|تعداد مشاهده مقاله||12,794,513|
|تعداد دریافت فایل اصل مقاله||6,126,698|
Long-Term Effects of Low-Dose Naltrexone on Immunomodulatory Properties of Human Adipose-Derived Mesenchymal Stem Cells
|Iranian Journal of Immunology|
|مقاله 8، دوره 20، شماره 2، شهریور 2023، صفحه 219-231 اصل مقاله (1.47 M)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.22034/iji.2023.95659.2385|
|Romina Kazemi1، 2؛ Mobin Mohammadi2، 3؛ Samira Salimiyan1، 2؛ Sara Aliakbari2؛ Moslem Ahmadi4؛ Mohammad Reza Rahmani* 2، 5|
|1Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.|
|2Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.|
|3Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.|
|4Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.|
|5Zoonosis Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.|
|Background: Low-dose naltrexone (LDN) is involved in the treatment of inflammatory and immune system diseases and can affect immune cells. Mesenchymal stem cells (MSCs) are known for their immunomodulatory effects and the potential for the treatment of certain types of autoimmune diseases.|
Objective: To investigate the long-term effects of LDN on human adipose-derived mesenchymal stem cells (ASCs) to see how their immunomodulatory properties are affected and also how LDN-treated ASCs interact with other immune cells present in peripheral blood mononuclear cells (PBMCs).
Methods: After 14 days of treatment, the ability of LDN-treated ASCs to modulate PBMC proliferation in a two-way mixed lymphocyte reaction (MLR) model was assessed using XTT. The relative expression of IDO, PD-L1, COX-2, HGF genes, and the level of IL-6 and TGF-β cytokines were measured in IFN-γ stimulated and unstimulated ASCs (treated and not treated cells) using real-time PCR and ELISA respectively.
Results: Unstimulated ASCs treated with 10-8 M Naltrexone (10-8 M NTX) showed higher levels of TGF-β, compared with the controls (P<0.05). Stimulated ASCs treated with 10-6 M NTX showed elevated expression of IDO, PD-L1 genes, and IL-6 level (P<0.05).
Conclusion: Our results demonstrated that various LDN concentrations have dissimilar effects on ASCs’ immunomodulatory properties. A higher LDN concentration induced an alteration in the immunomodulatory features of ASCs.
|Adipose-Derived Mesenchymal Stem Cells؛ Immunomodulation؛ Inflammatory Response؛ LDN؛ Naltrexone|
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