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Abdulghany, Zaynab. (1403). Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment. سامانه مدیریت نشریات علمی, 15(2), 98-107. doi: 10.30476/mejc.2023.98580.1905
Zaynab Saad Abdulghany. "Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment". سامانه مدیریت نشریات علمی, 15, 2, 1403, 98-107. doi: 10.30476/mejc.2023.98580.1905
Abdulghany, Zaynab. (1403). 'Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment', سامانه مدیریت نشریات علمی, 15(2), pp. 98-107. doi: 10.30476/mejc.2023.98580.1905
Abdulghany, Zaynab. Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment. سامانه مدیریت نشریات علمی, 1403; 15(2): 98-107. doi: 10.30476/mejc.2023.98580.1905

Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment

Middle East Journal of Cancer
مقاله 3، دوره 15، شماره 2 - شماره پیاپی 58، تیر 2024، صفحه 98-107    اصل مقاله (2.6 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2023.98580.1905
نویسنده
Zaynab Saad Abdulghany*
Department of Molecular Biology, Iraqi Center for Cancer and Medical Genetics Research, Mustansiriyah University, Baghdad, Iraq
چکیده
Background: Glioblastoma (GBM) stands out as the most prevalent primary brain tumor characterized by its high aggressiveness. Numerous therapeutic approaches have been employed, and the utility of combination therapies has been substantiated, particularly in GBM treatment. Cisplatin, an anticancer chemotherapeutic agent, is employed for the management of various malignancies, including GBM; however, it is associated with significant systemic toxicity. In the realm of combination therapy, metformin, a biguanide drug conventionally used as a first-line treatment for type 2 diabetes, has recently emerged as a valuable adjunct in the treatment of a diverse spectrum of tumors. This study aimed to elucidate the impact of metformin on sensitizing the human cerebral GBM cancer cell line (AMGM) to cisplatin chemotherapy by employing the comet assay as a means to assess DNA damage, thereby advocating the potential of metformin as an adjuvant for cisplatin-based therapy.
Method: In this experimental study, the AMGM cell line was cultured and subsequently treated with either single-agent cisplatin, metformin, or a combination of both drugs. Cell viability was assessed through growth inhibition calculations. The Chou–Talalay analysis was used to assess the cooperative effect of this drug combination. Furthermore, DNA fragmentation was quantified using the alkaline comet assay technique.
Results: The findings demonstrate that metformin significantly potentiates the therapeutic efficacy of cisplatin by synergistically inhibiting the growth of AMGM cells and reducing DNA damage.
Conclusion: These results underscore the potential utility of metformin as a valuable adjunct in enhancing the clinical effectiveness of chemotherapy regimens.

تازه های تحقیق

Zaynab Abdulghany (Google Scholar)

کلیدواژه‌ها
Metformin؛ Cisplatin؛ Glioblastoma؛ Synergism effect؛ DNA fragmentation
مراجع
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