|تعداد مشاهده مقاله||12,903,050|
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Chenopodium ambrosioides Linn Mitigates bone Loss in Rats with Periodontitis
|Journal of Dentistry|
|مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 01 خرداد 1402 اصل مقاله (897.89 K)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.30476/dentjods.2023.95767.1891|
|paula goes* 1؛ Diego Thiers Oliveira Carneiro2؛ Michael Douglas da Silva2؛ Karla Vanessa Pinto Vasconcelos3؛ Romero Dias3؛ Vanessa Costa2؛ Raquel Felipe Vasconcelos2؛ Gisele Barreto2؛ Bárbara Carneiro4؛ Mirna Marques5؛ Hellíada Chaves Vasconcelos6؛ Howard Lopes Ribeiro Júnior3؛ Jonas Nogueira Ferreira Maciel Gusmão2؛ Helson da Silveira2؛ Renata Leitão2؛ Gerly Anne de Castro Brito2؛ Karuza Maria Alves Pereira2؛ Delane Viana Gondim3|
|1Department of Pathology and Legal Medicine, School of Medicine, Federal University of Ceara, Fortaleza, Brazil|
|2Department of Morphology, School of Medicine, Federal University of Ceara, Fortaleza, Brazil.|
|3Department of Morphology, School of Medicine, Federal University of Ceara, Fortaleza, Brazil|
|4Post-graduation Program of Dentistry, School of Dentistry, Federal University of Ceara, Fortaleza, Brazil.|
|5Post-graduation Program in Health Science, School of Medicine, Federal University of Ceara, Sobral, Brazil|
|6Post-graduation Program in Health Science, School of Dentistry, Federal University of Ceara, Sobral, Brazil|
|Statement of the Problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis. |
Purpose: This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats.
Materials and Method: For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract.
Results: Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.
Conclusion: In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent.
|Periodontitis؛ Inflammation؛ Chenopodium ambrosioides؛ Flavonoids؛ Rutin|
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