Thiol-reducing agents abate cholestasis-induced lung inflammation, oxidative stress, and histopathological alterations | ||
| Trends in Pharmaceutical Sciences and Technologies | ||
| مقاله 6، دوره 9، شماره 1، بهار 2023، صفحه 55-70 اصل مقاله (1.24 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/tips.2023.96536.1165 | ||
| نویسندگان | ||
| Hossein Niknahad1، 2؛ Ali Nadgaran1، 2؛ Sepideh Alidaee1؛ Abdollah Arjmand3؛ Narges Abdoli4؛ Sahra M Mazloomi1؛ Alireza Akhlagh1؛ Ahmad Nikoozadeh1، 2؛ Seyed Mohammad Amin Kashani1، 2؛ Pooria Sayar Mehrabani1، 2؛ Mohammad Rezaei1، 2؛ Mohsen Saeed1، 2؛ Omid Farshad1؛ Amin Reza Akbarizadeh1، 2؛ Asma Najibi1، 2؛ Samira M Sabouri5؛ Negar Azarpira6؛ Mohammad Mehdi Ommati* 1، 5، 7؛ Reza Heidari* 1 | ||
| 1Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Department of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 3Department of Toxicology and Pharmacology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 4Food and Drug Administration, Iran Ministry of Health and Medical Education, Tehran, Iran | ||
| 5Shanxi Key Laboratory of Ecological Animal Sciences and Environmental Veterinary Medicine, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China | ||
| 6Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 7Henan Key Laboratory of Environmental and Animal Product Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, Henan, China | ||
| چکیده | ||
| Cholestasis is not only influences the hepatic function but also damages many other organs. Lung injury is a critical secondary organ damage associated with cholestasis/cirrhosis. Pulmonary histopathological alterations, respiratory distress, and hypoxia are related to cholestasis/cirrhosis-induced lung injury. It has been found that oxidative stress plays a crucial role in this complication. The current study was designed to investigate the effect of N-acetyl cysteine (NAC) and dithiothreitol (DTT) as thiol-reducing and antioxidant agents against cholestasis-induced lung injury. Bile duct ligated (BDL) rats were monitored for the presence of inflammatory cells, TNF-α, and IgG levels in their broncho-alveolar fluid (BALF) at scheduled time intervals (3, 7, 14, and 28 days post-BDL surgery). These markers reached their highest level in the BALF of BDL rats on day 28 after the surgery. Therefore, in another set of experiments, the BDL animals were treated with NAC (100 and 300 mg/kg/day, i.p, for 28 consecutive days) and DTT (10 and 20 mg/kg/day, i.p, for 28 consecutive days). Meanwhile, a significant increase in the levels of TNF-α and IgG was detected in the BALF of BDL rats. The BALF level of neutrophils, monocytes, and lymphocytes was also significantly increased in cholestatic animals. A significant increase in lung tissue biomarkers of oxidative stress was detected in the BDL rats. It was found that NAC and DTT could significantly blunt pulmonary damage induced by cholestasis. The effects of these agents on oxidative stress biomarkers and inflammatory response seem to play a pivotal role in their mechanisms of protective properties. | ||
| کلیدواژهها | ||
| Bile acid؛ Cirrhosis؛ Cholestasis؛ Inflammation؛ Oxidative stress؛ Pulmonary injury | ||
| مراجع | ||
|
| ||
|
آمار تعداد مشاهده مقاله: 22,933 تعداد دریافت فایل اصل مقاله: 33,064 |
||