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Taha, Mohammed, Fauriat, Cyril. (1402). Improvement of NK Cell Cytotoxicity in Reconstituting NK Cells after Allogeneic Stem Cell Transplantation by Blocking NKG2A Checkpoint. سامانه مدیریت نشریات علمی, 14(4), 509-520. doi: 10.30476/mejc.2023.97716.1876
Mohammed Taha; Cyril Fauriat. "Improvement of NK Cell Cytotoxicity in Reconstituting NK Cells after Allogeneic Stem Cell Transplantation by Blocking NKG2A Checkpoint". سامانه مدیریت نشریات علمی, 14, 4, 1402, 509-520. doi: 10.30476/mejc.2023.97716.1876
Taha, Mohammed, Fauriat, Cyril. (1402). 'Improvement of NK Cell Cytotoxicity in Reconstituting NK Cells after Allogeneic Stem Cell Transplantation by Blocking NKG2A Checkpoint', سامانه مدیریت نشریات علمی, 14(4), pp. 509-520. doi: 10.30476/mejc.2023.97716.1876
Taha, Mohammed, Fauriat, Cyril. Improvement of NK Cell Cytotoxicity in Reconstituting NK Cells after Allogeneic Stem Cell Transplantation by Blocking NKG2A Checkpoint. سامانه مدیریت نشریات علمی, 1402; 14(4): 509-520. doi: 10.30476/mejc.2023.97716.1876

Improvement of NK Cell Cytotoxicity in Reconstituting NK Cells after Allogeneic Stem Cell Transplantation by Blocking NKG2A Checkpoint

Middle East Journal of Cancer
مقاله 4، دوره 14، شماره 4 - شماره پیاپی 56، دی 2023، صفحه 509-520    اصل مقاله (4.38 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2023.97716.1876
نویسندگان
Mohammed Taha* 1؛ Cyril Fauriat2
1Department of Pharmacology and Medical Sciences, Faculty of Pharmacy, Al-Azhar University of Gaza, Gaza, Palestine
2Cancer Research Center of Marseille (CRCM), CNRS, Faculty of Medicine, Aix Marseille University, Marseille, France
چکیده
Background: One cause of tumor relapse after allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the alteration of the graft-versus-tumor effect of early reconstituting natural killer (NK) cells due to overexpression of the NKG2A inhibitory receptor. This study aims to determine the effect of Monalizumab, an anti- NKG2A receptor, on the effector functions of reconstituting NK cells after allo-HSCT.
Method: In this prospective cohort study, 18 patients with hematological malignancies were divided into three groups: dose 1 group (0.1 mg/kg, n = 5), dose 2 group (0.5 mg/kg, n = 8), and dose 3 group (1 mg/kg, n = 5), and followed up for six months. Blood samples were taken directly before the administration of Monalizumab and at different time points post-treatment. Reconstituting NK cells were phenotypically and functionally assessed by flow cytometry.
Results: Our results showed a more pronounced increase in the expression of activating NK receptors (NKG2D, NKp30, NKp46) on the reconstituting CD56dim NK cells of patients receiving 1 mg/kg of Monalizumab compared with other participants. Additionally, we observed that patients treated with dose 3 of Monalizumab had the highest levels of degranulation compared with other patients and controls. Moreover, we noticed that CD56dim NK cells of dose 2- and dose 3-related patients produced significant levels of perforin, interferon gamma (IFN-γ), and tumor necrosis factor alpha (TNF-α) in response to K562 stimulation post-Monalizumab treatment compared with controls and dose 1-treated patients.
Conclusion: We suggest that using Monalizumab improves the phenotype and cytotoxicity of reconstituting NK cells after allo-HSCT.
کلیدواژه‌ها
Natural killer cells؛ Monalizumab؛ NKG2A؛ Cell cytotoxicity؛ Allogeneic hematopoietic stem cell transplantation
مراجع
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