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MicroRNA-150 Deletion Reduces the Occurrence and Severity of Rheumatoid Arthritis by Inhibiting IL-17 | ||
| Iranian Journal of Immunology | ||
| دوره 21، شماره 1، خرداد 2024، صفحه 89-100 اصل مقاله (5.61 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2024.99855.2666 | ||
| نویسندگان | ||
| Aihong Zhang1؛ Quanhui Zheng* 2 | ||
| 1Department of ICU, The Affiliated Hospital of North China University of Science and Technology, Tangshan, China. | ||
| 2Hebei Key Laboratory for Chronic Diseases, Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Diseases, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, China. | ||
| چکیده | ||
| Background: Understanding the effects of epigenetic factors on the pathogenesis of rheumatoid arthritis (RA) is important for the early diagnosis and therapeutic intervention of this disease. MicroRNA-150 (miR-150) exerts an important influence on the development and function of lymphocytes. However, the role of miR-150 in the pathogenesis of RA remains unclear. Objective: To explore the role of miR-150 in the pathogenesis of RA and the related immune mechanism. Methods: In this study, we used miR-150 knock-out (miR-150KO) and created animal models of RA. Flow cytometry, immunohistochemistry, and real-time RT-PCR were employed to assess the frequency of T cell subsets and cytokines expression. Results: Compared to wild-type (WT) mice, the onset of RA was postponed and the incidence of RA was reduced in miR-150KO mice. The expression of IL-4 and IFN-γ significantly increased while the expression of IL-17 decreased significantly in NKT and CD4+ T cells of KO mice compared to that of WT mice after RA induction. In addition, the expression of IL-4 and IFN-γ increased while the expression of IL-17 decreased significantly in the joint tissues of KO mice compared to that of WT mice. Furthermore, the mRNA expression of TNF-α and IL-17 decreased significantly in the synovial fluid cells of KO mice compared to that of the WT mice after RA induction. Conclusion: MiR-150 deficiency decreases the expression of IL-17 in T cells and joint tissues, and alleviates the occurrence and progression of RA in mice. | ||
| کلیدواژه‌ها | ||
| IL-17؛ MicroRNA-150؛ Rheumatoid Arthritis | ||
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آمار تعداد مشاهده مقاله: 521 تعداد دریافت فایل اصل مقاله: 385 |
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