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Khodkar, Iman, Saki, Jasem, Arjmand, Reza, Saki, Ghasem, Khorsandi, Layasadat. (1402). Adipose-Derived Stem Cells’ Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by Leishmania Major in Mice. سامانه مدیریت نشریات علمی, 49(2), 121-129. doi: 10.30476/ijms.2023.96413.2795
Iman Khodkar; Jasem Saki; Reza Arjmand; Ghasem Saki; Layasadat Khorsandi. "Adipose-Derived Stem Cells’ Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by Leishmania Major in Mice". سامانه مدیریت نشریات علمی, 49, 2, 1402, 121-129. doi: 10.30476/ijms.2023.96413.2795
Khodkar, Iman, Saki, Jasem, Arjmand, Reza, Saki, Ghasem, Khorsandi, Layasadat. (1402). 'Adipose-Derived Stem Cells’ Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by Leishmania Major in Mice', سامانه مدیریت نشریات علمی, 49(2), pp. 121-129. doi: 10.30476/ijms.2023.96413.2795
Khodkar, Iman, Saki, Jasem, Arjmand, Reza, Saki, Ghasem, Khorsandi, Layasadat. Adipose-Derived Stem Cells’ Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by Leishmania Major in Mice. سامانه مدیریت نشریات علمی, 1402; 49(2): 121-129. doi: 10.30476/ijms.2023.96413.2795

Adipose-Derived Stem Cells’ Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by Leishmania Major in Mice

Iranian Journal of Medical Sciences
مقاله 9، دوره 49، شماره 2، اردیبهشت 2024، صفحه 121-129    اصل مقاله (1.88 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.96413.2795
نویسندگان
Iman Khodkar1؛ Jasem Saki* 1، 2؛ Reza Arjmand1؛ Ghasem Saki3، 4؛ Layasadat Khorsandi3، 4
1Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Background: Stem cell-derived secretome (SE) released into the extracellular space contributes to tissue repair. The present study aimed to investigate the impact of isolated secretome (SE) from adipose-derived mesenchymal stem cells (ASCs) on Leishmania major (L. major) lesions in BALB/c mice. 
Methods: This experimental study was conducted at Ahvaz University of Medical Sciences (Ahvaz, Iran) in 2021. Forty female BALB/c mice were infected with stationary phase promastigotes through intradermal injection in the bottom of their tail and randomly divided into four groups (n=10 per group). The mice were given SE (20 mg/mL), either alone or in combination with Glucantime (GC, 20 mg/mL/Kg), meglumine antimoniate (20 mg/mL/Kg) for the GC group, and phosphate-buffered saline (PBS) for the control group. After eight weeks, the lesion size, histopathology, the levels of Interleukin 10 (IL-10), and Interleukin 12 (IL-12) were assessed. For the comparison of values between groups, the parametric one-way ANOVA was used to assess statistical significance.
Results: At the end of the experiment, the mice that received SE had smaller lesions (4.56±0.83 mm versus 3.62±0.59 mm, P=0.092), lower levels of IL-10 (66.5±9.7 pg/mL versus 285.4±25.2 pg/mL, P<0.001), and higher levels of IL-12 (152.2±14.2 pg/mL versus 24.2±4.4 pg/mL, P<0.001) than the control. Histopathology findings revealed that mice treated with SE had a lower parasite burden in lesions and spleen than the control group. 
Conclusion: The current study demonstrated that ADSC-derived SE could protect mice infected with L. major against leishmaniasis. 

تازه های تحقیق

Iman Khodkar (Google Scholar)

Jasem Saki (Google Scholar)

کلیدواژه‌ها
Adipose-derived mesenchymal stem cells؛ Secretome؛ Leishmania؛ Wound؛ Cytokines؛ Mice
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