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Gu, Wenfei, Qi, GuangTao, Chen, Li. (1402). SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. سامانه مدیریت نشریات علمی, 20(3), 335-347. doi: 10.22034/iji.2023.98236.2559
Wenfei Gu; GuangTao Qi; Li Chen. "SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway". سامانه مدیریت نشریات علمی, 20, 3, 1402, 335-347. doi: 10.22034/iji.2023.98236.2559
Gu, Wenfei, Qi, GuangTao, Chen, Li. (1402). 'SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway', سامانه مدیریت نشریات علمی, 20(3), pp. 335-347. doi: 10.22034/iji.2023.98236.2559
Gu, Wenfei, Qi, GuangTao, Chen, Li. SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. سامانه مدیریت نشریات علمی, 1402; 20(3): 335-347. doi: 10.22034/iji.2023.98236.2559

SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway

Iranian Journal of Immunology
دوره 20، شماره 3، آذر 2023، صفحه 335-347    اصل مقاله (10.48 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.98236.2559
نویسندگان
Wenfei Gu؛ GuangTao Qi؛ Li Chen*
Department of Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, China.
چکیده
Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity.
Objective: To investigate the functions and mechanisms of SPIB in ovarian cancer (OC) cells.
Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays.
Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0.05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0.05). Importantly, the JAK/STAT pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0.05).
Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT pathway.
کلیدواژه‌ها
JAK/STAT Pathway؛ Ovarian Cancer؛ PD-L1 (CD274)؛ SPIB
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