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Johdi, Nor Adzimah, Seng, Amanda, Lee, Wei-Kang, Mohamad Said, Hanif Zulkhairi, Wan Jamaluddin, Wan Fariza. (1403). Exploring Differentially Expressed Genes and Immune Modulation in Diffuse Large B-Cell Lymphoma through RNA Sequencing Analysis. سامانه مدیریت نشریات علمی, 49(10), 652-660. doi: 10.30476/ijms.2023.100149.3234
Nor Adzimah Johdi; Amanda Seng; Wei-Kang Lee; Hanif Zulkhairi Mohamad Said; Wan Fariza Wan Jamaluddin. "Exploring Differentially Expressed Genes and Immune Modulation in Diffuse Large B-Cell Lymphoma through RNA Sequencing Analysis". سامانه مدیریت نشریات علمی, 49, 10, 1403, 652-660. doi: 10.30476/ijms.2023.100149.3234
Johdi, Nor Adzimah, Seng, Amanda, Lee, Wei-Kang, Mohamad Said, Hanif Zulkhairi, Wan Jamaluddin, Wan Fariza. (1403). 'Exploring Differentially Expressed Genes and Immune Modulation in Diffuse Large B-Cell Lymphoma through RNA Sequencing Analysis', سامانه مدیریت نشریات علمی, 49(10), pp. 652-660. doi: 10.30476/ijms.2023.100149.3234
Johdi, Nor Adzimah, Seng, Amanda, Lee, Wei-Kang, Mohamad Said, Hanif Zulkhairi, Wan Jamaluddin, Wan Fariza. Exploring Differentially Expressed Genes and Immune Modulation in Diffuse Large B-Cell Lymphoma through RNA Sequencing Analysis. سامانه مدیریت نشریات علمی, 1403; 49(10): 652-660. doi: 10.30476/ijms.2023.100149.3234

Exploring Differentially Expressed Genes and Immune Modulation in Diffuse Large B-Cell Lymphoma through RNA Sequencing Analysis

Iranian Journal of Medical Sciences
مقاله 5، دوره 49، شماره 10، دی 2024، صفحه 652-660    اصل مقاله (2.81 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.100149.3234
نویسندگان
Nor Adzimah Johdi* 1؛ Amanda Seng2؛ Wei-Kang Lee2؛ Hanif Zulkhairi Mohamad Said1؛ Wan Fariza Wan Jamaluddin3
1UKM Medical Molecular Biology Institute, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia
2Codon Genomics Sdn Bhd, Seri Kembangan Selangor Darul Ehsan, Malaysia
3Cell Therapy Center, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia
چکیده
Background: Diffuse large B-cell lymphoma (DLBCL) is globally recognized as the most prevalent and aggressive subtype of non-Hodgkin lymphoma. While conventional treatments are effective initially, the disease can become resistant or relapse over time. The study aimed to examine the differentially expressed genes at the transcriptome level and molecular pathways in DLBCL patients. 
Methods: This investigation utilized RNA sequencing analysis to compare differentially expressed gene samples from five diffuse large B-cell lymphoma patients with two healthy volunteers. These participants were admitted to UKM Medical Center, Kuala Lumpur between 2019 and 2020. The differentially expressed genes were conducted using the DESeq2 R package (version 1.10.1) using a negative binomial distribution model. The obtained P values were corrected with the Benjamin and Hochberg method and identified using a False Discovery Rate threshold of <0.05, with log2 fold change (FC) of ≥2 or ≤-2. 
Results: Results showed 73 differentially expressed genes between the two groups, among which 70 genes were downregulated, and three genes were upregulated. The differentially expressed genes analyzed with the Reactome pathway were significantly associated with the downregulation of antimicrobial humoral response (P<0.001), neutrophil degranulation (P<0.001), chemokine receptors bind chemokines (P=0.028), defensins (P=0.028) and metabolism of angiotensinogen (P=0.040). 
Conclusion: These findings suggest that the identified pathways may contribute to cancer progression and weaken the immune response in diffuse large B-cell lymphoma patients. This study offers fresh insights into previously undiscovered downstream targets and pathways modulated by diffuse large B-cell lymphoma.
کلیدواژه‌ها
Diffuse large B-cell lymphoma؛ RNA sequencing؛ Gene ontology؛ Transcriptome؛ Immunity
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مراجع
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