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Yu, Liquan, Dai, Jian, Fan, Ziyao, Chen, Liang, Liu, Daolong, Wu, Zhijun, Sun, Hunan, Tong, Chunyu, Yu, Yongzhong, Song, Baifen, Ma, Jinzhu, Cui, Yudong. (1401). Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice. سامانه مدیریت نشریات علمی, 20(1), 57-66. doi: 10.22034/iji.2023.82351.1575
Liquan Yu; Jian Dai; Ziyao Fan; Liang Chen; Daolong Liu; Zhijun Wu; Hunan Sun; Chunyu Tong; Yongzhong Yu; Baifen Song; Jinzhu Ma; Yudong Cui. "Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice". سامانه مدیریت نشریات علمی, 20, 1, 1401, 57-66. doi: 10.22034/iji.2023.82351.1575
Yu, Liquan, Dai, Jian, Fan, Ziyao, Chen, Liang, Liu, Daolong, Wu, Zhijun, Sun, Hunan, Tong, Chunyu, Yu, Yongzhong, Song, Baifen, Ma, Jinzhu, Cui, Yudong. (1401). 'Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice', سامانه مدیریت نشریات علمی, 20(1), pp. 57-66. doi: 10.22034/iji.2023.82351.1575
Yu, Liquan, Dai, Jian, Fan, Ziyao, Chen, Liang, Liu, Daolong, Wu, Zhijun, Sun, Hunan, Tong, Chunyu, Yu, Yongzhong, Song, Baifen, Ma, Jinzhu, Cui, Yudong. Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice. سامانه مدیریت نشریات علمی, 1401; 20(1): 57-66. doi: 10.22034/iji.2023.82351.1575

Immunogenicity of the Non-toxic Form of Staphylococcus Aureus α-Hemolysin Using a Chimeric Fusion in Mice

Iranian Journal of Immunology
دوره 20، شماره 1، خرداد 2023، صفحه 57-66    اصل مقاله (1.2 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.82351.1575
نویسندگان
Liquan Yu؛ Jian Dai؛ Ziyao Fan؛ Liang Chen؛ Daolong Liu؛ Zhijun Wu؛ Hunan Sun؛ Chunyu Tong؛ Yongzhong Yu؛ Baifen Song؛ Jinzhu Ma؛ Yudong Cui*
School of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China.
چکیده
Background: Staphylococcus aureus is an opportunistic pathogen responsible for various infections with diverse clinical presentation and severity. The α-hemolysin is a major virulence factor in the pathogenesis of S. aureus infections.
Objective: To produce a chimeric fusion protein for hemolytic detection of the S. aureus isolates and as a component of a multi-antigen vaccine.
Methods: The fused strategy employed a flexible linker to incorporate the possible B cell and T cell determinants into one chimera (HlaD). The humoral and cellular response to the HlaD in mice was assessed to reveal a non-significant difference compared with the full-length α-hemolysin mutant (Hla H35L).
Results: The results of the protective effect, the mimetic lung cell injury, and bacterial clearness demonstrated that the mice vaccinated with the HlaD alleviated the severity of the infection of the S. aureus, and the HlaD could similarly function with Hla H35L.
Conclusion: The chimeric fusion (HlaD) provided a diagnostic antigen for hemolysis of the S. aureus strains and a potential vaccine component.
کلیدواژه‌ها
α-hemolysin؛ Fusion؛ Immunogenicity؛ Staphylococcus aureus
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