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Wu, Hong, Fu, Yanqi, Jiang, Yuhuan, Liu, Yali, Cheng, Zhibin, Shao, Yanting, Nie, Yijun. (1402). High-free Fatty Acid Treatment Induced Anti-inflammatory Changes in a Natural Killer (NK) Cell Line. سامانه مدیریت نشریات علمی, 20(4), 456-465. doi: 10.22034/iji.2023.99972.2670
Hong Wu; Yanqi Fu; Yuhuan Jiang; Yali Liu; Zhibin Cheng; Yanting Shao; Yijun Nie. "High-free Fatty Acid Treatment Induced Anti-inflammatory Changes in a Natural Killer (NK) Cell Line". سامانه مدیریت نشریات علمی, 20, 4, 1402, 456-465. doi: 10.22034/iji.2023.99972.2670
Wu, Hong, Fu, Yanqi, Jiang, Yuhuan, Liu, Yali, Cheng, Zhibin, Shao, Yanting, Nie, Yijun. (1402). 'High-free Fatty Acid Treatment Induced Anti-inflammatory Changes in a Natural Killer (NK) Cell Line', سامانه مدیریت نشریات علمی, 20(4), pp. 456-465. doi: 10.22034/iji.2023.99972.2670
Wu, Hong, Fu, Yanqi, Jiang, Yuhuan, Liu, Yali, Cheng, Zhibin, Shao, Yanting, Nie, Yijun. High-free Fatty Acid Treatment Induced Anti-inflammatory Changes in a Natural Killer (NK) Cell Line. سامانه مدیریت نشریات علمی, 1402; 20(4): 456-465. doi: 10.22034/iji.2023.99972.2670

High-free Fatty Acid Treatment Induced Anti-inflammatory Changes in a Natural Killer (NK) Cell Line

Iranian Journal of Immunology
مقاله 7، دوره 20، شماره 4، اسفند 2023، صفحه 456-465    اصل مقاله (2.33 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.99972.2670
نویسندگان
Hong Wu1، 2، 3؛ Yanqi Fu1، 2؛ Yuhuan Jiang4؛ Yali Liu3، 5؛ Zhibin Cheng2؛ Yanting Shao2؛ Yijun Nie* 4
1Department of Laboratory Medicine, Jiangxi Health Vocational College, Nanchang, Jiangxi 330052, China.
2School of Laboratory Medicine, Nanchang Medical College, Nanchang, Jiangxi 330052, China.
3Key Laboratory of Pharmacodynamics and Safety Evaluation, Health Commission of Jiangxi Province, Nanchang, Jiangxi 330052, China.
4Department of Clinical Laboratory, First Affiliated Hospital, Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.
5Key Laboratory of Pharmacodynamics and Quality Evaluation on Anti-Inflammatory Chinese Herbs, Jiangxi Administration of Traditional Chinese Medicine, Nanchang, Jiangxi 330052, China.
چکیده
Background: Natural killer (NK) cells play a role in the pathogenesis of various metabolic diseases related to obesity. While our initial findings have indicated a potential involvement of NK cells in the pathogenesis of type 2 diabetes mellitus, the precise mechanism underlying NK cell-mediated development of this form of diabetes remains inadequately comprehended.
Objective: To investigate the impact and the underlying mechanism of high glucose and elevated levels of free fatty acids (FFAs) on immune and inflammatory responses and oxidative stress in NK92 cells.
Methods: In this experiment, the CCK8 cytotoxicity assay was used to select the 44.4 mM and 1.5 mM concentrations of high glucose and high FFAs, respectively, to treat NK92 cells for 4 days. The concentrations of superoxide dismutase (SOD) and glutathione (GSH) were determined using a biochemical analyzer. Intracellular reactive oxygen species (ROS) levels, cytokines concentrations (TNF-α, IFN-γ, IL-6, and IL-10), and the expression levels of intracellular molecules (perforin and granzyme B) were assessed by flow cytometry.
Results: The number of NK92 cell clumps was significantly reduced in the high-FFA (HF) group. In addition, the production of ROS and levels of cytokines (TNF-α, IFN-γ, IL-6, and IL-10) significantly decreased in the HF group but showed no significant change in the high-glucose (HG) group. This observation was consistent with the expression levels of perforin and granzyme B that decreased in the HF group.
Conclusion: High FFAs induced morphological changes and serious damage to oxidative stress and inflammatory response in NK92 cells.
کلیدواژه‌ها
Cytokines؛ High FFA؛ Intracellular Molecules؛ NK92 Cells؛ Oxidative Stress
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