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Wang, Xixi. (1402). Human Umbilical Cord Mesenchymal Stem Cells and their Extracellular Vesicles Modulate Pro- and Anti-inflammatory Cytokines in Ligature-induced Periodontitis. سامانه مدیریت نشریات علمی, 20(4), 446-455. doi: 10.22034/iji.2023.100211.2683
Xixi Wang. "Human Umbilical Cord Mesenchymal Stem Cells and their Extracellular Vesicles Modulate Pro- and Anti-inflammatory Cytokines in Ligature-induced Periodontitis". سامانه مدیریت نشریات علمی, 20, 4, 1402, 446-455. doi: 10.22034/iji.2023.100211.2683
Wang, Xixi. (1402). 'Human Umbilical Cord Mesenchymal Stem Cells and their Extracellular Vesicles Modulate Pro- and Anti-inflammatory Cytokines in Ligature-induced Periodontitis', سامانه مدیریت نشریات علمی, 20(4), pp. 446-455. doi: 10.22034/iji.2023.100211.2683
Wang, Xixi. Human Umbilical Cord Mesenchymal Stem Cells and their Extracellular Vesicles Modulate Pro- and Anti-inflammatory Cytokines in Ligature-induced Periodontitis. سامانه مدیریت نشریات علمی, 1402; 20(4): 446-455. doi: 10.22034/iji.2023.100211.2683

Human Umbilical Cord Mesenchymal Stem Cells and their Extracellular Vesicles Modulate Pro- and Anti-inflammatory Cytokines in Ligature-induced Periodontitis

Iranian Journal of Immunology
مقاله 6، دوره 20، شماره 4، اسفند 2023، صفحه 446-455    اصل مقاله (1.76 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.100211.2683
نویسنده
Xixi Wang*
Department of Orthodontics, Affiliated Hospital of Tangshan Vocational and Technical College, Tangshan, Hebei Province, 630000, China.
چکیده
Background: Periodontitis is a chronic inflammatory condition that affects the tissues supporting the teeth, ultimately leading to tooth loss. Mesenchymal stem cells (MSCs) and their extracellular vesicles (EVs) play a crucial role in periodontitis by modulating the activities of gum cells and the immune system.
Objective: To investigate the therapeutic potential of human umbilical cord mesenchymal stem cells (hUCSCs) and EVs in regulating the inflammatory response associated with periodontitis.
Methods: hUCSCs were isolated, subjected to flow cytometry analysis of surface markers, and differentiated into adipocyte and osteocyte. hUCSC-EVs were isolated and characterized using flow cytometry and electron microscopy. A periodontitis animal model was established in 30 female C57Bl/6 mice. Experimental groups received hUCSCs or hUCSCs-EVs, or vehicles intravenously. Animals were monitored for 4 weeks, and the periodontal tissues were used to assess the effects of hUCSCs and hUCSCs-EVs on the expression of pro- (TNF-α, IFN-γ, and IL-17a) and anti-inflammatory cytokines (TGF-β, IL-10, and IL-4). The secretion of these cytokines by splenocytes was also evaluated using ELISA.
Results: The levels of IL-17a, IFN-γ, and TNFα significantly reduced, while TGF-β and IL-10 significantly increased in the periodontal tissues of the hUCSC and hUCSCEVs-treated mice. The expression of TNF-α, IFN-γ, and IL-17a significantly decreased, while the production of IL-10 and TGF-β significantly increased in splenocytes from the hUCSC and EVs-treated mice.
Conclusion: hUCSCs and their EVs have the potential to attenuate the inflammatory response associated with periodontitis, possibly by downregulating pro-inflammatory cytokines and upregulating anti-inflammatory ones.
کلیدواژه‌ها
Anti-Inflammatory Cytokine؛ Extracellular Vesicles؛ Mesenchymal Stem Cells؛ Periodontitis؛ Pro-Inflammatory Cytokine
مراجع
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