LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,092
تعداد مقالات10,022
تعداد مشاهده مقاله27,184,277
تعداد دریافت فایل اصل مقاله7,457,548
Wang, Mingxia, Qiao, Fei, Li, Zihua, Wang, Qiang, Shang, Zailing, Hei, Junhu, Ma, Xuelin, Wang, Yana. (1402). Impact of Echinococcus granulosus Antigens on Monocyte Development and Dendritic Cell Differentiation. سامانه مدیریت نشریات علمی, 20(3), 348-358. doi: 10.22034/iji.2023.98163.2557
Mingxia Wang; Fei Qiao; Zihua Li; Qiang Wang; Zailing Shang; Junhu Hei; Xuelin Ma; Yana Wang. "Impact of Echinococcus granulosus Antigens on Monocyte Development and Dendritic Cell Differentiation". سامانه مدیریت نشریات علمی, 20, 3, 1402, 348-358. doi: 10.22034/iji.2023.98163.2557
Wang, Mingxia, Qiao, Fei, Li, Zihua, Wang, Qiang, Shang, Zailing, Hei, Junhu, Ma, Xuelin, Wang, Yana. (1402). 'Impact of Echinococcus granulosus Antigens on Monocyte Development and Dendritic Cell Differentiation', سامانه مدیریت نشریات علمی, 20(3), pp. 348-358. doi: 10.22034/iji.2023.98163.2557
Wang, Mingxia, Qiao, Fei, Li, Zihua, Wang, Qiang, Shang, Zailing, Hei, Junhu, Ma, Xuelin, Wang, Yana. Impact of Echinococcus granulosus Antigens on Monocyte Development and Dendritic Cell Differentiation. سامانه مدیریت نشریات علمی, 1402; 20(3): 348-358. doi: 10.22034/iji.2023.98163.2557

Impact of Echinococcus granulosus Antigens on Monocyte Development and Dendritic Cell Differentiation

Iranian Journal of Immunology
دوره 20، شماره 3، آذر 2023، صفحه 348-358    اصل مقاله (3.15 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.98163.2557
نویسندگان
Mingxia Wang1؛ Fei Qiao1؛ Zihua Li1، 2؛ Qiang Wang2؛ Zailing Shang1؛ Junhu Hei1؛ Xuelin Ma1؛ Yana Wang* 1، 2
1Basic Medical Institute of Ningxia Medical University, Yinchuan, Ningxia, China.
2Key Laboratory of Common Infectious Disease of Ningxia Hui Autonomous Region, Yinchuan, Ningxia, China.
چکیده
Background: Different subtypes of dendritic cells (DCs) can induce different types of immune responses. Our previous study found that Echinococcus granulosus (E. granulosus) antigens (Eg.ferritin, Eg.mMDH and Eg.10) stimulated DC differentiation to different subtypes and produced different immune responses.
Objective: To further understand whether Eg.ferritin, Eg.mMDH and Eg.10 affect the DC-mediated immune response by promoting the differentiation of monocytes to DCs.
Methods: Bone marrow-derived monocytes were exposed to three antigens of E. granulosus on days 0, 3, 5, and 7. The percentage of monocyte-derived DCs (moDCs), DCs subsets, and the expression of surface molecules of DCs at different time points in different groups were assessed by flow cytometry. The levels of cytokines of IL-1β, IL-4, IL-6, IL-10, IL-13, IFN-γ, TNF-α, IL-12p70, IL-18, IL-23, and IL-27 in the cell culture supernatant were detected by multi-factorial detection technology.
Results: The percentage of moDCs revealed that none of the three antigens blocked monocyte differentiation to DCs. The monocytes of 7-day-old cultures showed increased sensitivity to these antigens. The Eg.ferritin induced more mature DCs, which expressed high levels of MHC II and costimulatory molecules, and secreted Th1 cytokines. Eg10 and Eg.mMDH induced lower degrees of DC maturation, however differentiated DCs were in a semi-mature state due to low expression of MHC II and costimulatory molecules and secretion of higher Th2 and lower Th1 cytokines.
Conclusion: Eg.ferritin promotes full maturation of DCs and induces Th1 immune response, whereas Eg.10 and Eg.mMDH induce semi-mature DCs producing higher levels of Th2 cytokines.
کلیدواژه‌ها
Dendritic Cells؛ Echinococcus granulosus؛ Immune Response؛ Monocytes
مراجع
  1. Heath D, Koolaard J. Serological monitoring of protection of sheep against Echinococcus granulosus induced by the EG95 vaccine. Parasite immunology. 2012;34(1):40-4.
  2. Wang H, Li Z, Gao F, Zhao J, Zhu M, He X, et al. Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep. Veterinary research communications. 2016;40(2):73-9.
  3. Li Z, Wang Y, Wang Q, Zhao W. Echinococcus granulosus 14-3-3 protein: a potential vaccine candidate against challenge with Echinococcus granulosus in mice. Biomedical environmental sciences : BES. 2012;25(3):352-8.
  4. Carmena D, Cardona G. Echinococcosis in wild carnivorous species: epidemiology, genotypic diversity, and implications for veterinary public health. Veterinary parasitology. 2014;202:69-94.
  5. Zhang W, Zhang Z, Wu W, Shi B, Li J, Zhou X, et al. Epidemiology and control of echinococcosis in central Asia, with particular reference to the People's Republic of China. Acta tropica. 2015;141:235-43.
  6. Hashimoto D, Miller J, Merad M. Dendritic cell and macrophage heterogeneity in vivo. Immunity. 2011;35(3):323-35.
  7. Boyette L, Macedo C, Hadi K, Elinoff B, Walters J, Ramaswami B, et al. Phenotype, function, and differentiation potential of human monocyte subsets. PloS one. 2017;12(4):e0176460.
  8. Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harbor perspectives in biology. 2014;6(10):a016295.
  9. Sander J, Schmidt S, Cirovic B, McGovern N, Papantonopoulou O, Hardt A, et al. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. Immunity. 2017;47(6):1051-66.e12.
  10. Roy S, Mukhopadhyay D, Mukherjee S, Moulik S, Chatterji S, Brahme N, et al. An IL-10 dominant polarization of monocytes is a feature of Indian Visceral Leishmaniasis. Parasite immunology. 2018;40(7):e12535.
  11. Dudek A, Martin S, Garg A, Agostinis P. Immature, Semi-Mature, and Fully Mature Dendritic Cells: Toward a DC-Cancer Cells Interface That Augments Anticancer Immunity. Frontiers in immunology. 2013;4:438.
  12. Garg A, Kaczmarek A, Krysko O, Vandenabeele P, Krysko D, Agostinis P. ER stress-induced inflammation: does it aid or impede disease progression? Trends in molecular medicine. 2012;18(10):589-98.
  13. Steinman R, Idoyaga J. Features of the dendritic cell lineage. Immunological reviews. 2010;234(1):5-17.
  14. Jin Y, Wang X, Du J, Cao R, Law H, Wang J, et al. Epstein-Barr virus induces the differentiation of semi-mature dendritic cells from cord blood monocytes. Human immunology. 2014;75(4):306-16.
  15. Frick J, Grünebach F, Autenrieth I. Immunomodulation by semi-mature dendritic cells: a novel role of Toll-like receptors and interleukin-6. International journal of medical microbiology : IJMM. 2010;300(1):19-24.
  16. Wei J, Xia S, Sun H, Zhang S, Wang J, Zhao H, et al. Critical role of dendritic cell-derived IL-27 in antitumor immunity through regulating the recruitment and activation of NK and NKT cells. Journal of immunology. 2013;191(1):500-8.
  17. Harnett W. Parasite modulation of the immune response. Parasite immunology. 2005;27:357-9.
  18. Riganò R, Buttari B, Profumo E, Ortona E, Delunardo F, Margutti P, et al. Echinococcus granulosus antigen B impairs human dendritic cell differentiation and polarizes immature dendritic cell maturation towards a Th2 cell response. Infection immunity. 2007;75(4):1667-78.
  19. Wang Y, Lv S, Wang Q, Wang C, Zhu M, Ma Z, et al. Mechanisms underlying immune tolerance caused by recombinant Echinococcus granulosus antigens Eg mMDH and Eg10 in dendritic cells. PloS one. 2018;13(9):e0204868.
  20. Wang Y, Wang Q, Lv S, Zhang S. Different protein of Echinococcus granulosus stimulates dendritic induced immune response. Parasitology. 2015;142(7):879-89.
  21. Sun Z, Zhang R, Wang H, Jiang P, Zhang J, Zhang M, et al. Serum IL-10 from systemic lupus erythematosus patients suppresses the differentiation and function of monocyte-derived dendritic cells. Journal of biomedical research. 2012;26(6):456-66.
  22. Wang Y, Hu X, He F, Feng F, Wang L, Li W, et al. Lipopolysaccharide-induced maturation of bone marrow-derived dendritic cells is regulated by notch signaling through the up-regulation of CXCR4. The Journal of biological chemistry. 2009;284(23):15993-6003.
  23. Garg A, Dudek A, Agostinis P. Autophagy-dependent suppression of cancer immunogenicity and effector mechanisms of innate and adaptive immunity. Oncoimmunology. 2013;2(10):e26260.
  24. Hurdayal R, Nieuwenhuizen N, Khutlang R, Brombacher F. Leishmania majorInflammatory Dendritic Cells, Regulated by IL-4 Receptor Alpha Signaling, Control Replication, and Dissemination of in Mice. Frontiers in cellular infection microbiology. 2019;9:479.
  25. Webb D, Cai Y, Matthaei K, Foster P. Comparative roles of IL-4, IL-13, and IL-4Ralpha in dendritic cell maturation and CD4+ Th2 cell function. Journal of immunology. 2007;178(1):219-27.
  26. Behzadi P, Behzadi E, Ranjbar R. IL-12 Family Cytokines: General Characteristics, Pathogenic Microorganisms, Receptors, and Signalling Pathways. Acta microbiologica et immunologica Hungarica. 2016;63(1):1-25.
  27. Oertli M, Sundquist M, Hitzler I, Engler D, Arnold I, Reuter S, et al. DC-derived IL-18 drives Treg differentiation, murine Helicobacter pylori-specific immune tolerance, and asthma protection. The Journal of clinical investigation. 2012;122(3):1082-96.
  28. Arnold I, Mathisen S, Schulthess J, Danne C, Hegazy A, Powrie F. CD11c(+) monocyte/macrophages promote chronic Helicobacter hepaticus-induced intestinal inflammation through the production of IL-23. Mucosal immunology. 2016;9(2):352-63.
  29. Wei J, Xia S, Sun H, Zhang S, Wang J, Zhao H, et al. Critical role of dendritic cell-derived IL-27 in antitumor immunity through regulating the recruitment and activation of NK and NKT cells.The journal of immunology. 2013;191(1):500-8.
آمار
تعداد مشاهده مقاله: 850
تعداد دریافت فایل اصل مقاله: 568


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب