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Jiang, Yan, Li, Linqiao, Pan, Qilu, Du, Xiaojing, Han, Qian, Ling, Feixiang, Li, Rou, Mai, Lin, Huang, Jianwei, Chu, Shuyuan, Ma, Libing. (1401). Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin. سامانه مدیریت نشریات علمی, 20(1), 45-56. doi: 10.22034/iji.2023.93312.2212
Yan Jiang; Linqiao Li; Qilu Pan; Xiaojing Du; Qian Han; Feixiang Ling; Rou Li; Lin Mai; Jianwei Huang; Shuyuan Chu; Libing Ma. "Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin". سامانه مدیریت نشریات علمی, 20, 1, 1401, 45-56. doi: 10.22034/iji.2023.93312.2212
Jiang, Yan, Li, Linqiao, Pan, Qilu, Du, Xiaojing, Han, Qian, Ling, Feixiang, Li, Rou, Mai, Lin, Huang, Jianwei, Chu, Shuyuan, Ma, Libing. (1401). 'Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin', سامانه مدیریت نشریات علمی, 20(1), pp. 45-56. doi: 10.22034/iji.2023.93312.2212
Jiang, Yan, Li, Linqiao, Pan, Qilu, Du, Xiaojing, Han, Qian, Ling, Feixiang, Li, Rou, Mai, Lin, Huang, Jianwei, Chu, Shuyuan, Ma, Libing. Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin. سامانه مدیریت نشریات علمی, 1401; 20(1): 45-56. doi: 10.22034/iji.2023.93312.2212

Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin

Iranian Journal of Immunology
دوره 20، شماره 1، خرداد 2023، صفحه 45-56    اصل مقاله (2.71 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.93312.2212
نویسندگان
Yan Jiang؛ Linqiao Li؛ Qilu Pan؛ Xiaojing Du؛ Qian Han؛ Feixiang Ling؛ Rou Li؛ Lin Mai؛ Jianwei Huang؛ Shuyuan Chu؛ Libing Ma*
Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, China.
چکیده
Background: Little is known about MBD2’s epigenetic regulation in the immune pathogenesis of CD4+T cell differentiation.
Objective: This study attempted to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen ovalbumin (OVA).
Methods: Mononuclear cells were separated from the spleen tissues of male C57BL/6 mice. The OVA interfered with the differentiation of splenic mononuclear cells and CD4+T cells. The CD4+T cells were obtained by magnetic beads and identified by CD4 labeled antibody. CD4+T cells were transfected with lentivirus to silence MBD2 gene. A methylation quantification kit was used to detect 5-mC levels.
Results: The purity of CD4+T cells reached 95.99% after magnetic beads sorting. Treatment with 200 μg/mL OVA stimulated the CD4+T cells differentiation to Th17 cells and promoted the secretion of IL-17. After being induced, the Th17 cell ratio increased. 5-Aza inhibited the Th17 cell differentiation and the IL-17 level in a dose-dependent manner. Under the intervention of the Th17 induction and 5-Aza, MBD2 silencing inhibited the differentiation of Th17 cell, and decreased the IL-17 and 5-mC levels in the cell supernatants. MBD2 silencing reduced the scale of the Th17 cell and IL-17 levels in the OVA-treated CD4+T cells.
Conclusion: MBD2 affected IL-17 and 5-mC levels by mediating the Th17 cell differentiation in splenic CD4+T cells that were interfered with 5-Aza. OVA induced Th17 differentiation and increased IL-17 levels, inhibited by MBD2 silencing.
کلیدواژه‌ها
CD4+T Cell؛ IL-17؛ MBD2؛ Ovalbumin؛ Th17 Cell
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