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Daneshpazhouh, Hamed, Hayati Roodbari, Nasim, Tahamtani, Yaser, Khodabandeh, Zahra, Dianatpour, Mehdi. (1402). Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes. سامانه مدیریت نشریات علمی, 48(5), 501-509. doi: 10.30476/ijms.2023.88390.2811
Hamed Daneshpazhouh; Nasim Hayati Roodbari; Yaser Tahamtani; Zahra Khodabandeh; Mehdi Dianatpour. "Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes". سامانه مدیریت نشریات علمی, 48, 5, 1402, 501-509. doi: 10.30476/ijms.2023.88390.2811
Daneshpazhouh, Hamed, Hayati Roodbari, Nasim, Tahamtani, Yaser, Khodabandeh, Zahra, Dianatpour, Mehdi. (1402). 'Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes', سامانه مدیریت نشریات علمی, 48(5), pp. 501-509. doi: 10.30476/ijms.2023.88390.2811
Daneshpazhouh, Hamed, Hayati Roodbari, Nasim, Tahamtani, Yaser, Khodabandeh, Zahra, Dianatpour, Mehdi. Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes. سامانه مدیریت نشریات علمی, 1402; 48(5): 501-509. doi: 10.30476/ijms.2023.88390.2811

Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes

Iranian Journal of Medical Sciences
مقاله 8، دوره 48، شماره 5، آذر 2023، صفحه 501-509    اصل مقاله (1.4 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.88390.2811
نویسندگان
Hamed Daneshpazhouh1؛ Nasim Hayati Roodbari1؛ Yaser Tahamtani2؛ Zahra Khodabandeh3؛ Mehdi Dianatpour* 1، 3
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
3Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Autophagy is a conservative mechanism for cell survival as the main response of cells to stress conditions. The present study aimed to assess the effect of docetaxel on the survival, fertilization, and expression of autophagy-related genes in vitrified oocytes. 
Methods: The study was conducted in 2018 at the Stem Cells Technology Research Center, Shiraz University of Medical Sciences (Shiraz, Iran). Denuded oocytes were randomly selected and assigned to five groups, namely control (n=133), docetaxel (n=136), docetaxel+cryoprotectants (n=146), docetaxel+vitrification (n=138), and vitrification (n=145). The effect of vitrification on the expression of autophagy-related gene 5 (ATG5) and Beclin-1 was determined using a real-time polymerase chain reaction. Data were analyzed using SPSS software (version 26.0) and GraphPad Prism 9.
Results: Survival and fertilization rates in each experimental group were significantly reduced compared to the control group (P=0.001). After in vitro fertilization of oocytes, the 2-cell formation rate was significantly reduced in the docetaxel+vitrification and vitrification groups compared to the control and docetaxel groups (P=0.001 and P=0.001, respectively). Pre-incubation of oocytes with docetaxel reduced gene expression levels of Beclin-1 and ATG5 in the docetaxel+cryoprotectants and docetaxel+vitrification groups (P=0.001 and P=0.019, respectively). The expression level of these genes was also reduced in the docetaxel group compared to the control group (P=0.001). 
Conclusion: Incubation of mouse metaphase II oocytes with docetaxel prior to vitrification reduced the expression of autophagy-related genes and increased survival and fertilization rates compared to untreated oocytes.

تازه های تحقیق

Hamed Daneshpazhouh (Google Scholar)

Mehdi Dianatpour (Google Scholar)

کلیدواژه‌ها
Oocytes؛ Vitrification؛ Docetaxel؛ Autophagy؛ Beclin-1؛ Atg5
سایر فایل های مرتبط با مقاله
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