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Alsaffar, Sura F., Baqer, Noor Nihad. (1403). Association between Methylenetetrahydrofolate Reductase (MTHFR( and 5-Methyltetrahydrofolate- Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19. سامانه مدیریت نشریات علمی, 49(7), 430-440. doi: 10.30476/ijms.2023.99555.3162
Sura F. Alsaffar; Noor Nihad Baqer. "Association between Methylenetetrahydrofolate Reductase (MTHFR( and 5-Methyltetrahydrofolate- Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19". سامانه مدیریت نشریات علمی, 49, 7, 1403, 430-440. doi: 10.30476/ijms.2023.99555.3162
Alsaffar, Sura F., Baqer, Noor Nihad. (1403). 'Association between Methylenetetrahydrofolate Reductase (MTHFR( and 5-Methyltetrahydrofolate- Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19', سامانه مدیریت نشریات علمی, 49(7), pp. 430-440. doi: 10.30476/ijms.2023.99555.3162
Alsaffar, Sura F., Baqer, Noor Nihad. Association between Methylenetetrahydrofolate Reductase (MTHFR( and 5-Methyltetrahydrofolate- Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19. سامانه مدیریت نشریات علمی, 1403; 49(7): 430-440. doi: 10.30476/ijms.2023.99555.3162

Association between Methylenetetrahydrofolate Reductase (MTHFR( and 5-Methyltetrahydrofolate- Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19

Iranian Journal of Medical Sciences
مقاله 4، دوره 49، شماره 7، مهر 2024، صفحه 430-440    اصل مقاله (873.8 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.99555.3162
نویسندگان
Sura F. Alsaffar1؛ Noor Nihad Baqer* 2
1Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
2Ministry of Science and Technology, Baghdad, Iraq
چکیده
Background: The methylenetetrahydrofolate reductase (MTHFR) gene is an essential gene in the metabolism of folate-homocysteine. Recently, the level of homocysteine was found to be a significant marker in the follow-up of COVID-19 infection. Thus, this study aimed to detect the effect of genetic polymorphisms for single nucleotide polymorphisms (SNPs) (c.66A>G, c.1298A>C, and c.677CT) on COVID-19 infection. 
Methods: Blood samples were collected from 270 patients with COVID-19 in the medical center of Al-Shifa (Baghdad, Iraq) from November 2020 to March 2021. Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for the detection of genotypes of SNPs. The odds ratio (OR) was used to detect the relationship between SNPs and COVID-19 infections. Haplotype analysis was performed by SHEsis software.
Results: There was a significant difference between mild/moderate cases and severe/critical cases for ages (35-45), (46-55), and (56-65) years (P<0.0001, P=0.01, and P=0.006, respectively). The results showed significant differences in the T allele for SNP c.677>C (P<0.0001 and OR=4.58). The C allele for SNP c.1298A>C indicated significant differences (P<0.001 and OR=3.15). Besides, the G allele for SNP c.677C>T showed significant differences (P<0.001 and OR=6.64). Consequently, these SNPs showed a predisposition to the development of COVID-19 infection. With regard to the C-A-A, T-A-A and T-C-G haplotypes indicated significant differences between the control and patient groups. The C-A-A was related to a decreased risk and indicated a protective effect against COVID-19 infection development (P<0.0001 and OR=0.218). The increased risk was associated with T-A-A and T-C-G haplotypes and indicated the risk impact on COVID-19 infection development (P<0.0001, P=0.004, and OR=15.5, OR=6.772, respectively). Furthermore, the linkage disequilibrium (LD) for SNPs was studied, and the complete D’ value was 99%.
Conclusion: The genetic polymorphisms of SNPs (c.66A>G, c.1298A>C, and c.677C>T) in the Iraqi population were associated with COVID-19 infection.
کلیدواژه‌ها
COVID-19؛ Polymorphism, single nucleotide؛ MTHFR gene؛ MTRR gene
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