SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway | ||
| Iranian Journal of Immunology | ||
| دوره 20، شماره 3، پاییز 2023، صفحه 335-347 اصل مقاله (10.48 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22034/iji.2023.98236.2559 | ||
| نویسندگان | ||
| Wenfei Gu؛ GuangTao Qi؛ Li Chen* | ||
| Department of Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, China. | ||
| چکیده | ||
| Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity. Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells. Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays. Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0.05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0.05). Importantly, the JAK/STAT pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0.05). Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT pathway. | ||
| کلیدواژهها | ||
| JAK/STAT Pathway؛ Ovarian Cancer؛ PD-L1 (CD274)؛ SPIB | ||
| مراجع | ||
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