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Sankar, Sudharsan, Redipalli, Sharmishta, Jayaraj, Indhuja, Ghosal, Soumika, Parvathi, Venkatachalam Deepa. (1403). Evaluating the Genotoxicity of Hydroxychloroquine: An In Vitro and In Vivo Study. سامانه مدیریت نشریات علمی, 12(3), 251-259. doi: 10.30476/jhsss.2023.99791.1815
Sudharsan Sankar; Sharmishta Redipalli; Indhuja Jayaraj; Soumika Ghosal; Venkatachalam Deepa Parvathi. "Evaluating the Genotoxicity of Hydroxychloroquine: An In Vitro and In Vivo Study". سامانه مدیریت نشریات علمی, 12, 3, 1403, 251-259. doi: 10.30476/jhsss.2023.99791.1815
Sankar, Sudharsan, Redipalli, Sharmishta, Jayaraj, Indhuja, Ghosal, Soumika, Parvathi, Venkatachalam Deepa. (1403). 'Evaluating the Genotoxicity of Hydroxychloroquine: An In Vitro and In Vivo Study', سامانه مدیریت نشریات علمی, 12(3), pp. 251-259. doi: 10.30476/jhsss.2023.99791.1815
Sankar, Sudharsan, Redipalli, Sharmishta, Jayaraj, Indhuja, Ghosal, Soumika, Parvathi, Venkatachalam Deepa. Evaluating the Genotoxicity of Hydroxychloroquine: An In Vitro and In Vivo Study. سامانه مدیریت نشریات علمی, 1403; 12(3): 251-259. doi: 10.30476/jhsss.2023.99791.1815

Evaluating the Genotoxicity of Hydroxychloroquine: An In Vitro and In Vivo Study

Journal of Health Sciences & Surveillance System
دوره 12، شماره 3، مهر 2024، صفحه 251-259    اصل مقاله (1.34 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/jhsss.2023.99791.1815
نویسندگان
Sudharsan Sankar1؛ Sharmishta Redipalli1؛ Indhuja Jayaraj2؛ Soumika Ghosal1؛ Venkatachalam Deepa Parvathi* 1
1Department of Biomedical Sciences, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Ramachandra Nagar, Porur, Chennai-600116 Tamil Nadu, India
21Department of Biomedical Sciences, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Ramachandra Nagar, Porur, Chennai-600116 Tamil Nadu, India
چکیده
Background: Hydroxychloroquine (HCQ) is a drug used to treat malarial parasites and it was extensively used during the initial phase of COVID-19. However, HCQ demonstrated certain serious effects when administered to patients. Hence, this study intends to determine its toxicity by exposing it to human peripheral blood and the fly model.
Methods: In the present experimental study, HCQ (200 mg) was tested in different volumes (62.5 μl - 500 μl) on human blood samples (in vitro) and Drosophila melanogaster (in vivo). Hemolytic assay, trypan blue assay, mitotic index, chromosomal aberration, and DNA fragmentation assay were used to assess the sublethal effects of HCQ.
Results: The results implied that HCQ, at its highest concentration (500 μl), showed maximum lysis in the hemolytic assay, and an increased number of dead cells were observed with increasing concentration in trypan blue assay. Also, the percent mitotic index decreased with increasing concentration of HCQ. Chromosomal aberrations, including breaks, centromeric disruption, dicentrics, and pulverized chromosomes, were observed on exposure to HCQ. The number of fragments in agarose gel electrophoresis revealed damage to DNA. Therefore, these results provide evidence and prove the cytotoxicity and genotoxicity of HCQ.
Conclusion: HCQ is found to have cytotoxic and genotoxic effects. These results imply that further examinations must be conducted before prescribing HCQ to treat various diseases.

تازه های تحقیق

Sudharsan Sankar (Google Scholar)

Venkatachalam Deepa Parvathi (Google Scholar)

کلیدواژه‌ها
Drosophila melanogaster؛ Cytotoxicity؛ Human peripheral blood؛ Genotoxicity؛ Hydroxychloroquine
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