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In Vitro Assessment of Morphology, Proliferation, Apoptosis and Differential Potential of Dental Pulp Stem Cells, When Marijuana Is Added to Nutrients of Cell Culture Medium | ||
| International Journal of Nutrition Sciences | ||
| مقاله 8، دوره 9، شماره 1، خرداد 2024، صفحه 62-70 اصل مقاله (1.32 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/ijns.2024.101034.1288 | ||
| نویسندگان | ||
| Mina Abroudi1، 2؛ Davood Mehrabani* 2، 3، 4، 5؛ Shahrokh Zare2؛ Iman Jamhiri2؛ Marzieh Hamzavai6، 7؛ Shimasadat Masoumi6؛ Golshid Mehrabani6، 8؛ Atefehsadat Masoumi9؛ Seyedeh Sara Hashemi3؛ Feridoun Karimi-Busheri5 | ||
| 1Department of Biology, Shiraz Islamic Azad University, Shiraz, Iran | ||
| 2Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 4Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 5Department of Oncology, Cross Cancer Institute, Faculty of Medicine, University of Alberta, Edmonton, AB, Canada | ||
| 6School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 7Dr. Hamzavi Dental Clinic Center, Shiraz, Iran | ||
| 8Henry M. Goldman School of Dental Medicine, Boston University, Boston, MA, USA | ||
| 9Faculty of Dentistry, Shiraz Islamic Azad University, Shiraz, Iran | ||
| چکیده | ||
| Background: Cannabis, commonly known as marijuana, is widely used for recreational purposes. It has stimulatory effect on appetite, so cannabinoid receptor antagonists have been used to decrease food intake and to act peripherally by rising thermogenesis and energy expenditure to control obesity. This in vitro study determined morphological, growth, apoptosis and differential potential of changes in dental pulp stem cells (DPSCs) when marijuana was added to nutrients of cell culture medium. Methods: Wisdom teeth extracted were used to obtain DPSCs, while characterized morphologically, by osteo- and adipo-inductions and flowcytometry for mesenchymal properties. MTT assay identified optimal concentration of cannabis extract. Cells were treated with 120 and 1000 ng/mL of cannabis during seven days period, while proliferation, apoptosis and differentiation of DPSCs were assessed. Results: DPSCs were spindle shape and showed mesenchymal characteristics. MTT assay illustrated an increase in cell number until day 5th when DPSCs were treated with 120 and 1000 ng/mL of cannabis, while there was a decreasing trend on day 6th. There was an upregulation of the expression of Bax and COL1A1genes on day 6th when 120 and 1000 ng/mL of cannabis were added to the media in comparison to the control group. Conclusion: The increase in DPSC proliferation and viability when treated with cannabis denotes to its positive impact on cell proliferation during short term period, while a long term exposure to cannabis resulted in apoptosis and a decrease in cell proliferation. These findings reveal an issue of public health concern and alarm for health authorities. | ||
تازه های تحقیق | ||
Mina Abroudi (Google Scholar) Davood Mehrabani (Google Scholar) | ||
| کلیدواژه‌ها | ||
| Cannabis؛ Dental pulp stem cells؛ Proliferation؛ Viability؛ Differentiation | ||
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