LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,183
تعداد مقالات10,793
تعداد مشاهده مقاله72,655,192
تعداد دریافت فایل اصل مقاله12,778,382
Nazemi Salman, Bahareh, Saburi, Ehsan, Mohammadi Gheidari, Mahtab, Farsadeghi, Mahya, Basir Shabestari, Samira. (1403). Cytotoxic Effects of Pulp Capping Agents on Mesenchymal Stem Cells Isolated from Human Exfoliated Deciduous Teeth. سامانه مدیریت نشریات علمی, 25(4), 342-348. doi: 10.30476/dentjods.2023.99173.2131
Bahareh Nazemi Salman; Ehsan Saburi; Mahtab Mohammadi Gheidari; Mahya Farsadeghi; Samira Basir Shabestari. "Cytotoxic Effects of Pulp Capping Agents on Mesenchymal Stem Cells Isolated from Human Exfoliated Deciduous Teeth". سامانه مدیریت نشریات علمی, 25, 4, 1403, 342-348. doi: 10.30476/dentjods.2023.99173.2131
Nazemi Salman, Bahareh, Saburi, Ehsan, Mohammadi Gheidari, Mahtab, Farsadeghi, Mahya, Basir Shabestari, Samira. (1403). 'Cytotoxic Effects of Pulp Capping Agents on Mesenchymal Stem Cells Isolated from Human Exfoliated Deciduous Teeth', سامانه مدیریت نشریات علمی, 25(4), pp. 342-348. doi: 10.30476/dentjods.2023.99173.2131
Nazemi Salman, Bahareh, Saburi, Ehsan, Mohammadi Gheidari, Mahtab, Farsadeghi, Mahya, Basir Shabestari, Samira. Cytotoxic Effects of Pulp Capping Agents on Mesenchymal Stem Cells Isolated from Human Exfoliated Deciduous Teeth. سامانه مدیریت نشریات علمی, 1403; 25(4): 342-348. doi: 10.30476/dentjods.2023.99173.2131

Cytotoxic Effects of Pulp Capping Agents on Mesenchymal Stem Cells Isolated from Human Exfoliated Deciduous Teeth

Journal of Dentistry
مقاله 7، دوره 25، شماره 4 - شماره پیاپی 85، اسفند 2024، صفحه 342-348    اصل مقاله (380.5 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2023.99173.2131
نویسندگان
Bahareh Nazemi Salman1؛ Ehsan Saburi2؛ Mahtab Mohammadi Gheidari3؛ Mahya Farsadeghi4؛ Samira Basir Shabestari* 5
1Dept. of Pediatrics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.
2Dept. of Molecular Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3Postgraduated Student, Dept. of Endodontics, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.
4General Dentist, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.
5Dept. of ENT and Head and Neck, Firoozgar Hospital, School of Medicine, ENT and Head and Neck Research Center, Iran University of Medical Sciences, Tehran, Iran.
چکیده
Statement of the Problem: Success of pulpotomy of primary teeth depends on biological and cytotoxic effects of pulp capping agents. Mineral trioxide aggregate (MTA), Biodentine, calcium enriched mixture (CEM) cement, and ferric sulfate (FS) are among the commonly used pulp capping agents (PCAs) for pulpotomy, and their successful application has been previously evaluated.
Purpose: This study aimed to compare the cytotoxicity of PCAs against mesenchymal stem cells isolated from human exfoliated deciduous teeth (SHEDs).
Materials and Method: In this in vitro study, SHEDs were exposed to MTA, Biodentine, CEM cement, and FS for 24 and 72 hours. The methyl thiazolyl tetrazolium (MTT) assay was performed for five different concentrations of PCAs after 24 and 72 hours of exposure. Data were analyzed by ANOVA.
Results: Generally, the biocompatibility increased by reduction in concentration. All tested concentrations showed higher biocompatibility at 72 hours compared with 24 hours (p< 0.0001). Comparison of cytotoxicity of different biomaterials revealed no significant difference at any time point (p> 0.05).
Conclusion: In general, the cytotoxicity of MTA, Biodentine, CEM cement, and FS was comparable, with no significant difference. Cytotoxicity decreased over time and by a reduction in concentration of biomaterials. MTA and Biodentine showed maximum biocompatibility followed by FS, and CEM cement. 
کلیدواژه‌ها
Dental pulp capping؛ Deciduous teeth؛ Stem Cells؛ Tooth
سایر فایل های مرتبط با مقاله
مراجع
  • Nayak UA, Wadhwa S, Kashyap N, Prajapati D, Mahuli AV, Sharma R. Knowledge and practice of and attitudes toward, pulp therapy in deciduous teeth among pediatric dentists in India. J Investig Clin Dent. 2018; 9: 1.
  • Collado-González M, García-Bernal D, Oñate-Sánchez RE, Ortolani-Seltenerich PS, Álvarez-Muro T, Lozano A, et al. Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth. Int Endod J. 2017; 50 Suppl 2: e19-e30.
  • Parisay I, Ghoddusi J, Forghani M. A review on vital pulp therapy in primary teeth. Iran Endod J. 2015; 10: 6-15.
  • Laurent P, Camps J, About I. Biodentine (TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization. Int Endod J. 2012; 45: 439-448.
  • Wang J, Wang X, Sun Z, Wang X, Yang H, Shi S, et al. Stem cells from human-exfoliated deciduous teeth can differentiate into dopaminergic neuron-like cells. Stem Cells Dev. 2010; 19: 1375-1383.
  • Silva EJ, Neves AA, De-Deus G, Accorsi-Mendonça T, Moraes AP, Valentim RM, et al. Cytotoxicity and gelatinolytic activity of a new silicon-based endodontic sealer. J Appl Biomater Funct Mater. 2015; 13: e376-e380.
  • Ibrahim MS, El-Wassefy NA, Farahat DS. Biocompatibility of dental biomaterials: Biomaterials for oral and dental tissue engineering. 3th ed. Woodhead Publishing: Sawston, UK; p. 117-140.
  • Tibau A, Grube BD. Biocompatibility in Dentistry: A Mini Review. Mod Res Dent. 2021; 6: 640-643.
  • Rajasekharan S, Martens LC, Cauwels RG, Verbeeck RM. Biodentine™ material characteristics and clinical applications: a review of the literature. Eur Arch Paediatr Dent. 2014; 15: 147-158.
  • Sobhnamayan F, Adl A, Shojaee NS, Zarei Z, Emkani A. Physical and chemical properties of CEM cement mixed with propylene glycol. Iran Endod J. 2017; 12: 474.
  • Musale PK, Kulkarni N. An in vivo evaluation of biodentine ™ as a pulpotomy agent in primary teeth. J South Asian Assoc Pediatr Dent. 2018; 1: 39-46.
  • Lima RV, Esmeraldo MR, de*Carvalho MG, de*Oliveira PT, de*Carvalho RA, da*Silva FL*Jr, et al. Pulp repair after pulpotomy using different pulp capping agents: a comparative histologic analysis. Pediatr Dent. 2011; 33: 14-8.
  • Saberi E, Farhadmollashahi N, Ghotbi F, Karkeabadi H, Havaei R. Cytotoxic effects of mineral trioxide aggregate, calcium enrichedmixture cement, Biodentine and octacalcium pohosphate onhuman gingival fibroblasts. J Dent Res Dent Clin Dent Prospects. 2016; 10: 75-80.
  • Ajay Sharma L, Sharma A, Dias GJ. Advances in regeneration of dental pulp--a literature review. J Investig Clin Dent. 2015; 6: 85-98.
  • Dou L, Yan Q, Yang D. Effect of five dental pulp capping agents on cell proliferation, viability, apoptosis and mineralization of human dental pulp cells. Exp Ther Med. 2020; 19: 2377-2383.
  • Van*Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity ass-ays: the MTT assay. Method Mol Biol. 2011; 731: 237-245.
  • Nabavizadeh MR, Moazzami F, Gholami A, Mehrabi V, Ghahramani Y. Cytotoxic effect of nano fast cement and ProRoot mineral trioxide aggregate on L-929 fibroblast cells: an in vitro J Dent Shiraz Univ Med Sci. 2022; 23: 13-19.
  • Omidi S, Dadgar K, Mesgarani A, Moosazade M, Zaghi*Hosseinzadeh F. Evaluation of cytotoxicity of endoseal MTA sealer as a root canal perforation restorative material on gingival fibroblasts compared to Iranian ProRoot MTA and CEM by MTT test. J Mashhad Dent Sch. 2022; 46: 305-315.
  • Manaspon C, Jongwannasiri C, Chumprasert S. Human dental pulp stem cell responses to different dental pulp capping materials. BMC Oral Health. 2021; 21: 209.
  • Jaberiansari Z, Naderi S, Tabatabaei F. Cytotoxic effects of various mineral trioxide aggregate formulations, calciumEnriched mixture and a new cement on human pulp stem cells. Iran Endod J. 2014; 9: 271-276.
  • Zakerzadeh A, Esnaashari E, Dadfar S. In vitro comparison of cytotoxicity and genotoxicity of three vital pulp capping materials. Iran Endod J. 2017; 12: 419-425.
  • Moshary A, Almasi M, Bitaraf T. Evaluation of cytotoxicity of ProRoot MTA and endocem at different times and concentrations on human gingival fibroblasts. J Res Dent Maxillofac Sci. 2021; 6: 1-7.
  • Nowakowska D, Saczko J, Kulbacka J, Choromanska A. Dynamic oxidoreductive potential of astringent retraction agents. Folia Biol (Praha).2010; 56: 263-268.
  • Omidi S, Bagheri M, Fazli M, Ahmadiankia N. The effect of different pulp-capping materials on proliferation, migration and cytokine secretion of human dental pulp stem cells. Iran J Basic Med Sci. 2020; 23: 768-775.
  • Margunato S, Taşlı PN, Aydın S, Karapınar*Kazandağ M, Şahin F. In vitro evaluation of ProRoot MTA, biodentine, and MM-MTA on human alveolar bone marrow stem cells in terms of biocompatibility and mineralization. J Endod. 2015; 41: 1646-1652.
  • Adıgüzel M, Ahmetoğlu F, Eldeniz AÜ, Tekin MG, Göğ-ebakan B. Comparison of cytotoxic effects of calcium silicate-based materials on human pulp fibroblasts Mehmet. J Dent Res Dent Clin Dent Prospects. 2019; 13: 241-246.
  • Birant S, Gokalp M, Duran Y, Koruyucu M, Akkoc T, Seymen F. Cytotoxicity of NeoMTA Plus, ProRoot MTA and Biodentine on human dental pulp stem cells. J Dent Sci. 2021; 16: 971-979.
  • Dahake PT, Panpaliya NP, Kale YJ, Dadpe MV, Kendre SB, Bogar C. Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials: An in vitro experimental study. Saudi Dent J. 2020; 32: 43-51.
  • Manaspon C, Jongwannasiri C, Chumprasert S, Sa-ArdIam N, Mahanonda R, Pavasant P, et al. Human dental pulp stem cell responses to different dental pulp capping materials. BMC Oral Health. 2021; 21: 209.
آمار
تعداد مشاهده مقاله: 394
تعداد دریافت فایل اصل مقاله: 479


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب