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The Effect of Interferon Beta and Natalizumab on miR-20b Expression in Patients with Relapsing-Remitting Multiple Sclerosis is Potentially Mediated by Modulation of the Jak–STAT Signaling Pathway: A Case-control Study | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 2، شهریور 2024، صفحه 158-165 اصل مقاله (559.03 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.100500.2694 | ||
| نویسندگان | ||
| Aysan Jafari Harandi1، 2؛ Alireza Mirzaee Sedigh1؛ Mitra Ataei1؛ Sepideh Bayrami2؛ Emran Esmaeilzadeh2؛ Mohammad Hossein Sanati* 1 | ||
| 1Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran. | ||
| 2Fetal Health Research Center, Hope Generation Foundation, Tehran, Iran. | ||
| چکیده | ||
| Background: The mechanisms of the function of interferon beta (IFN-β) and natalizumab (NTZ) in multiple sclerosis (MS) patients have not yet been fully understood. Over the past decades, many studies have been conducted to evaluate gene expression changes especially regulatory non-coding RNAs such as microRNAs (miRNAs) following therapy in MS patients. Objective: To assess the changes in the expression of miR-20b in MS patients treated with IFN-β or NTZ. Methods: Sixty patients with relapsing-remitting MS (RRMS) and 30 healthy controls (HCs) were enrolled. The patients were categorized as untreated (N=20), IFN-β-treated (N=20), and NTZtreated (N=20). For the expression analysis, real-time PCR was performed on the whole blood. The bioinformatic tools were applied for signaling pathways enrichment analysis of miR-20b targetome. Results: The relative expression of miR-20b was significantly downregulated in the untreated patients compared with the HCs (-1.726-fold, p<0.001), while IFN-β-treated and NTZ-treated patients showed no statistical difference compared with the HCs (0.733-fold, p=0.99 for IFN-β and 1.025-fold, p=0.18 for NTZ). This indicates the restoration of miR-20b expression to normal level in the treated patients. Additionally, in silico analysis demonstrated that the Jak–STAT signaling pathway is enriched with miR-20b targets (p<0.0001). Conclusion: Our findings suggest that the positive effects of IFN-β and NTZ in the RRMS patients could be potentially mediated by returning miR-20b expression to baseline. | ||
| کلیدواژه‌ها | ||
| Interferon Beta؛ Natalizumab؛ miR-20b؛ Multiple Sclerosis | ||
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آمار تعداد مشاهده مقاله: 400 تعداد دریافت فایل اصل مقاله: 364 |
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