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Mohammadi, Maryam, Asgarian-Omran, Hossein, Najafi, Behnam, Najafi, Ahmad, Valadan, Reza, Karami, Hossein, Naderisoraki, Mohammad, Alizadeforutan, Maryam, Shekarriz, Ramin, Tehrani, Mohsen. (1402). Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia. سامانه مدیریت نشریات علمی, 20(4), 438-445. doi: 10.22034/iji.2023.97902.2537
Maryam Mohammadi; Hossein Asgarian-Omran; Behnam Najafi; Ahmad Najafi; Reza Valadan; Hossein Karami; Mohammad Naderisoraki; Maryam Alizadeforutan; Ramin Shekarriz; Mohsen Tehrani. "Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia". سامانه مدیریت نشریات علمی, 20, 4, 1402, 438-445. doi: 10.22034/iji.2023.97902.2537
Mohammadi, Maryam, Asgarian-Omran, Hossein, Najafi, Behnam, Najafi, Ahmad, Valadan, Reza, Karami, Hossein, Naderisoraki, Mohammad, Alizadeforutan, Maryam, Shekarriz, Ramin, Tehrani, Mohsen. (1402). 'Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia', سامانه مدیریت نشریات علمی, 20(4), pp. 438-445. doi: 10.22034/iji.2023.97902.2537
Mohammadi, Maryam, Asgarian-Omran, Hossein, Najafi, Behnam, Najafi, Ahmad, Valadan, Reza, Karami, Hossein, Naderisoraki, Mohammad, Alizadeforutan, Maryam, Shekarriz, Ramin, Tehrani, Mohsen. Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia. سامانه مدیریت نشریات علمی, 1402; 20(4): 438-445. doi: 10.22034/iji.2023.97902.2537

Evaluation of mRNA Expressions of TOX and NR4As in CD8+ T cells in Acute Leukemia

Iranian Journal of Immunology
مقاله 5، دوره 20، شماره 4، اسفند 2023، صفحه 438-445    اصل مقاله (674.54 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.97902.2537
نویسندگان
Maryam Mohammadi1؛ Hossein Asgarian-Omran1، 2؛ Behnam Najafi1؛ Ahmad Najafi1؛ Reza Valadan3؛ Hossein Karami4؛ Mohammad Naderisoraki4؛ Maryam Alizadeforutan2، 5؛ Ramin Shekarriz2، 5؛ Mohsen Tehrani* 1، 2
1Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
2Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
3Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
4Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran.
5Department of Hematology and Oncology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran.
چکیده
Background: Thymocyte selection-associated high mobility group box protein (TOX) and members of the nuclear receptor 4A (NR4A) are known as transcription factors involved in T cell exhaustion.
Objective: To evaluate the mRNA expression of TOX and NR4A1-3 in CD8+ T cells in acute leukemia.
Methods: Blood samples were obtained from 21 ALL and 6 AML patients as well as 20 control subjects. CD8+ T cells were isolated using MACS. Relative gene expression of TOX and NR4A1-3 was then evaluated using qRT-PCR.
Results: Comparison of mRNA expression of TOX in CD8+ T cells showed no significant difference among the study groups (p>0.05), while the expression of NR4A1 was significantly lower in AML patients than in the control group (p=0.0006). Also, the expression of NR4A2 and NR4A3 was significantly lower in both ALL (p=0.0049 and p=0.0005, respectively) and AML (p=0.0019 and p=0.0055, respectively) patients.
Conclusion: NR4As expressions were found to be lower in CD8+ T cells from patients with AML and ALL compared to controls, whereas the mRNA expression of TOX showed no significant difference. Although TOX and NR4As are associated with CD8+ T cell exhaustion in solid tumors, they might play different roles in acute leukemia, which requires further investigation.
کلیدواژه‌ها
Leukemia؛ NR4A؛ T cell Exhaustion؛ TOX
مراجع
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