|تعداد مشاهده مقاله||12,902,656|
|تعداد دریافت فایل اصل مقاله||6,147,547|
The Upregulation of HLA-G1 and miRNA-34a in Lens Epithelial Cells of Diabetic Retinopathy Patients
|Iranian Journal of Immunology|
|مقاله 6، دوره 20، شماره 2، شهریور 2023، صفحه 202-210 اصل مقاله (798.02 K)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.22034/iji.2023.97403.2510|
|Elnaz Taghvaei-Bijandi1؛ Fatemeh Abedian1؛ Ahmad Ahmadzadeh Amiri2؛ Narjes Jafari1؛ Saeid Abediankenari* 1|
|1Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran.|
|2Department of Ophthalmology, Mazandaran University of Medical Sciences, Sari, Iran.|
|Background: Retinopathy of diabetes is a chronic diabetes mellitus complication affecting retinal vessels, and some ocular complications’ molecular mechanisms remain obscure.|
Objective: To evaluate the expression of HLA-G1, HLA-G5, miRNA-181a, and miRNA-34a in the lens epithelial cells of patients with retinopathy of diabetes.
Methods: In a case-control study, 30 diabetic patients with retinopathy, 30 diabetic patients without retinopathy, and 30 cataract patients without diabetes mellitus as the control group were enrolled after a full description with details about the study methods and objectives. The expression of HLA G1, HLA G5, miRNA-181a, and miRNA-34a in lens epithelial cells was assessed by quantitative RT PCR. Moreover, the levels of HLA-G protein in aqueous humor were evaluated by the ELISA method.
Results: HLA-G1 expression was significantly upregulated in the retinopathy group (P=0.003). The aqueous humor of diabetic retinopathy patients contained significantly higher levels of HLA-G protein compared with the non-diabetic patients (P=0.001). miRNA-181a was significantly downregulated in the diabetic retinopathy group compared with the patients without diabetes (P=0.001). In addition, miRNA-34a was upregulated in the retinopathy group (P=0.009).
Conclusion: Taken together, the present results showed that HLA-G1 and miRNA-34a can be valuable markers for diabetic retinopathy. Our data offers new perspectives for improving the control of inflammation in the lens epithelial cells by considering HLA-G and miRNA.
|Diabetic Retinopathy؛ HLA-G؛ Lens Epithelial Cells؛ miRNA-181a؛ miRNA34a|
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