LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,416,626
تعداد دریافت فایل اصل مقاله11,292,850
Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. (1402). Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study. سامانه مدیریت نشریات علمی, 49(1), 46-56. doi: 10.30476/ijms.2023.96905.2853
Mohamed Ali Alabiad; Ibtesam Elhasadi; Sulaiman Mohammed Alnasser; Mohammed Alorini; Ahmed Baker A Alshaikh; Fatima A. Jaber; Amany Mohamed Shalaby; Walaa Samy; Ahmed Ismail Heraiz; Khalid Mohammed Mohammed Albakoush; Dina Ahmed Khairy. "Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study". سامانه مدیریت نشریات علمی, 49, 1, 1402, 46-56. doi: 10.30476/ijms.2023.96905.2853
Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. (1402). 'Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study', سامانه مدیریت نشریات علمی, 49(1), pp. 46-56. doi: 10.30476/ijms.2023.96905.2853
Alabiad, Mohamed Ali, Elhasadi, Ibtesam, Alnasser, Sulaiman Mohammed, Alorini, Mohammed, Alshaikh, Ahmed Baker A, Jaber, Fatima A., Shalaby, Amany Mohamed, Samy, Walaa, Heraiz, Ahmed Ismail, Albakoush, Khalid Mohammed Mohammed, Khairy, Dina Ahmed. Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study. سامانه مدیریت نشریات علمی, 1402; 49(1): 46-56. doi: 10.30476/ijms.2023.96905.2853

Effect of Aromatase Inhibitor Letrozole on the Placenta of Adult Albino Rats: A Histopathological, Immunohistochemical, and Biochemical Study

Iranian Journal of Medical Sciences
مقاله 6، دوره 49، شماره 1، فروردین 2024، صفحه 46-56    اصل مقاله (7.48 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.96905.2853
نویسندگان
Mohamed Ali Alabiad* 1؛ Ibtesam Elhasadi2؛ Sulaiman Mohammed Alnasser3؛ Mohammed Alorini4؛ Ahmed Baker A Alshaikh5؛ Fatima A. Jaber6؛ Amany Mohamed Shalaby7؛ Walaa Samy8؛ Ahmed Ismail Heraiz9؛ Khalid Mohammed Mohammed Albakoush10؛ Dina Ahmed Khairy11
1Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2Department of Pathology, Faculty of Medicine, University of Benghazi, Benghazi, Libya
3Department of Pharmacology and Toxicology, Unaizah College of Pharmacy, Qassim, University, Buraydah, Saudi Arabia
4Department of Basic Medical Sciences, Unaizah College of Medicine and Medical Sciences, Qassim University, Unaizah, Kingdom of Saudi Arabia
55. Department of Obstetrics and Gynecology, College of Medicine, Jouf University, Sakaka, Kingdom of Saudi Arabia
6Department of Biology, College of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia
7Department of Histology and Cell Biology, School of Medicine, Tanta University, Tanta, Egypt
8Department of Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
9Department of Gynecology and Obstetrics, School of Medicine, Zagazig University, Zagazig, Egypt
10Department of Anatomy and Embryology, Faculty of Medicine, Alasmarya Islamic University, Zliten, Libya
11Department of Pathology, School of Medicine, Beni-Suef University, Beni-Suef, Egypt
چکیده
Background: Letrozole, an aromatase inhibitor, has recently been introduced as the preferred treatment option for ectopic pregnancy. To date, no study has investigated the effect of letrozole alone on placental tissue. The present study aimed to evaluate the effect of different doses of letrozole on the placenta of rats and to clarify the underlying mechanism. 
Methods: Sixty pregnant female rats were equally divided into three groups, namely the control group (GI), low-dose (0.5 mg/Kg/day) letrozole group (GII), which is equivalent to the human daily dose (HED) of 5 mg, and high-dose (1 mg/Kg/day) letrozole group (GIII), equivalent to the HED of 10 mg. Letrozole was administered by oral gavage daily from day 6 to 16 of gestation. Data were analyzed using a one-way analysis of variance followed by Tukey’s post hoc test and Chi square test. P<0.05 was considered statistically significant.
Results: Compared to the GI and GII groups, high-dose letrozole significantly increased embryonic mortality with a high post-implantation loss rate (P<0.001) and significantly reduced the number of viable fetuses (P<0.001) and placental weight (P<0.001) of pregnant rats. Moreover, it significantly reduced placental estrogen receptor (ER) and progesterone receptor (PR) (P<0.001) and the expression of vascular endothelial growth factor (P<0.001), while increasing the apoptotic index of cleaved caspase-3 (P<0.001).
Conclusion: Letrozole inhibited the expression of ER and PR in rat placenta. It interrupted stimulatory vascular signals causing significant apoptosis and placental vascular dysfunction. Letrozole in an equivalent human daily dose of 10 mg caused a high post-implantation loss rate without imposing severe side effects.

تازه های تحقیق

Mohamed Ali Alabiad (Google Scholar)

کلیدواژه‌ها
Letrozole؛ Placenta؛ Receptors؛ Estrogen؛ Progesterone؛ Vascular endothelial growth factor؛ Caspase
سایر فایل های مرتبط با مقاله
مراجع
  1. Practice Committee of American Society for Reproductive M. Medical treatment of ectopic pregnancy: a committee opinion. Fertil Steril. 2013;100:638-44. doi: 10.1016/j.fertnstert.2013.06.013. PubMed PMID: 23849842.
  2. Pereira N, Gerber D, Gerber RS, Lekovich JP, Elias RT, Spandorfer SD, et al. Effect of Methotrexate or Salpingectomy for Ectopic Pregnancy on Subsequent In Vitro Fertilization-Embryo Transfer Outcomes. J Minim Invasive Gynecol. 2015;22:870-6. doi: 10.1016/j.jmig.2015.04.013. PubMed PMID: 25917279.
  3. Lee VC, Gao J, Lee KF, Ng EH, Yeung WS, Ho PC. The effect of letrozole with misoprostol for medical termination of pregnancy on the expression of steroid receptors in the placenta. Hum Reprod. 2013;28:2912-9. doi: 10.1093/humrep/det345. PubMed PMID: 23980056.
  4. Tiboni GM, Marotta F, Castigliego AP, Rossi C. Impact of estrogen replacement on letrozole-induced embryopathic effects. Hum Reprod. 2009;24:2688-92. doi: 10.1093/humrep/dep277. PubMed PMID: 19638353.
  5. Nash CM, Philp L, Shah P, Murphy KE. Letrozole pretreatment prior to medical termination of pregnancy: a systematic review. Contraception. 2018;97:504-9. doi: 10.1016/j.contraception.2017.11.003. PubMed PMID: 29154779.
  6. Paltieli Y, Eibschitz I, Ziskind G, Ohel G, Silbermann M, Weichselbaum A. High progesterone levels and ciliary dysfunction--a possible cause of ectopic pregnancy. J Assist Reprod Genet. 2000;17:103-6. doi: 10.1023/a:1009465900824. PubMed PMID: 10806589; PubMed Central PMCID: PMCPMC3455158.
  7. Lam PM, Briton-Jones C, Cheung CK, Leung SW, Cheung LP, Haines C. Increased messenger RNA expression of vascular endothelial growth factor and its receptors in the implantation site of the human oviduct with ectopic gestation. Fertil Steril. 2004;82:686-90. doi: 10.1016/j.fertnstert.2003.12.052. PubMed PMID: 15374715.
  8. Wide L, Hobson B. Chromatographic studies on a chorionic gonadotropic activity in the placenta of the rat, mouse and hamster. Ups J Med Sci. 1978;83:1-6. doi: 10.3109/03009737809179104. PubMed PMID: 705968.
  9. Rezaei Z, Ghaemi M, Feizabad E, Ghavami B, Akbari Asbagh F, Davari Tanha F, et al. The Effective Role of Adding Letrozole to Methotrexate in the Management of Tubal Ectopic Pregnancies, a Randomized Clinical Trial. Iran J Pharm Res. 2021;20:378-84. doi: 10.22037/ijpr.2021.115659.15461. PubMed PMID: 35194453; PubMed Central PMCID: PMCPMC8842609.
  10. Auger N, Ayoub A, Wei SQ. Letrozole: future alternative to methotrexate for treatment of ectopic pregnancy? Fertil Steril. 2020;114:273-4. doi: 10.1016/j.fertnstert.2020.04.063. PubMed PMID: 32622661.
  11. Perez-Cano FJ, Franch A, Castellote C, Castell M. The suckling rat as a model for immunonutrition studies in early life. Clin Dev Immunol. 2012;2012:537310. doi: 10.1155/2012/537310. PubMed PMID: 22899949; PubMed Central PMCID: PMCPMC3415261.
  12. Nair AB, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 2016;7:27-31. doi: 10.4103/0976-0105.177703. PubMed PMID: 27057123; PubMed Central PMCID: PMCPMC4804402.
  13. Chowdhury M, Steinberger E. Pituitary and plasma levels of gonadotrophins in foetal and newborn male and female rats. J Endocrinol. 1976;69:381-4. doi: 10.1677/joe.0.0690381. PubMed PMID: 950526.
  14. Crowe AR, Yue W. Semi-quantitative Determination of Protein Expression using Immunohistochemistry Staining and Analysis: An Integrated Protocol. Bio Protoc. 2019;9. doi: 10.21769/BioProtoc.3465. PubMed PMID: 31867411; PubMed Central PMCID: PMCPMC6924920.
  15. Mitwally MF, Hozayen WG, Hassanin KMA, Abdalla KA, Abdalla NK. Aromatase inhibitor letrozole: a novel treatment for ectopic pregnancy. Fertil Steril. 2020;114:361-6. doi: 10.1016/j.fertnstert.2020.04.001. PubMed PMID: 32622660.
  16. Watanabe A, Yasumizu T, Hoshi K, Katoh R, Kawaoi A, Shibuya M. Vascular endothelial growth factor expression in the rat uterus and placenta throughout pregnancy. Acta histochemica et cytochemica. 1998;31:419-26. doi: 10.1267/ahc.31.419.
  17. Cabar FR, Pereira PP, Schultz R, Francisco RP, Zugaib M. Vascular endothelial growth factor and beta-human chorionic gonadotropin are associated with trophoblastic invasion into the tubal wall in ectopic pregnancy. Fertil Steril. 2010;94:1595-600. doi: 10.1016/j.fertnstert.2009.10.036. PubMed PMID: 20004374.
  18. Albrecht ED, Pepe GJ. Estrogen regulation of placental angiogenesis and fetal ovarian development during primate pregnancy. Int J Dev Biol. 2010;54:397-408. doi: 10.1387/ijdb.082758ea. PubMed PMID: 19876841; PubMed Central PMCID: PMCPMC2804030.
  19. Cali U, Cavkaytar S, Sirvan L, Danisman N. Placental apoptosis in preeclampsia, intrauterine growth retardation, and HELLP syndrome: an immunohistochemical study with caspase-3 and bcl-2. Clin Exp Obstet Gynecol. 2013;40:45-8. PubMed PMID: 23724505.
  20. Agoreyo F, Onwegbu V. Quantitative evaluation of Serum Progesterone levels in the three trimesters of pregnancy in Albino rat. Journal of Applied Sciences and Environmental Management. 2015;19:77-9. doi: 10.4314/jasem.v19i1.10.
  21. Gharia B, Seegobin K, Maharaj S, Marji N, Deutch A, Zuberi L. Letrozole-induced hepatitis with autoimmune features: a rare adverse drug reaction with review of the relevant literature. Oxf Med Case Reports. 2017;2017:omx074. doi: 10.1093/omcr/omx074. PubMed PMID: 29230302; PubMed Central PMCID: PMCPMC5691789.
  22. Wang Y, Pan P, Li X, Zhu Q, Huang T, Ge RS. Food components and environmental chemicals of inhibiting human placental aromatase. Food Chem Toxicol. 2019;128:46-53. doi: 10.1016/j.fct.2019.03.043. PubMed PMID: 30922969.
  23. Al-Bader MD. Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and Western blotting. Reprod Biol Endocrinol. 2006;4:13. doi: 10.1186/1477-7827-4-13. PubMed PMID: 16569236; PubMed Central PMCID: PMCPMC1450289.
  24. Horne AW, Shaw JL, Murdoch A, McDonald SE, Williams AR, Jabbour HN, et al. Placental growth factor: a promising diagnostic biomarker for tubal ectopic pregnancy. J Clin Endocrinol Metab. 2011;96:E104-8. doi: 10.1210/jc.2010-1403. PubMed PMID: 21047920; PubMed Central PMCID: PMCPMC3017520.
  25. Ahmed A, Dunk C, Ahmad S, Khaliq A. Regulation of placental vascular endothelial growth factor (VEGF) and placenta growth factor (PIGF) and soluble Flt-1 by oxygen--a review. Placenta. 2000;21 Suppl A:S16-24. doi: 10.1053/plac.1999.0524. PubMed PMID: 10831117.
  26. Hendriks E, Rosenberg R, Prine L. Ectopic Pregnancy: Diagnosis and Management. Am Fam Physician. 2020;101:599-606. PubMed PMID: 32412215.
  27. Mutter GL, Prat J. Pathology of the Female Reproductive Tract E-Book. Amsterdam: Elsevier Health Sciences; 2014.
  28. Javanmanesh F, Kashanian M, Mirpang S. Comparison of using misoprostol with or without letrozole in abortion induction: a placebo-controlled clinical trial. Journal of Obstetrics, Gynecology and Cancer Research. 2022;3:49-52. doi: 10.30699/jogcr.3.2.49.
آمار
تعداد مشاهده مقاله: 3,322
تعداد دریافت فایل اصل مقاله: 589


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب