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Shahriari, Mahdi, Safaei, Akbar, Davoudi, Parvaneh, Hedayati, Bozorgmehr. (1402). MYCN Gene Copy Number Status Detected by FISH Method and Its Correlation with Outcome and Clinicopathological Variables in Childhood Neuroblastoma. سامانه مدیریت نشریات علمی, 15(1), 8-14. doi: 10.30476/mejc.2023.96601.1827
Mahdi Shahriari; Akbar Safaei; Parvaneh Davoudi; Bozorgmehr Hedayati. "MYCN Gene Copy Number Status Detected by FISH Method and Its Correlation with Outcome and Clinicopathological Variables in Childhood Neuroblastoma". سامانه مدیریت نشریات علمی, 15, 1, 1402, 8-14. doi: 10.30476/mejc.2023.96601.1827
Shahriari, Mahdi, Safaei, Akbar, Davoudi, Parvaneh, Hedayati, Bozorgmehr. (1402). 'MYCN Gene Copy Number Status Detected by FISH Method and Its Correlation with Outcome and Clinicopathological Variables in Childhood Neuroblastoma', سامانه مدیریت نشریات علمی, 15(1), pp. 8-14. doi: 10.30476/mejc.2023.96601.1827
Shahriari, Mahdi, Safaei, Akbar, Davoudi, Parvaneh, Hedayati, Bozorgmehr. MYCN Gene Copy Number Status Detected by FISH Method and Its Correlation with Outcome and Clinicopathological Variables in Childhood Neuroblastoma. سامانه مدیریت نشریات علمی, 1402; 15(1): 8-14. doi: 10.30476/mejc.2023.96601.1827

MYCN Gene Copy Number Status Detected by FISH Method and Its Correlation with Outcome and Clinicopathological Variables in Childhood Neuroblastoma

Middle East Journal of Cancer
مقاله 2، دوره 15، شماره 1 - شماره پیاپی 57، فروردین 2024، صفحه 8-14    اصل مقاله (654.34 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2023.96601.1827
نویسندگان
Mahdi Shahriari* 1؛ Akbar Safaei2؛ Parvaneh Davoudi2؛ Bozorgmehr Hedayati1
1Department of Pediatrics, Hematology-Oncology Branch, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Pathology, Molecular Pathology and Cytogenetic Branch, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Neuroblastoma is the most common extracranial solid tumor in children. MYCN gene amplification (MNA) is an independent prognostic factor for rapid tumor progression and poor prognosis, regardless of age and clinical stage. Gain of the MYCN gene locus on the short arm of chromosome 2 can also be found in neuroblastoma.
Method: In this retrospective descriptive analysis of genetic alterations in neuroblastoma tumor samples, both before and after standard chemotherapy, we examined the MYCN gene copy number status in 20 neuroblastic tumor samples using the fluorescence in situ hybridization (FISH) method. We also evaluated its relationship with clinical variables and tumor maturation after standard chemotherapy treatment. Additionally, we compared disease outcomes among different MYCN copy number categories.
Results: Among the tumor samples, four (25%) exhibited increased MYCN copy numbers, 20% showed MYCN amplification, and 5% displayed MYCN gain. We observed decreased survival rates in advanced stages of neuroblastoma. Furthermore, in male patients, we noted an association between increased MYCN copy number and metastatic tumors.
Conclusion: We found that increased MYCN copy number is moderately associated with an immature phenotype and correlated with lower event-free survival. However, we did not detect a statistically significant difference.

تازه های تحقیق

Mahdi Shahriari (Google Scholar)

کلیدواژه‌ها
Neuroblastoma؛ MYCN amplification؛ Chemotherapy؛ Refractory؛ Progression-free survival
مراجع
  1. Campbell K, Gastier-Foster JM, Mann M, Naranjo AH, Van Ryn C, Bagatell R, et al. Association of MYCN copy number with clinical features, tumor biology, and outcomes in neuroblastoma: A report from the Children's Oncology Group. Cancer. 2017;123(21):4224-35. doi: 10.1002/cncr.30873.
  2. Brodeur GM, Bagatell R. Mechanisms of neuroblastoma regression. Nat Rev Clin Oncol. 2014;11(12):704-13. doi: 10.1038/nrclinonc.2014.168.
  3. Ambros PF, Ambros IM, Brodeur GM, Haber M, Khan J, Nakagawara A, et al. International consensus for neuroblastoma molecular diagnostics: report from the International Neuroblastoma Risk Group (INRG) Biology Committee. Br J Cancer. 2009;100(9):1471-82. doi: 10.1038/sj.bjc.6605014.
  4. Mathew P, Valentine MB, Bowman LC, Rowe ST, Nash MB, Valentine VA, et al. Detection of MYCN gene amplification in neuroblastoma by fluorescence in situ hybridization: a pediatric oncology group study. Neoplasia. 2001;3(2):105-9. doi: 10.1038/sj.neo.7900146.
  5. Szewczyk K, Wieczorek A, Młynarski W, Janczar S, Woszczyk M, Gamrot Z, et al. Unfavorable outcome of neuroblastoma in patients with 2p gain. Front Oncol. 2019;9:1018. doi: 10.3389/fonc.2019.01018.
  6. Yoshimoto M, Caminada De Toledo SR, Monteiro Caran EM, de Seixas MT, de Martino Lee ML, de Campos Vieira Abib S, et al. MYCN gene amplification. Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors. Am J Pathol. 1999;155(5):1439-43. doi: 10.1016/S0002-9440(10)65457-0.
  7. Spitz R, Hero B, Skowron M, Ernestus K, Berthold F. MYCN-status in neuroblastoma: characteristics of tumours showing amplification, gain, and non-amplification. Eur J Cancer. 2004;40(18):2753-9. doi: 10.1016/j.ejca.2004.05.002.
  8. Santiago T, Tarek N, Boulos F, Hayes C, Jeha S, Raimondi S, et al. Correlation between MYCN gene status and MYCN protein expression in neuroblastoma: a pilot study to propose the use of MYCN immunohistochemistry in limited-resource areas. J Glob Oncol. 2019;5:1-7. doi: 10.1200/JGO.19.00135.
  9. Souzaki R, Tajiri T, Teshiba R, Higashi M, Kinoshita Y, Tanaka S, et al. The genetic and clinical significance of MYCN gain as detected by FISH in neuroblastoma. Pediatr Surg Int. 2011;27(3):231-6. doi: 10.1007/s00383-010-2781-4.
  10. Wang M, Zhou C, Cai R, Li Y, Gong L. Copy number gain of MYCN gene is a recurrent genetic aberration and favorable prognostic factor in Chinese pediatric neuroblastoma patients. Diagn Pathol. 2013;8:5. doi: 10.1186/1746-1596-8-5.
  11. Yue ZX, Huang C, Gao C, Xing TY, Liu SG, Li XJ, et al. MYCN amplification predicts poor prognosis based on interphase fluorescence in situ hybridization analysis of bone marrow cells in bone marrow metastases of neuroblastoma. Cancer Cell Int. 2017;17:43. doi: 10.1186/s12935-017-0412-z.
  12. Taylor CP, Bown NP, McGuckin AG, Lunec J, Malcolm AJ, Pearson AD, et al. Fluorescence in situ hybridization techniques for the rapid detection of genetic prognostic factors in neuroblastoma. United Kingdom Children's Cancer Study Group. Br J Cancer. 2000;83(1):40-9. doi: 10.1054/bjoc.2000.1280.
  13. Zareifar S, Shakibazad N, Zekavat OR, Bordbar M, Shahriari M. Successful treatment of refractory metastatic neuroblastoma with panobinostat in combination with chemotherapy agents and iodine-131-meta-iodobenzylguanidine therapy. J Oncol Pharm Pract. 2020;26(2):481-6. doi: 10.1177/1078155219852670.
  14. Lee YJ, Hah JO. Palliative effect of 131I –MIBG in relapsed Neuroblastoma after autologous peripheral blood stem cell transplantation. Korea J Ped. 2008;51(2):214-8. doi: 10.3348/kjp.2008.512.2.214
  15. Sait S, Modak S. Anti-GD2 immunotherapy for neuroblastoma. Expert Rev Anticancer Ther. 2017;17(10):889-904. doi: 10.1080/14737140.2017.1364995.
  16. Tang XX, Zhao H, Kung B, Kim DY, Hicks SL, Cohn SL, et al. The MYCN enigma: significance of MYCN expression in neuroblastoma. Cancer Res. 2006;66(5):2826-33. doi: 10.1158/0008-5472.CAN-05-0854.
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