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Gharabeigi, Natalie, Tabatabaee Bafroee, Akram Sadat, Amini, Kumarss. (1402). Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection. سامانه مدیریت نشریات علمی, 48(6), 542-550. doi: 10.30476/ijms.2023.96346.2787
Natalie Gharabeigi; Akram Sadat Tabatabaee Bafroee; Kumarss Amini. "Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection". سامانه مدیریت نشریات علمی, 48, 6, 1402, 542-550. doi: 10.30476/ijms.2023.96346.2787
Gharabeigi, Natalie, Tabatabaee Bafroee, Akram Sadat, Amini, Kumarss. (1402). 'Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection', سامانه مدیریت نشریات علمی, 48(6), pp. 542-550. doi: 10.30476/ijms.2023.96346.2787
Gharabeigi, Natalie, Tabatabaee Bafroee, Akram Sadat, Amini, Kumarss. Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection. سامانه مدیریت نشریات علمی, 1402; 48(6): 542-550. doi: 10.30476/ijms.2023.96346.2787

Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection

Iranian Journal of Medical Sciences
مقاله 3، دوره 48، شماره 6، بهمن و اسفند 2023، صفحه 542-550    اصل مقاله (507.69 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.96346.2787
نویسندگان
Natalie Gharabeigi1؛ Akram Sadat Tabatabaee Bafroee* 1؛ Kumarss Amini2
1Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
2Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
چکیده
Background: Group B Streptococcus (GBS) can cause serious infections in neonates and pregnant women. GBS may cause urinary tract infections (UTIs). However, molecular epidemiology of such infections is rarely reported. The present study aimed to determine drug resistance patterns and molecular serotyping of GBS isolates in a population of pregnant Iranian women with UTIs. 
Methods: A cross-sectional study was conducted during the first half of 2021 in the Department of Biology, East Tehran Branch, Islamic Azad University (Tehran, Iran). Sixty GBS strains isolated from the urine and placenta samples of pregnant women with UTIs were evaluated. The women were aged 19-46 years old at 35 to 37 weeks of gestation. The molecular serotype of GBS isolates was determined using a multiplex polymerase chain reaction, and the disc diffusion method was used to determine the antibiotic susceptibility pattern of isolates for different antibiotics. The association of the GBS serotype with the phenotype of antibiotic resistance was statistically analyzed using SPSS software (version 22.0) with a Chi square test and Cramer’s V test. P<0.05 was considered statistically significant. 
Results: GBS capsular serotype II was most prevalent (66.7%) followed by serotypes Ib (21.7%), Ia (3.3%), and III (1.7%). The prevalence of non-typeable isolates was significantly low (6.6%). Of the 60 GBS isolates, 18.3% were resistant to penicillin, 81.6% to ampicillin, 23.3% to clindamycin, and 30% to vancomycin; indicating the need for treatment alternatives. 
Conclusion: Region-specific information on antibiotic resistance and molecular characteristics of GBS is essential for epidemiological investigations, effective treatment, and vaccine development.

تازه های تحقیق

Natalie Gharabeigi (Google Scholar)

Akram Sadat Tabatabaee Bafroee (Google Scholar)

کلیدواژه‌ها
Streptococcus agalactiae؛ Pregnant women؛ Urinary tract infection؛ Serotyping؛ Drug resistance
سایر فایل های مرتبط با مقاله
مراجع
  1. Morozumi M, Chiba N, Igarashi Y, Mitsuhashi N, Wajima T, Iwata S, et al. Direct identification of Streptococcus agalactiae and capsular type by real-time PCR in vaginal swabs from pregnant women. J Infect Chemother. 2015;21:34-8. doi: 10.1016/j.jiac.2014.08.024. PubMed PMID: 25287153.
  2. Motallebirad T, Fazeli H, Ghahiri A, Shokri D, Jalalifar S, Moghim S, et al. Prevalence, population structure, distribution of serotypes, pilus islands and resistance genes among erythromycin-resistant colonizing and invasive Streptococcus agalactiae isolates recovered from pregnant and non-pregnant women in Isfahan, Iran. BMC Microbiol. 2021;21:139. doi: 10.1186/s12866-021-02186-2. PubMed PMID: 33947330; PubMed Central PMCID: PMCPMC8096152.
  3. Sadeh M, Salehi-Abargouei A, Azartoos N, Mirzaei F, Khalili MB. Distribution of Streptococcus agalactiae among Iranian women from 1992 to 2018: A systematic review and meta-analysis. Jundishapur Journal of Microbiology. 2020;13. doi: 10.5812/jjm.102314.
  4. Mohamadi J, Pakzad I, Khodai Z, Havasian MR. The prevalence of vaginal carrier of group B Streptococcal (GBS) among pregnant women attending maternity at Mustafa-Khomeini Hospital, Ilam. Journal of Experimental Biology. 2017;5:561-67. doi: 10.18006/2017.5(4).561.567.
  5. Sadeh M, Firouzi R, Derakhshandeh A, Bagher Khalili M, Kong F, Kudinha T. Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran. Jundishapur J Microbiol. 2016;9:e30412. doi: 10.5812/jjm.30412. PubMed PMID: 27127592; PubMed Central PMCID: PMCPMC4842249.
  6. Lopez Y, Parra E, Cepas V, Sanfeliu I, Juncosa T, Andreu A, et al. Serotype, virulence profile, antimicrobial resistance and macrolide-resistance determinants in Streptococcus agalactiae isolates in pregnant women and neonates in Catalonia, Spain. Enferm Infecc Microbiol Clin (Engl Ed). 2018;36:472-7. doi: 10.1016/j.eimc.2017.08.006. PubMed PMID: 29029763.
  7. Goudarzi L, bagher Khalili M, Vakili M, Sadeh M. Prevalence and serotype determination of streptococcus agalactiae isolated from non-pregnant women in Tehran, Iran. Acta Medica Iranica. 2019:560-4. doi: 10.18502/acta.v57i9.2641.
  8. Khatami A, Randis TM, Tavares L, Gegick M, Suzman E, Ratner AJ. Vaginal co-colonization with multiple Group B Streptococcus serotypes. Vaccine. 2019;37:409-11. doi: 10.1016/j.vaccine.2018.12.001. PubMed PMID: 30528847; PubMed Central PMCID: PMCPMC6321774.
  9. Haimbodi EL, Mukesi M, Moyo SR. Prevalence and molecular characterization of group B streptococcus in pregnant women from hospitals in Ohangwena and Oshikoto regions of Namibia. BMC Microbiol. 2021;21:224. doi: 10.1186/s12866-021-02283-2. PubMed PMID: 34353281; PubMed Central PMCID: PMCPMC8340439.
  10. Khademi F, Sahebkar A. Group B streptococcus drug resistance in pregnant women in Iran: a meta-analysis. Taiwan J Obstet Gynecol. 2020;59:635-42. doi: 10.1016/j.tjog.2020.07.002. PubMed PMID: 32917310.
  11. Genovese C, D’Angeli F, Di Salvatore V, Tempera G, Nicolosi D. Streptococcus agalactiae in pregnant women: serotype and antimicrobial susceptibility patterns over five years in Eastern Sicily (Italy). Eur J Clin Microbiol Infect Dis. 2020;39:2387-96. doi: 10.1007/s10096-020-03992-8. PubMed PMID: 32700131; PubMed Central PMCID: PMCPMC7669783.
  12. Gizachew M, Tiruneh M, Moges F, Tessema B. Streptococcus agalactiae maternal colonization, antibiotic resistance and serotype profiles in Africa: a meta-analysis. Ann Clin Microbiol Antimicrob. 2019;18:14. doi: 10.1186/s12941-019-0313-1. PubMed PMID: 30922308; PubMed Central PMCID: PMCPMC6437888.
  13. Poyart C, Tazi A, Reglier-Poupet H, Billoet A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol. 2007;45:1985-8. doi: 10.1128/JCM.00159-07. PubMed PMID: 17376884; PubMed Central PMCID: PMCPMC1933079.
  14. Teatero S, McGeer A, Low DE, Li A, Demczuk W, Martin I, et al. Characterization of invasive group B streptococcus strains from the greater Toronto area, Canada. J Clin Microbiol. 2014;52:1441-7. doi: 10.1128/JCM.03554-13. PubMed PMID: 24554752; PubMed Central PMCID: PMCPMC3993709.
  15. Patel J, Cockerill F, Bradford P, Eliopoulos G, Hindler J, Jenkins S, et al. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2017:42-5.
  16. Burcham LR, Spencer BL, Keeler LR, Runft DL, Patras KA, Neely MN, et al. Determinants of Group B streptococcal virulence potential amongst vaginal clinical isolates from pregnant women. PLoS One. 2019;14:e0226699. doi: 10.1371/journal.pone.0226699. PubMed PMID: 31851721; PubMed Central PMCID: PMCPMC6919605.
  17. Yonemura Y, Canbay E, Endou Y, Ishibashi H, Mizumoto A, Li Y. A new bidirectional intraperitoneal and systemic induction chemotherapy (BISIC) for the peritoneal metastasis from gastric cancer in neoadjuvant setting. Integr Cancer Sci Ther Integr Cancer Sci Ther. 2014;1:26-9. doi: 10.15761/ICST.1000106.
  18. Rostami S, Moeineddini L, Ghandehari F, Khorasani MR, Shoaei P, Ebrahimi N. Macrolide-resistance, capsular genotyping and associated factors of group B Streptococci colonized pregnant women in Isfahan, Iran. Iran J Microbiol. 2021;13:183-9. doi: 10.18502/ijm.v13i2.5979. PubMed PMID: 34540153; PubMed Central PMCID: PMCPMC8408030.
  19. Rahnama S, Forouhesh Tehrani H, Amirmozafari N, Azadmanesh K, Biglari S. Distribution of capsular serotypes in group b streptococci clinical. Razi Journal of Medical Sciences. 2010;17:25-33.
  20. Arabestani MR, Mousavi SM, Nasaj M. Genotyping of clinical Streptococcus agalactiae strains based on molecular serotype of capsular (cps) gene cluster sequences using polymerase chain reaction. Archives of Clinical Infectious Diseases. 2017;12. doi: 10.5812/archcid.36787.
  21. Minarovits J. Microbe-induced epigenetic alterations in host cells: the coming era of patho-epigenetics of microbial infections. A review. Acta Microbiol Immunol Hung. 2009;56:1-19. doi: 10.1556/AMicr.56.2009.1.1. PubMed PMID: 19388554.
  22. Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P. Maternal carriage and neonatal colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iranian Journal of Medical Sciences. 2007;32:177-81.
  23. Kimura K, Matsubara K, Yamamoto G, Shibayama K, Arakawa Y. Active screening of group B streptococci with reduced penicillin susceptibility and altered serotype distribution isolated from pregnant women in Kobe, Japan. Jpn J Infect Dis. 2013;66:158-60. doi: 10.7883/yoken.66.158. PubMed PMID: 23514916.
  24. Eskandarian N, Ismail Z, Neela V, van Belkum A, Desa MN, Amin Nordin S. Antimicrobial susceptibility profiles, serotype distribution and virulence determinants among invasive, non-invasive and colonizing Streptococcus agalactiae (group B streptococcus) from Malaysian patients. Eur J Clin Microbiol Infect Dis. 2015;34:579-84. doi: 10.1007/s10096-014-2265-x. PubMed PMID: 25359580; PubMed Central PMCID: PMCPMC4356882.
  25. Shabayek S, Abdalla S, Abouzeid AM. Serotype and surface protein gene distribution of colonizing group B streptococcus in women in Egypt. Epidemiol Infect. 2014;142:208-10. doi: 10.1017/S0950268813000848. PubMed PMID: 23561305; PubMed Central PMCID: PMCPMC9152610.
  26. Slotved HC, Dayie N, Banini JAN, Frimodt-Moller N. Carriage and serotype distribution of Streptococcus agalactiae in third trimester pregnancy in southern Ghana. BMC Pregnancy Childbirth. 2017;17:238. doi: 10.1186/s12884-017-1419-0. PubMed PMID: 28732495; PubMed Central PMCID: PMCPMC5520380.
  27. Ulett KB, Benjamin WH, Jr., Zhuo F, Xiao M, Kong F, Gilbert GL, et al. Diversity of group B streptococcus serotypes causing urinary tract infection in adults. J Clin Microbiol. 2009;47:2055-60. doi: 10.1128/JCM.00154-09. PubMed PMID: 19439533; PubMed Central PMCID: PMCPMC2708523.
  28. Schuab RB, Areas GP, Souza VC, Barros RR. Molecular epidemiology of Streptococcus agalactiae recovered from significant bacteriuria. Infect Dis (Lond). 2015;47:637-42. doi: 10.3109/23744235.2015.1040446. PubMed PMID: 25901729.
  29. Magliano E, Grazioli V, Deflorio L, Leuci AI, Mattina R, Romano P, et al. Gender and age-dependent etiology of community-acquired urinary tract infections. Scientific World Journal. 2012;2012:349597. doi: 10.1100/2012/349597. PubMed PMID: 22629135; PubMed Central PMCID: PMCPMC3351074.
  30. Anthony BF, Concepcion NF. Group B streptococcus in a general hospital. J Infect Dis. 1975;132:561-7. doi: 10.1093/infdis/132.5.561. PubMed PMID: 1102616.
  31. Moroi H, Kimura K, Kotani T, Tsuda H, Banno H, Jin W, et al. Isolation of group B Streptococcus with reduced beta-lactam susceptibility from pregnant women. Emerg Microbes Infect. 2019;8:2-7. doi: 10.1080/22221751.2018.1557987. PubMed PMID: 30866792; PubMed Central PMCID: PMCPMC6455180.
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