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Lu, Jian-Hua, Rui, Xiao-Xiao, Wang, Ting-Ting, Wang, Xiao-Qin, Peng, Yu-Ping, Qiu, Yi-Hua. (1401). Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance. سامانه مدیریت نشریات علمی, 20(1), 16-25. doi: 10.22034/iji.2023.94125.2286
Jian-Hua Lu; Xiao-Xiao Rui; Ting-Ting Wang; Xiao-Qin Wang; Yu-Ping Peng; Yi-Hua Qiu. "Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance". سامانه مدیریت نشریات علمی, 20, 1, 1401, 16-25. doi: 10.22034/iji.2023.94125.2286
Lu, Jian-Hua, Rui, Xiao-Xiao, Wang, Ting-Ting, Wang, Xiao-Qin, Peng, Yu-Ping, Qiu, Yi-Hua. (1401). 'Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance', سامانه مدیریت نشریات علمی, 20(1), pp. 16-25. doi: 10.22034/iji.2023.94125.2286
Lu, Jian-Hua, Rui, Xiao-Xiao, Wang, Ting-Ting, Wang, Xiao-Qin, Peng, Yu-Ping, Qiu, Yi-Hua. Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance. سامانه مدیریت نشریات علمی, 1401; 20(1): 16-25. doi: 10.22034/iji.2023.94125.2286

Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance

Iranian Journal of Immunology
دوره 20، شماره 1، خرداد 2023، صفحه 16-25    اصل مقاله (1.71 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2023.94125.2286
نویسندگان
Jian-Hua Lu؛ Xiao-Xiao Rui؛ Ting-Ting Wang؛ Xiao-Qin Wang؛ Yu-Ping Peng؛ Yi-Hua Qiu*
Department of Physiology, School of Medicine, and Co-innovation Center of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu Province 226001, China.
چکیده
Background: Recent research in our laboratory shows that CD4+ T cells express the β2 adrenergic receptor (β2-AR), and the sympathetic neurotransmitter norepinephrine regulates the function of T cells via β2-AR signaling. However, the immunoregulatory effect of β2-AR and its related mechanisms on rheumatoid arthritis is unknown.
Objective: To explore the effects of β2-AR in collagen-induced arthritis (CIA) on the imbalance of T helper (Th) 17/ regulatory T (Treg) cells.
Methods: In DBA1/J mice, collagen type II was injected intradermally at the tail base to prepare the CIA model. The specific β2-AR agonist, terbutaline (TBL), was administered intraperitoneally beginning on day 31 and continuing until day 47 after primary vaccination, twice a day. Magnetic beads were used to sort CD3+ T cells subsets from spleen tissues.
Results: In vivo, β2-AR agonist TBL alleviated arthritis symptoms in the CIA mice including histopathology of the ankle joints, four limbs’ arthritis score, the thickness of ankle joints, and rear paws. After TBL treatment, in the ankle joints, the levels of proinflammatory factors (IL-17/22) notably decreased and the levels of immunosuppressive factors (IL-10/TGF-β) significantly increased. In vitro, ROR-γt protein expression, Th17 cell number, mRNA expression and the releasing of IL-17/22 from CD3+ T cells reduced following TBL administration. Moreover, TBL enhanced the anti-inflammatory responses of Treg cells.
Conclusion: These results suggest that β2-AR activation exerts anti-inflammatory effects through the amelioration of Th17/Treg imbalance in the CIA disease.
کلیدواژه‌ها
β2-adrenergic receptor؛ Inflammation؛ Terbutaline؛ Th17 cells؛ Treg cells؛ Type II collagen-induced Arthritis
مراجع
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