2003; 33: 213–230. 18. Nakada M, Demura S. The effect of past and present lifestyle, nutrition habits, and gender on bone mineral density. Health (Irvine Calif) 2010; 02: 713–721. 19. Odukanmi O.A, Owoeye O, Salami A. T, Morakinyo O.L., Iyiola O.T and Olaleye S.B. Effect of trivalent chromium (Cr2O3 ) on stomach morphometry and some vital organs in male wistar rats | African Journal of Medicine and Medical Sciences. Afr J Med Med Sci 2019; 48: 329–337. 20. Kareema H, Dohan A-H, Abbas H. Effects of Potassium Dichromate on Reproduction and Fertility in Albino Female Mice. Int J PharmTech Res 2016; 2016: 205–213. 21. Amagase K, Okabe S. Acetic acid ulcers: a new method for producing solitary chronic ulcers in rat stomachs by intraluminal application of acetic acid solution. InflammoPharmacology 2002 104 2002; 10: 385–389. 22. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins using the biuret reaction. Epub ahead of print 1949. DOI: 10.1016/ S0021-9258(18)57021-6. 23. Varshney R, Kale R.K. Effects of calmodulin antagonists on radiation- induced lipid peroxidation in microsomes. Int J Radiat Biol 1990; 58: 733–743. 24. Sinha A.K. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389–394. 25. Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44: 276–287. 26. Ignarro, L.J., Buga, G.M., Wood, K.S., Byrns, R.E., and Chaudhuri, G. 1987. Endothelium-Derived relaxing factor produced and released from artery and vein is nitric oxide. Proc. Natl. Acad. Sci. U.S.A. 84(24): 9265–9269 27. Kim H, Wei Y, Lee JY, et al. Myeloperoxidase Inhibition Increases Neurogenesis after Ischemic Stroke. J Pharmacol Exp Ther 2016; 359: 262. 28. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002; 3: 1–12. 29. Animals NRC (US.) C for the U of the G for the C and U of L. Guide for the Care and Use of Laboratory Animals. Guid Care Use Lab Anim. Epub ahead of print 27 December 2011. DOI: 10.17226/12910. 30. Qing C. The molecular biology in wound healing & non-healing wound. Chinese J Traumatol 2017; 20: 189–193. 31. Phillipson M, Kubes P. The neutrophil in vascular inflammation. Nat Med 2011; 17: 1381–1390. 32. Peiseler M, Kubes P. More friend than foe: The emerging role of neutrophils in tissue repair. J Clin Invest 2019; 129: 2629–2639. 33. Hottz ED, Paula Monteiro AT, Bozza FA, et al. Review Article Inflammasome in Platelets: Allying Coagulation and Inflammation in Infectious and Sterile Diseases? Epub ahead of print 2015. DOI: 10.1155/2015/435783. 34. Andia I, Sanchez M, Maffulli N. Tendon healing and platelet-rich plasma therapies. Expert Opin Biol Ther 2010; 10: 1415–1426. 35. Halpern BC, Chaudhury S, Rodeo SA. The Role of Platelet-Rich Plasma in Inducing Musculoskeletal Tissue Healing: https://doi.org/101007/ s11420-011-9239-7 2012; 8: 137–145. 36. Davis VL, Abukabda AB, Radio NM, et al. Platelet-rich preparations to improve healing. Part II: platelet activation and enrichment, leukocyte inclusion, and other selection criteria. J Oral Implantol 2014; 40: 511–521. 37. Demidova-Rice TN, Hamblin MR, Herman IM. Acute and impaired wound healing: pathophysiology and current methods for drug delivery, part 1: normal and chronic wounds: biology, causes, and approaches to care. Adv Skin Wound Care 2012; 25: 304–314. 38. Zimmerman G.A, Weyrich A.S. Signal-Dependent Protein Synthesis by Activated Platelets New Pathways to Altered Phenotype and Function. Arterioscler Thromb Vasc Biol 2008; 28: s17. 39. Parrish W.R. Physiology of Blood Components in Wound Healing: an Appreciation of Cellular Co-Operativity in Platelet Rich Plasma Action. J Exerc Sport Orthop 2017; 4: 1–14. 40. Conti V, Izzo V, Corbi G, et al. Antioxidant Supplementation in the Treatment of Aging-Associated Diseases. Front Pharmacol; 7. Epub ahead of print 12 February 2016. DOI: 10.3389/FPHAR.2016.00024. 41. Hwang J, Jin J, Jeon S, et al. SOD1 suppresses pro-inflammatory immune responses by protecting against oxidative stress in colitis. Redox Biol 2020; 37: 101760. 42. Balali-Mood M, Naseri K, Tahergorabi Z, et al. Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Front Pharmacol 2021; 12: 227. 43. Kangralkar, V.A, Patil, S.D, Bandivadekar R. Oxidative stress and diabetes: a review. Int J Pharm Appl 2010; 1: 38-45. 44. Porfire A.S, Leucuţa SE, Kiss B, et al. Investigation into the role of Cu/Zn-SOD delivery system on its antioxidant and anti-inflammatory activity in rat model of peritonitis. Pharmacol Rep 2014; 66: 670–676. 45. Satish L, Kathju S. Cellular and Molecular Characteristics of Scarless versus Fibrotic Wound Healing. Dermatol Res Pract; 2010. Epub ahead of print 2010. DOI: 10.1155/2010/790234. 46. Schultz G.S, Chin G.A, Moldawer L, et al. Principles of Wound Healing. Diabet Foot Probl 2011; 395–402. 47. Sziksz E, Pap D, Lippai R, et al. Fibrosis Related Inf lammatory Mediators: Role of the IL-10 Cytokine Family. Mediators Inflamm; 2015. Epub ahead of print 2015. DOI: 10.1155/2015/764641 | ||
آمار تعداد مشاهده مقاله: 58 تعداد دریافت فایل اصل مقاله: 94 |