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Shaker, Nada Sahib, Sahib, Hayder B, Tahseen, Nibras J. (1403). Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19. سامانه مدیریت نشریات علمی, 49(5), 322-331. doi: 10.30476/ijms.2023.98947.3102
Nada Sahib Shaker; Hayder B Sahib; Nibras J Tahseen. "Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19". سامانه مدیریت نشریات علمی, 49, 5, 1403, 322-331. doi: 10.30476/ijms.2023.98947.3102
Shaker, Nada Sahib, Sahib, Hayder B, Tahseen, Nibras J. (1403). 'Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19', سامانه مدیریت نشریات علمی, 49(5), pp. 322-331. doi: 10.30476/ijms.2023.98947.3102
Shaker, Nada Sahib, Sahib, Hayder B, Tahseen, Nibras J. Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19. سامانه مدیریت نشریات علمی, 1403; 49(5): 322-331. doi: 10.30476/ijms.2023.98947.3102

Anti-cytokine Storm Activity of Fraxin, Quercetin, and their Combination on Lipopolysaccharide-Induced Cytokine Storm in Mice: Implications in COVID-19

Iranian Journal of Medical Sciences
مقاله 6، دوره 49، شماره 5، مرداد 2024، صفحه 322-331    اصل مقاله (3.26 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.98947.3102
نویسندگان
Nada Sahib Shaker* 1؛ Hayder B Sahib2؛ Nibras J Tahseen3
1Department of Pharmacology and Toxicology, Mustansiriyah University, College of Pharmacy, Baghdad, Iraq
2Dean of College of Pharmacy, Al-Nahrain University, Baghdad, Iraq
3Al-Bayan University, College of Pharmacy, Baghdad, Iraq
چکیده
Background: Cytokine release syndrome (CRS) is the leading cause of mortality in advanced stages of coronavirus patients. This study examined the prophylactic effects of fraxin, quercetin, and a combination of fraxin+quercetin (FQ) on lipopolysaccharide-induced mice.
Methods: Sixty mice were divided into six groups (n=10) as follows: control, LPS only, fraxin (120 mg/Kg), quercetin (100 mg/Kg), dexamethasone (5 mg/Kg), and FQ. All treatments were administered intraperitoneally (IP) one hour before induction by LPS (5 mg/Kg) IP injection. Twenty-four hours later, the mice were euthanized. Interleukin one beta (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were quantified using an enzyme-linked immunosorbent assay (ELISA), and lung and kidney tissues were examined for histopathological alterations. This study was conducted at Al-Nahrain University, Baghdad, Iraq, in 2022. 
Results: FQ reduced IL-1β (P<0.001). All treatments significantly suppressed IL-6, fraxin, quercetin, dexamethasone, and FQ, all with P<0.001. The TNF-α level was reduced more with dexamethasone (P<0.001) and quercetin (P<0.001). Histopathological scores were significantly reduced mainly by quercetin and FQ in the lungs with scores of 12.30±0.20 (P=0.093), and 15.70±0.20 (P=0.531), respectively. The scores were 13±0.26 (P=0.074) and 15±0.26 (P=0.222) for quercetin and FQ in the kidneys, respectively.
Conclusion: All used treatments reduced proinflammatory cytokine levels and protected against LPS-induced tissue damage.
کلیدواژه‌ها
Quercetin؛ Cytokine release syndrome؛ Lipopolysaccharides؛ Fraxin؛ COVID-19
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