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Annita, Annita, Revilla, Gusti, Ali, Hirowati, Almurdi, Almurdi. (1403). The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer’s-like Pathology in a Rat Model. سامانه مدیریت نشریات علمی, 49(7), 441-449. doi: 10.30476/ijms.2023.98912.3104
Annita Annita; Gusti Revilla; Hirowati Ali; Almurdi Almurdi. "The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer’s-like Pathology in a Rat Model". سامانه مدیریت نشریات علمی, 49, 7, 1403, 441-449. doi: 10.30476/ijms.2023.98912.3104
Annita, Annita, Revilla, Gusti, Ali, Hirowati, Almurdi, Almurdi. (1403). 'The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer’s-like Pathology in a Rat Model', سامانه مدیریت نشریات علمی, 49(7), pp. 441-449. doi: 10.30476/ijms.2023.98912.3104
Annita, Annita, Revilla, Gusti, Ali, Hirowati, Almurdi, Almurdi. The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer’s-like Pathology in a Rat Model. سامانه مدیریت نشریات علمی, 1403; 49(7): 441-449. doi: 10.30476/ijms.2023.98912.3104

The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer’s-like Pathology in a Rat Model

Iranian Journal of Medical Sciences
مقاله 5، دوره 49، شماره 7، مهر 2024، صفحه 441-449    اصل مقاله (728.37 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.98912.3104
نویسندگان
Annita Annita* 1، 2؛ Gusti Revilla3؛ Hirowati Ali4؛ Almurdi Almurdi5
1Doctoral Program Biomedical Science, Faculty of Medicine, Andalas University, Padang, Indonesia
2Department of Medical Laboratory Technology, STIKES Syedza Saintika, Indonesia
3Department of Anatomy, Faculty of Medicine, Andalas University, Padang, Indonesia
4Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
5Clinical Pathology, Faculty of Medicine, Andalas University, Padang, Indonesia
چکیده
Background: Alzheimer’s disease (AD) is a neurodegenerative condition characterized by gradual cognitive impairment, including loss of synapses and nerve cells involved in learning, memory, and habit formation processes. Bone Marrow Mesenchymal Stem Cells (BM-MSCs) are multipotent cells. Because of their self-renewable, differentiation, and immunomodulatory capabilities, they are commonly used to treat many disorders. Hence, the current study intends to examine the effect of BM-MSCs transplantation on Aluminum chloride (AlCl3)-induced cognitive problems, an experimental model resembling AD’s hallmarks in rats.
Methods: The study was conducted in 2022 at The Biomedical Laboratory Faculty of Medicine, Andalas University, Indonesia. Adult male Wistar rats (three groups: negative control; no intervention+treatment with PBS; positive control: AlCl3+treatment with aqua dest; AlCl3+BM-MSCs: AlCl3+treatment with BM-MSCs, n=5 each) were treated daily with AlCl3 orally for five days. Stem cells were intraperitoneally injected into rats at a dose of 1x106 cells/rat. The same quantity of phosphate-buffered saline was given to the control group. One month after stem cell injection, the rat brain tissue was removed and placed in the film bottles that had been created. The expression of neural progenitor cell markers, including nestin and sex-determining Y-box 2 (SOX-2), was analyzed using real-time polymerase chain reaction (RT-PCR). Rats’ cognitive and functional memory were examined using Y-maze. Data were analyzed using SPSS software (version 26.0) with a one-way analysis of variance (ANOVA) test.
Results: The gene expression of nestin (29.74±0.42), SOX-2 (31.44±0.67), and percent alternation of Y-maze (67.04±2.28) increased in the AlCl3+BM-MSCs group compared to that in the positive control group. RT-PCR analysis indicated that nestin (P<0.001) and SOX-2 (P<0.001) were significantly enhanced in the AlCl3+BM-MSCs group compared to the positive control group. This group also indicated an increased percent alternation of Y-maze (P<0.001) in the AlCl3+BM-MSCs group compared to the positive control group. 
Conclusion: Due to its potential effects on cell therapy, BM-MSCs were found effective in a rat model of AD on the impairment of the rats’ behavior and increased expression of neural progenitor cell markers.
کلیدواژه‌ها
Mesenchymal stem cells؛ Neurodegenerative diseases؛ Aluminum chloride؛ Reverse transcriptase polymerase chain reaction؛ Nestin
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