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Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. (1402). Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study. سامانه مدیریت نشریات علمی, 49(1), 46-56. doi: 10.30476/ijms.2023.96905.2853
Mohamed Ali Alabiad; Ibtesam Elhasadi; Sulaiman Mohammed Alnasser; Mohammed Alorini; Ahmed Baker A Alshaikh; Fatima A. Jaber; Amany Mohamed Shalaby; Walaa Samy; Ahmed Ismail Heraiz; Khalid Mohammed Mohammed Albakoush; Dina Ahmed Khairy. "Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study". سامانه مدیریت نشریات علمی, 49, 1, 1402, 46-56. doi: 10.30476/ijms.2023.96905.2853
Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. (1402). 'Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study', سامانه مدیریت نشریات علمی, 49(1), pp. 46-56. doi: 10.30476/ijms.2023.96905.2853
Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study. سامانه مدیریت نشریات علمی, 1402; 49(1): 46-56. doi: 10.30476/ijms.2023.96905.2853

Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study

Iranian Journal of Medical Sciences
مقاله 6، دوره 49، شماره 1، فروردین 2024، صفحه 46-56    اصل مقاله (7.48 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.96905.2853
نویسندگان
Mohamed Ali Alabiad* 1؛ Ibtesam Elhasadi2؛ Sulaiman Mohammed Alnasser3؛ Mohammed Alorini4؛ Ahmed Baker A Alshaikh5؛ Fatima A. Jaber6؛ Amany Mohamed Shalaby7؛ Walaa Samy8؛ Ahmed Ismail Heraiz9؛ Khalid Mohammed Mohammed Albakoush10؛ Dina Ahmed Khairy11
1Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2Department of Pathology, Faculty of Medicine, University of Benghazi, Benghazi, Libya
3Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim, University, Buraydah, Saudi Arabia
4Department of Basic Medical Sciences, Unaizah College of Medicine and Medical Sciences, Qassim University, Unaizah, Kingdom of Saudi Arabia
55. Department of Obstetrics and Gynecology, College of Medicine, Jouf University, Sakaka, Kingdom of Saudi Arabia
6Department of Biology, College of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia
7Department of Histology and Cell Biology, School of Medicine, Tanta University, Tanta, Egypt
8Department of Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
9Department of Gynecology and Obstetrics, School of Medicine, Zagazig University, Zagazig, Egypt
10Department of Anatomy and Embryology, Faculty of Medicine, Alasmarya Islamic University, Zliten, Libya
11Department of Pathology, School of Medicine, Beni-Suef University, Beni-Suef, Egypt
چکیده
Background: Letrozole, an aromatase inhibitor, has recently been introduced as the preferred treatment option for ectopic pregnancy. To date, no study has investigated the effect of letrozole alone on placental tissue. The present study aimed to evaluate the effect of different doses of letrozole on the placenta of rats and to clarify the underlying mechanism. 
Methods: Sixty pregnant female rats were equally divided into three groups, namely the control group (GI), low-dose (0.5 mg/Kg/day) letrozole group (GII), which is equivalent to the human daily dose (HED) of 5 mg, and high-dose (1 mg/Kg/day) letrozole group (GIII), equivalent to the HED of 10 mg. Letrozole was administered by oral gavage daily from day 6 to 16 of gestation. Data were analyzed using a one-way analysis of variance followed by Tukey’s post hoc test and Chi square test. P<0.05 was considered statistically significant.
Results: Compared to the GI and GII groups, high-dose letrozole significantly increased embryonic mortality with a high post-implantation loss rate (P<0.001) and significantly reduced the number of viable fetuses (P<0.001) and placental weight (P<0.001) of pregnant rats. Moreover, it significantly reduced placental estrogen receptor (ER) and progesterone receptor (PR) (P<0.001) and the expression of vascular endothelial growth factor (P<0.001), while increasing the apoptotic index of cleaved caspase-3 (P<0.001).
Conclusion: Letrozole inhibited the expression of ER and PR in rat placenta. It interrupted stimulatory vascular signals causing significant apoptosis and placental vascular dysfunction. Letrozole in an equivalent human daily dose of 10 mg caused a high post-implantation loss rate without imposing severe side effects.

تازه های تحقیق

Mohamed Ali Alabiad (Google Scholar)

کلیدواژه‌ها
Letrozole؛ Placenta؛ Receptors؛ Estrogen؛ Progesterone؛ Vascular endothelial growth factor؛ Caspase
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مراجع
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