LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,113
تعداد مقالات10,208
تعداد مشاهده مقاله40,780,184
تعداد دریافت فایل اصل مقاله8,803,957
Afolayan, Michael, Asekun, Olayinka, Familoni, Oluwole. (1401). In Vitro Antimalarial, Antimicrobial and Trypanocidal Potentials of the Leaf Extract and Fractions of Bridelia ferruginea (Benth.). سامانه مدیریت نشریات علمی, 9(1), 27-34. doi: 10.30476/tips.2023.97796.1179
Michael O Afolayan; Olayinka Asekun; Oluwole Familoni. "In Vitro Antimalarial, Antimicrobial and Trypanocidal Potentials of the Leaf Extract and Fractions of Bridelia ferruginea (Benth.)". سامانه مدیریت نشریات علمی, 9, 1, 1401, 27-34. doi: 10.30476/tips.2023.97796.1179
Afolayan, Michael, Asekun, Olayinka, Familoni, Oluwole. (1401). 'In Vitro Antimalarial, Antimicrobial and Trypanocidal Potentials of the Leaf Extract and Fractions of Bridelia ferruginea (Benth.)', سامانه مدیریت نشریات علمی, 9(1), pp. 27-34. doi: 10.30476/tips.2023.97796.1179
Afolayan, Michael, Asekun, Olayinka, Familoni, Oluwole. In Vitro Antimalarial, Antimicrobial and Trypanocidal Potentials of the Leaf Extract and Fractions of Bridelia ferruginea (Benth.). سامانه مدیریت نشریات علمی, 1401; 9(1): 27-34. doi: 10.30476/tips.2023.97796.1179

In Vitro Antimalarial, Antimicrobial and Trypanocidal Potentials of the Leaf Extract and Fractions of Bridelia ferruginea (Benth.)

Trends in Pharmaceutical Sciences
مقاله 3، دوره 9، شماره 1، خرداد 2023، صفحه 27-34    اصل مقاله (458.59 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/tips.2023.97796.1179
نویسندگان
Michael O Afolayan* 1، 2؛ Olayinka Asekun2؛ Oluwole Familoni2
1Chemistry Advanced Research Centre, Sheda Science and Technology Complex (SHESTCO), P.M.B. 186, Garki – Abuja, Nigeria
2Department of Chemistry, University of Lagos, Lagos, Nigeria.
چکیده
Bridelia ferruginea Benth. (Euphorbiaceae) is a plant used for various medicinal purposes including the treatment of protozoan infections in many African countries. The present study was aimed to evaluate the in vitro antimalarial, antimicrobial and antitrypanasomal activity of the methanol crude extract and fractions of the medicinal plant, Bridelia ferruginea. Crude extract from dried leaves of Bridelia ferruginea was prepared by cold maceration and fractionated using a reverse phase column under reduced pressure with water – methanol mixtures. The extracts and fractions were tested to determine their biological activities. In vitro antimalarial activity was determined by the plasmodial lactate dehydrogenase culture sensitivity assay while antitrypanosomal activity was tested in vitro against a culture of Trypanosoma brucei promastigotes. The crude extract of B. ferruginea exhibited significant antitrypanasomal activity with an IC50 of 8.48 µg/mL which was further enhanced in some of the fractions like the MeOH fraction (BFD) with an IC50 of 1.04 µg/Ml which indicates very good activity. Sub – fraction BFF (obtained from 1:3 H2O:MeOH fraction – BFC) exhibited the best antimalarial activity among all the fractions and sub – fractions with an IC50 value of 19.73 µg/mL against P. falciparum D6 and 20.49 µg/mL against P. falciparum W2. There was no observable antibacterial or antifungal activity for all crude extracts, fractions and sub – fractions. The results from this study have validated the leaves of Bridelia ferruginea as potential sources of antimalarial and antitrypanosomal agents which can serve as a lead in drug development.
کلیدواژه‌ها
Antimalarial؛ Antimicrobial؛ Antitrypanosomal. Bridelia ferruginea؛ in vitro
مراجع
1.    Cragg GM, Newman DJ. Natural products: a continuing source of novel drug leads. Biochim Biophys Acta. 2013 Jun;1830(6):3670-95. doi: 10.1016/j.bbagen.2013.02.008. Epub 2013 Feb 18. PMID: 23428572; PMCID: PMC3672862.
2.    Gurib-Fakim A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med. 2006 Feb;27(1):1-93. doi: 10.1016/j.mam.2005.07.008. Epub 2005 Aug 18. PMID: 16105678.
3.    Okigbo RN, Eme UE, Ogbogu S. Biodiversity and conservation of medicinal and aromatic plants in Africa. Biotechnol Mol Biol Rev. 2008;3(6):127-134
4.    Afolayan MO, Omosimua RO, Fadeyi AE, Aguzue OC, Asekun OT, Familoni OB. Phytochemical, Antioxidant, and Flavonoid Investigation of Methanolic Leaf Extract of Piliostigma thonningii (Schum.). Natural Product Experiments in Drug Discovery. 2023;1-26). Humana, New York, NY.
5.    Rates SM. Plants as source of drugs. Toxicon. 2001 May;39(5):603-13. doi: 10.1016/s0041-0101(00)00154-9. PMID: 11072038.
6.    Ekanem JT, Kolawole OM, Abbah OC. Trypanocidal potential of methanolic extract of Bridelia ferruginea benth bark in Rattus novergicus. Afr J Biochem Res. 2008;2 (2):045-050.
7.    Afolayan M, Srivedavyasasri R, Asekun OT, Familoni OB, Ross SA. Chemical and biological studies on Bridelia ferruginea grown in Nigeria. Nat Prod Res. 2019 Jan;33(2):287-291. doi: 10.1080/14786419.2018.1440225. Epub 2018 Feb 19. PMID: 29457749; PMCID: PMC6098982.
8.    Cimanga K, De Bruyne T, Apers S, Pieters L, Totté J, Kambu K, Tona L, Bakana P, van Ufford LQ, Beukelman C, Labadie R, Vlietinck AJ. Complement-Inhibiting Constituents of Bridelia ferruginea Stem Bark. Planta Med. 1999 Apr;65(3):213-7. doi: 10.1055/s-1999-14059. PMID: 17260306.
9.    Orafidiya LO, Lamikanra A, Adediji JA. Coagulation of milk as an index of astringency of the bark extract of Bridelia ferruginea benth and lime juice for the formulation of a traditional gargle ‘Ogun Efu’. Phytother Res. 1990;4(5):189-194.
10.    Kolawole OM, Oguntoye SO, Agbede O,  Olayemi AB. Studies on the efficacy of Bridelia ferruginea benth bark extract for domestic wastewater treatment. Bull Chem Soc Ethiop. 2007;21(2):205-211.
11.    Bruyne TD, Cimanga K, Pieters L, Claeys, M, Dommisse R, Vlietinck A. Gallocatechin–(4′→ 0→ 7)-epigallocatechin, a new biflavonoid isolated from Bridelia Ferruginea. Nat Prod Lett. 1997;11(1):47-52.
12.    Pettit GR, Searcy JD, Tan R, Cragg GM, Melody N, Knight JC, Chapuis JC. Antineoplastic Agents. 585. Isolation of Bridelia ferruginea Anticancer Podophyllotoxins and Synthesis of 4-Aza-podophyllotoxin Structural Modifications. J Nat Prod. 2016 Mar 25;79(3):507-18. doi: 10.1021/acs.jnatprod.5b00873. Epub 2016 Mar 3. PMID: 26938998.
13.    Ma G, Khan SI, Jacob MR, Tekwani BL, Li Z, Pasco DS, Walker LA, Khan IA. Antimicrobial and antileishmanial activities of hypocrellins A and B. Antimicrob Agents Chemother. 2004 Nov;48(11):4450-2. doi: 10.1128/AAC.48.11.4450-4452.2004. PMID: 15504880; PMCID: PMC525422.
14.    Bharate SB, Khan SI, Yunus NA, Chauthe SK, Jacob MR, Tekwani BL, Khan IA, Singh IP. Antiprotozoal and antimicrobial activities of O-alkylated and formylated acylphloroglucinols. Bioorg Med Chem. 2007 Jan 1;15(1):87-96. doi: 10.1016/j.bmc.2006.10.006. Epub 2006 Oct 10. PMID: 17070063.
15.    Makler MT, Hinrichs DJ. Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. Am J Trop Med Hyg. 1993 Feb;48(2):205-10. doi: 10.4269/ajtmh.1993.48.205. PMID: 8447524.
16.    NCCLS. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. M7-A7.20. Clinical Laboratory Standard Institute. 2002;26 (2):1-58.
17.    NCCLS. Reference method for broth dilution antifungal susceptibility testing of yeasts, approved standard. Clinical Laboratory Standard Institute document M27-A2. 2002;22 (15):1-51.
18.    NCCLS. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi, approved standard. M38-A2. Clinical Laboratory Standard Institute. 2002;28 (16):1-35. 
19.    NCCLS. Susceptibility testing of mycobacterium and other aerobic actinomycetes tentative standard-Second Edition, M24-T2. Clinical Laboratory Standard Institute.2002;20 (26):1-81.
20.    Radwan MM, Manly SP, Ross SA. Two new sulfated sterols from the marine sponge Lendenfeldia dendyi. Nat Prod Commun. 2007;2 (9):901-4
21.    Mbah CC, Akuodor GC, Anyalewechi NA, Iwuanyanwu TC, Osunkwo UA. In vivo antiplasmodial activities of aqueous extract of Bridelia ferruginea stem bark against Plasmodium berghei berghei in mice. Pharm Biol. 2012 Feb;50(2):188-94. doi: 10.3109/13880209.2011.592537. PMID: 22235887.
22.    Adebayo EA, Ishola OR. Phytochemical and antimicrobial screening of the crude extracts from the root, stem bark and leaves of Bridelia ferruginea. Afr J Biotech. 2009;8 (4):650-53

آمار
تعداد مشاهده مقاله: 16,796
تعداد دریافت فایل اصل مقاله: 412


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب