LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,416,051
تعداد دریافت فایل اصل مقاله11,291,719
Jahanshahiafshar, Zahra, Ghorbani, Hakimeh, Seyedmajidi, Maryam, Nabahati, Mehrdad, Ebrahimnejad Gorji, Kourosh, Seyedmajidi, Seyedali, Moudi, Ehsan. (1402). Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells. سامانه مدیریت نشریات علمی, 48(6), 572-581. doi: 10.30476/ijms.2023.96522.2807
Zahra Jahanshahiafshar; Hakimeh Ghorbani; Maryam Seyedmajidi; Mehrdad Nabahati; Kourosh Ebrahimnejad Gorji; Seyedali Seyedmajidi; Ehsan Moudi. "Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells". سامانه مدیریت نشریات علمی, 48, 6, 1402, 572-581. doi: 10.30476/ijms.2023.96522.2807
Jahanshahiafshar, Zahra, Ghorbani, Hakimeh, Seyedmajidi, Maryam, Nabahati, Mehrdad, Ebrahimnejad Gorji, Kourosh, Seyedmajidi, Seyedali, Moudi, Ehsan. (1402). 'Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells', سامانه مدیریت نشریات علمی, 48(6), pp. 572-581. doi: 10.30476/ijms.2023.96522.2807
Jahanshahiafshar, Zahra, Ghorbani, Hakimeh, Seyedmajidi, Maryam, Nabahati, Mehrdad, Ebrahimnejad Gorji, Kourosh, Seyedmajidi, Seyedali, Moudi, Ehsan. Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells. سامانه مدیریت نشریات علمی, 1402; 48(6): 572-581. doi: 10.30476/ijms.2023.96522.2807

Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells

Iranian Journal of Medical Sciences
مقاله 6، دوره 48، شماره 6، بهمن و اسفند 2023، صفحه 572-581    اصل مقاله (1.37 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2023.96522.2807
نویسندگان
Zahra Jahanshahiafshar1؛ Hakimeh Ghorbani2؛ Maryam Seyedmajidi3؛ Mehrdad Nabahati4؛ Kourosh Ebrahimnejad Gorji5؛ Seyedali Seyedmajidi3؛ Ehsan Moudi* 2
1Student Research Committee, Babol University of Medical Sciences, Babol, Iran
2Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
3Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4Department of Radiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
5Department of Medical Physics, Radiobiology and Radiation Protection, School of Medicine, Babol University of Medical Sciences, Babol, Iran
چکیده
Background: Cone beam computed tomography (CBCT) and multidetector computed tomography (MDCT) are frequently used in dental and maxillofacial problems. This study aimed to assess the genotoxicity and cytotoxicity effects of CBCT and MDCT radiographies on exfoliated buccal epithelial cells during dental examinations. 
Methods: This prospective experimental study was conducted at Babol University of Medical Sciences (Babol, Iran) from March 2021 to April 2021. Buccal mucosa smears were collected bilaterally pre-exposure and 12 days after CBCT or MDCT examinations. To compare the frequency of micronuclei and other cytotoxic cellular changes such as pyknosis, karyolysis, and karyorrhexis, the paired sample t test and Wilcoxon test were used. In addition, independent sample t test, Mann-Whitney, and Chi square tests were used to investigate the differences between the imaging methods and between men and women. All statistical analyses were performed using the SPSS software, and P≤0.05 was considered statistically significant. 
Results: The current study included 60 adult patients (30 patients in each group), ranging in age from 21 to 50 years. The micronuclei and the other cytotoxic cellular changes increased significantly after CBCT and MDCT radiographic examinations on the 12th day compared to the pre-exposure results (P<0.001). MDCT had statistically higher cytotoxic and genotoxic effects than CBCT (9.4%, 23.1%, and 40% higher values in micronucleus frequency, the mean frequency of micronuclei, and other cytotoxic changes, respectively). There were no significant differences between men and women in the two examination methods (P=0.46 and P=0.49, respectively). 
Conclusion: Dental examinations with CBCT and MDCT can increase cytotoxicity and chromosomal damage in both men and women. Due to its lower radiation toxicities, CBCT can be recommended as an alternative to MDCT for dental examinations.

تازه های تحقیق

Zahra Jahanshahiafshar (Google Scholar)

Ehsan Moudi (Google Scholar)

کلیدواژه‌ها
Cone beam computed tomography؛ Multidetector computed tomography؛ DNA damage؛ Mouth mucosa
سایر فایل های مرتبط با مقاله
مراجع
  1. Agarwal P, Vinuth DP, Haranal S, Thippanna CK, Naresh N, Moger G. Genotoxic and cytotoxic effects of X-ray on buccal epithelial cells following panoramic radiography: A pediatric study. J Cytol. 2015;32:102-6. doi: 10.4103/0970-9371.160559. PubMed PMID: 26229246; PubMed Central PMCID: PMCPMC4520040.
  2. Tsapaki V. Radiation protection in dental radiology - Recent advances and future directions. Phys Med. 2017;44:222-6. doi: 10.1016/j.ejmp.2017.07.018. PubMed PMID: 28789933.
  3. Scarfe WC, Azevedo B, Toghyani S, Farman AG. Cone Beam Computed Tomographic imaging in orthodontics. Aust Dent J. 2017;62:33-50. doi: 10.1111/adj.12479. PubMed PMID: 28297089.
  4. Ferreira LM, Nejaim Y, Freitas DQ, Tabchoury CPM. The fundaments of cbct and its use for evaluation of paranasal sinuses: review of literature. Brazilian Journal of Oral Sciences. 2018;17:e18038. doi: 10.20396/bjos.v17i0.8652646.
  5. Moshfeghi M, Abedian B, Ghazizadeh Ahsaie M, Tajdini F. Prevalence of Nasal Septum Deviation Using Cone-Beam Computed Tomography: A Cross-Sectional Study. Contemp Clin Dent. 2020;11:223-8. doi: 10.4103/ccd.ccd_110_19. PubMed PMID: 33776347; PubMed Central PMCID: PMCPMC7989757.
  6. Li G, Yang P, Hao S, Hu W, Liang C, Zou BS, et al. Buccal mucosa cell damage in individuals following dental X-ray examinations. Sci Rep. 2018;8:2509. doi: 10.1038/s41598-018-20964-3. PubMed PMID: 29410514; PubMed Central PMCID: PMCPMC5802780.
  7. Chauhan V, Wilkins RC. A comprehensive review of the literature on the biological effects from dental X-ray exposures. Int J Radiat Biol. 2019;95:107-19. doi: 10.1080/09553002.2019.1547436. PubMed PMID: 30496029.
  8. Zamani H, Falahati F, Omidi R, Abedi-Firouzjah R, Zare M, Momeni F. Estimating and comparing the radiation cancer risk from cone-beam computed tomography and panoramic radiography in pediatric and adult patients. International Journal of Radiation Research. 2020;18:885-93. doi: 10.18869/acadpub.ijrr.18.4.885.
  9. Kadesjo N, Lynds R, Nilsson M, Shi XQ. Radiation dose from X-ray examinations of impacted canines: cone beam CT vs two-dimensional imaging. Dentomaxillofac Radiol. 2018;47:20170305. doi: 10.1259/dmfr.20170305. PubMed PMID: 29303367; PubMed Central PMCID: PMCPMC6047634.
  10. Nardi C, Talamonti C, Pallotta S, Saletti P, Calistri L, Cordopatri C, et al. Head and neck effective dose and quantitative assessment of image quality: a study to compare cone beam CT and multislice spiral CT. Dentomaxillofac Radiol. 2017;46:20170030. doi: 10.1259/dmfr.20170030. PubMed PMID: 28511564; PubMed Central PMCID: PMCPMC5988185.
  11. Stratis A, Zhang G, Lopez-Rendon X, Politis C, Hermans R, Jacobs R, et al. Two examples of indication specific radiation dose calculations in dental CBCT and Multidetector CT scanners. Phys Med. 2017;41:71-7. doi: 10.1016/j.ejmp.2017.03.027. PubMed PMID: 28392311.
  12. Pauwels R, Araki K, Siewerdsen JH, Thongvigitmanee SS. Technical aspects of dental CBCT: state of the art. Dentomaxillofac Radiol. 2015;44:20140224. doi: 10.1259/dmfr.20140224. PubMed PMID: 25263643; PubMed Central PMCID: PMCPMC4277439.
  13. Belmans N, Oenning AC, Salmon B, Baselet B, Tabury K, Lucas S, et al. Radiobiological risks following dentomaxillofacial imaging: should we be concerned? Dentomaxillofac Radiol. 2021;50:20210153. doi: 10.1259/dmfr.20210153. PubMed PMID: 33989056; PubMed Central PMCID: PMCPMC8404518.
  14. Altoukhi DH, Alaki S, El Ashiry E, Nassif O, Sabbahi D. Genotoxicity and cytotoxicity of cone beam computed tomography in children. BMC Oral Health. 2021;21:427. doi: 10.1186/s12903-021-01792-w. PubMed PMID: 34481467; PubMed Central PMCID: PMCPMC8418710.
  15. Basha S, Essawy M. Genotoxic and cytotoxic effects of cone beam computed tomography on exfoliated buccal epithelial cells. IOSR-JDMS. 2018;17:66-70.
  16. Ribeiro D. Evidence of genotoxicity and cytotoxicity of X-rays in the oral mucosa epithelium of adults subjected to cone beam computed tomography. Dentomaxillofac Radiol. 2019;48:20180299. doi: 10.1259/dmfr.20180299. PubMed PMID: 30285478; PubMed Central PMCID: PMCPMC6747437.
  17. Yang P, Hao S, Gong X, Li G. Cytogenetic biomonitoring in individuals exposed to cone beam CT: comparison among exfoliated buccal mucosa cells, cells of tongue and epithelial gingival cells. Dentomaxillofac Radiol. 2017;46:20160413. doi: 10.1259/dmfr.20160413. PubMed PMID: 28186847; PubMed Central PMCID: PMCPMC5595036.
  18. da Fonte JB, Andrade TM, Albuquerque RL, Jr., de Melo MFB, Takeshita WM. Evidence of genotoxicity and cytotoxicity of X-rays in the oral mucosa epithelium of adults subjected to cone beam CT. Dentomaxillofac Radiol. 2018;47:20170160. doi: 10.1259/dmfr.20170160. PubMed PMID: 29091472; PubMed Central PMCID: PMCPMC5965904.
  19. Chow SC, Shao J, Wang H, Lokhnygina Y. Sample size calculations in clinical research. Chapman and Hall/CRC. 2017:265-6. doi: 10.1080/24754269.2017.1398000.
  20. Lorenzoni DC, Fracalossi AC, Carlin V, Ribeiro DA, Sant’anna EF. Mutagenicity and cytotoxicity in patients submitted to ionizing radiation. Angle Orthod. 2013;83:104-9. doi: 10.2319/013112-88.1. PubMed PMID: 22656641; PubMed Central PMCID: PMCPMC8805537.
  21. Sharbatdaran M, Abbaszadeh H, Siadati S, Ranaee M, Hajian-Tilaki K, Rajabi-Moghaddam M. Assessment of oral cytological features in smokers and nonsmokers after application of toluidine blue. Diagn Cytopathol. 2017;45:513-9. doi: 10.1002/dc.23706. PubMed PMID: 28296246.
  22. Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutat Res. 1992;271:69-77. doi: 10.1016/0165-1161(92)90033-i. PubMed PMID: 1371831.
  23. Palla S, Rangdhol V, Uma A, Devy SA, Vandana S, Jeelani SA. Evaluation of Micronucleus and Associated Abnormalities in Buccal Cells Post CT Scan Exposure-An Observational Study. Journal of Clinical & Diagnostic Research. 2018;12. doi: 10.7860/JCDR/2018/28892.11546.
  24. Sommer S, Buraczewska I, Kruszewski M. Micronucleus Assay: The State of Art, and Future Directions. Int J Mol Sci. 2020;21. doi: 10.3390/ijms21041534. PubMed PMID: 32102335; PubMed Central PMCID: PMCPMC7073234.
  25. Milic M, Geric M, Nodilo M, Ranogajec-Komor M, Milkovic D, Gajski G. Application of the buccal micronucleus cytome assay on child population exposed to sinus X-ray. Eur J Radiol. 2020;129:109143. doi: 10.1016/j.ejrad.2020.109143. PubMed PMID: 32593079.
  26. Anbumeena S, Kannan A, Krithika C, Vasanthi V. Genotoxic and cytotoxic biomonitoring in patients exposed to panoramic dental radiography: Comparison between five different age groups. Journal of Indian Academy of Oral Medicine and Radiology. 2021;33:16. doi: 10.4103/jiaomr.jiaomr_124_20.
  27. Torres-Bugarin O, Zavala-Cerna MG, Nava A, Flores-Garcia A, Ramos-Ibarra ML. Potential uses, limitations, and basic procedures of micronuclei and nuclear abnormalities in buccal cells. Dis Markers. 2014;2014:956835. doi: 10.1155/2014/956835. PubMed PMID: 24778463; PubMed Central PMCID: PMCPMC3932264.
  28. Belmans N, Gilles L, Virag P, Hedesiu M, Salmon B, Baatout S, et al. Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations. Dentomaxillofac Radiol. 2019;48:20180428. doi: 10.1259/dmfr.20180428. PubMed PMID: 30912976; PubMed Central PMCID: PMCPMC6747439.
  29. Aggarwal N, Dang V, Aggarwal S, Bhateja S, Aggarwal R, Singla V. Micronuclei in the Oral Mucosa as a Measure of Genotoxic Damage in Dental Radiography: A Clinicopathologic Study. Oral & Maxillofacial Pathology Journal. 2019;10:5-10.
  30. Kaur I, Sheikh S, Pallagati S, Gupta D, Singh R, Aggarwal A, et al. Evaluation of Genotoxic Effects of Panoramic Dental Radiography on Cells of Oral Mucosa by Micronucleus Assay and Evaluation of Time Period Required by Cells of Oral Mucosa to Recover from the Genotoxic Effects. Dent. 2020;11:17-23. doi: 10.5005/jp-journals-10015-1703.
  31. Palla S, Rangdhol V, Uma AN, Devy SA, Shekar V. The Genotoxic and Cytotoxic Effects of CT Scan on Buccal Epithelial Cells. J Cytol. 2020;37:189-92. doi: 10.4103/JOC.JOC_120_19. PubMed PMID: 33776259; PubMed Central PMCID: PMCPMC7984523.
  32. Arora P, Devi P, Wazir SS. Evaluation of genotoxicity in patients subjected to panoramic radiography by micronucleus assay on epithelial cells of the oral mucosa. J Dent (Tehran). 2014;11:47-55. PubMed PMID: 24910676; PubMed Central PMCID: PMCPMC4037266.
  33. Santhosh K, Manzoor S, Sushanth A, Seralathan S, Rajasekar V, Jacob A. A Cross-sectional Study to Evaluate Nuclear Changes in Buccal Mucosa Following Panoramic Radiography. J Contemp Dent Pract. 2020;21:1258-61. PubMed PMID: 33850072.
  34. Ferraz GA, Costa Neto AdO, Cerqueira EdMM, Meireles JRC. Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities. Revista Brasileira de Geriatria e Gerontologia. 2016;19:627-34. doi: 10.1590/1809-98232016019.150155.
  35. Ghandehari M, Sadri D, Farhadi S. Micronucleus Assay in Cell Phone Users: Importance of Oral Mucosa Screening. Int J Prev Med. 2021;12:125. doi: 10.4103/ijpvm.IJPVM_489_19. PubMed PMID: 34760136; PubMed Central PMCID: PMCPMC8551792.
آمار
تعداد مشاهده مقاله: 3,332
تعداد دریافت فایل اصل مقاله: 526


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب