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Moezi, Leila, Pirsalami, Fatema, Dastgheib, Mona, Oftadehgan, Somayeh, Purkhosrow, Azar, Sattarinezhad, Elahe. (1402). Acute and Sub-chronic Anticonvulsant Effects of Edaravone on Seizure Induced by Pentylenetetrazole or Electroshock in Mice, Nitric Oxide Involvement. سامانه مدیریت نشریات علمی, 48(3), 329-340. doi: 10.30476/ijms.2022.94177.2544
Leila Moezi; Fatema Pirsalami; Mona Dastgheib; Somayeh Oftadehgan; Azar Purkhosrow; Elahe Sattarinezhad. "Acute and Sub-chronic Anticonvulsant Effects of Edaravone on Seizure Induced by Pentylenetetrazole or Electroshock in Mice, Nitric Oxide Involvement". سامانه مدیریت نشریات علمی, 48, 3, 1402, 329-340. doi: 10.30476/ijms.2022.94177.2544
Moezi, Leila, Pirsalami, Fatema, Dastgheib, Mona, Oftadehgan, Somayeh, Purkhosrow, Azar, Sattarinezhad, Elahe. (1402). 'Acute and Sub-chronic Anticonvulsant Effects of Edaravone on Seizure Induced by Pentylenetetrazole or Electroshock in Mice, Nitric Oxide Involvement', سامانه مدیریت نشریات علمی, 48(3), pp. 329-340. doi: 10.30476/ijms.2022.94177.2544
Moezi, Leila, Pirsalami, Fatema, Dastgheib, Mona, Oftadehgan, Somayeh, Purkhosrow, Azar, Sattarinezhad, Elahe. Acute and Sub-chronic Anticonvulsant Effects of Edaravone on Seizure Induced by Pentylenetetrazole or Electroshock in Mice, Nitric Oxide Involvement. سامانه مدیریت نشریات علمی, 1402; 48(3): 329-340. doi: 10.30476/ijms.2022.94177.2544

Acute and Sub-chronic Anticonvulsant Effects of Edaravone on Seizure Induced by Pentylenetetrazole or Electroshock in Mice, Nitric Oxide Involvement

Iranian Journal of Medical Sciences
مقاله 11، دوره 48، شماره 3، مرداد 2023، صفحه 329-340    اصل مقاله (1.57 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2022.94177.2544
نویسندگان
Leila Moezi؛ Fatema Pirsalami؛ Mona Dastgheib؛ Somayeh Oftadehgan؛ Azar Purkhosrow؛ Elahe Sattarinezhad*
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Edaravone is an anti-stroke medication that may have nitric oxide (NO) modulating properties. This study evaluated the role of NO in the acute and sub-chronic anticonvulsant effects of edaravone in murine models of seizures induced by intraperitoneal (IP) or intravenous (IV) injections of pentylenetetrazole (PTZ) or electroshock (maximal electroshock seizure [MES]).
Methods: 132 male albino mice were randomly divided into 22 groups (n=6) and given IP injections of vehicle or edaravone either acutely or for eight days (sub-chronically). The seizure was induced by electroshock or PTZ (IP or IV). The following edaravone doses were used: 7.5, 10, 12.5 (acute); 5, 7.5, 10 (sub-chronic) in IP PTZ model; 5, 7.5, 10 in IV PTZ model; and 5, 10 mg/Kg in the MES. To evaluate NO involvement, 216 mice were randomly divided into 36 groups (n=6) and pretreated with vehicle, edaravone, a non-specific nitric oxide synthase (NOS) inhibitor: N(ω)-nitro-L-arginine methyl ester (L-NAME) (5 mg/Kg), a specific nNOS inhibitor: 7-nitroindazole (7-NI) (60 mg/Kg), or a combination of edaravone plus L-NAME or 7-NI, either acutely or for eight days before seizure induction. Doses of edaravone were as follows: in IP PTZ model: 12.5 (acute) and 10 (sub-chronic); in IV PTZ model: 10; and in the MES: 5 mg/Kg. Data were analyzed using the one-way analysis of variance (ANOVA) followed by Tukey’s test (SPSS 18). P≤0.05 was considered statistically significant.
Results: In the IP PTZ model, edaravone increased time latencies to seizures (P<0.001), prevented tonic seizures, and death. Edaravone increased the seizure threshold (P<0.001) in the IV PTZ model and shortened the duration of tonic hind-limb extension (THE) in the MES model (P<0.001). In comparison to mice treated with edaravone alone, adding L-NAME or 7-NI reduced seizure time latencies (P<0.001), reduced seizure threshold (P<0.001), and increased THE duration (P<0.001).
Conclusion: Edaravone (acute or sub-chronic) could prevent seizures by modulating NO signaling pathways.
کلیدواژه‌ها
Edaravone؛ Epilepsy؛ Pentylenetetrazole؛ Electroshock؛ Nitric oxide
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مراجع
  1. Lee SK. Old versus New: Why Do We Need New Antiepileptic Drugs? J Epilepsy Res. 2014;4:39-44. doi: 10.14581/jer.14010. PubMed PMID: 25625087; PubMed Central PMCID: PMCPMC4295052.
  2. Fattorusso A, Matricardi S, Mencaroni E, Dell’Isola GB, Di Cara G, Striano P, et al. The Pharmacoresistant Epilepsy: An Overview on Existant and New Emerging Therapies. Front Neurol. 2021;12:674483. doi: 10.3389/fneur.2021.674483. PubMed PMID: 34239494; PubMed Central PMCID: PMCPMC8258148.
  3. Perucca E. The pharmacological treatment of epilepsy: recent advances and future perspectives. Acta Epileptologica. 2021;3:1-11. doi: 10.1186/s42494-021-00055-z.
  4. Loscher W, Potschka H, Sisodiya SM, Vezzani A. Drug Resistance in Epilepsy: Clinical Impact, Potential Mechanisms, and New Innovative Treatment Options. Pharmacol Rev. 2020;72:606-38. doi: 10.1124/pr.120.019539. PubMed PMID: 32540959; PubMed Central PMCID: PMCPMC7300324.
  5. Watanabe K, Tanaka M, Yuki S, Hirai M, Yamamoto Y. How is edaravone effective against acute ischemic stroke and amyotrophic lateral sclerosis? J Clin Biochem Nutr. 2018;62:20-38. doi: 10.3164/jcbn.17-62. PubMed PMID: 29371752; PubMed Central PMCID: PMCPMC5773834.
  6. Kawasaki H, Ito Y, Kitabayashi C, Tanaka A, Nishioka R, Yamazato M, et al. Effects of Edaravone on Nitric Oxide, Hydroxyl Radicals and Neuronal Nitric Oxide Synthase During Cerebral Ischemia and Reperfusion in Mice. J Stroke Cerebrovasc Dis. 2020;29:104531. doi: 10.1016/j.jstrokecerebrovasdis.2019.104531. PubMed PMID: 31882337.
  7. Yoshida H, Kwon AH, Kaibori M, Tsuji K, Habara K, Yamada M, et al. Edaravone prevents iNOS expression by inhibiting its promoter transactivation and mRNA stability in cytokine-stimulated hepatocytes. Nitric Oxide. 2008;18:105-12. doi: 10.1016/j.niox.2007.11.003. PubMed PMID: 18078833.
  8. Dzoljic E, Grbatinic I, Kostic V. Why is nitric oxide important for our brain? Funct Neurol. 2015;30:159-63. doi: 10.11138/fneur/2015.30.3.159. PubMed PMID: 26910176; PubMed Central PMCID: PMCPMC4610750.
  9. Banach M, Piskorska B, Czuczwar SJ, Borowicz KK. Nitric oxide, epileptic seizures, and action of antiepileptic drugs. CNS Neurol Disord Drug Targets. 2011;10:808-19. doi: 10.2174/187152711798072347. PubMed PMID: 21999730.
  10. Przegaliñski E, Baran L, Siwanowicz J. The role of nitric oxide in chemically-and electrically-induced seizures in mice. Neuroscience letters. 1996;217:145-8. doi: 10.1016/0304-3940(96)13085-8.
  11. Zhu X, Dong J, Han B, Huang R, Zhang A, Xia Z, et al. Neuronal Nitric Oxide Synthase Contributes to PTZ Kindling Epilepsy-Induced Hippocampal Endoplasmic Reticulum Stress and Oxidative Damage. Front Cell Neurosci. 2017;11:377. doi: 10.3389/fncel.2017.00377. PubMed PMID: 29234274; PubMed Central PMCID: PMCPMC5712337.
  12. Del-Bel EA, Oliveira PR, Oliveira JA, Mishra PK, Jobe PC, Garcia-Cairasco N. Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models. Braz J Med Biol Res. 1997;30:971-9. doi: 10.1590/s0100-879x1997000800010. PubMed PMID: 9361727.
  13. Rasooli R, Pirsalami F, Moezi L. Posible involvement of nitric oxide in anticonvulsant effects of citicoline on pentylenetetrazole and electroshock induced seizures in mice. Heliyon. 2020;6:e03932. doi: 10.1016/j.heliyon.2020.e03932. PubMed PMID: 32462085; PubMed Central PMCID: PMCPMC7240119.
  14. Kovacs R, Rabanus A, Otahal J, Patzak A, Kardos J, Albus K, et al. Endogenous nitric oxide is a key promoting factor for initiation of seizure-like events in hippocampal and entorhinal cortex slices. J Neurosci. 2009;29:8565-77. doi: 10.1523/JNEUROSCI.5698-08.2009. PubMed PMID: 19571147; PubMed Central PMCID: PMCPMC6665664.
  15. Sharma S, Puttachary S, Thippeswamy T. Glial source of nitric oxide in epileptogenesis: A target for disease modification in epilepsy. J Neurosci Res. 2019;97:1363-77. doi: 10.1002/jnr.24205. PubMed PMID: 29230865; PubMed Central PMCID: PMCPMC6035106.
  16. Hopper R, Lancaster B, Garthwaite J. On the regulation of NMDA receptors by nitric oxide. Eur J Neurosci. 2004;19:1675-82. doi: 10.1111/j.1460-9568.2004.03306.x. PubMed PMID: 15078541.
  17. Paul V, Jayakumar AR. A role of nitric oxide as an inhibitor of gamma-aminobutyric acid transaminase in rat brain. Brain Res Bull. 2000;51:43-6. doi: 10.1016/s0361-9230(99)00206-3. PubMed PMID: 10654579.
  18. Kourosh-Arami M, Hosseini N, Mohsenzadegan M, Komaki A, Joghataei MT. Neurophysiologic implications of neuronal nitric oxide synthase. Rev Neurosci. 2020;31:617-36. doi: 10.1515/revneuro-2019-0111. PubMed PMID: 32739909.
  19. Picon-Pages P, Garcia-Buendia J, Munoz FJ. Functions and dysfunctions of nitric oxide in brain. Biochim Biophys Acta Mol Basis Dis. 2019;1865:1949-67. doi: 10.1016/j.bbadis.2018.11.007. PubMed PMID: 30500433.
  20. Cinelli MA, Do HT, Miley GP, Silverman RB. Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 2020;40:158-89. doi: 10.1002/med.21599. PubMed PMID: 31192483; PubMed Central PMCID: PMCPMC6908786.
  21. Lin TK, Chen SD, Lin KJ, Chuang YC. Seizure-Induced Oxidative Stress in Status Epilepticus: Is Antioxidant Beneficial? Antioxidants (Basel). 2020;9. doi: 10.3390/antiox9111029. PubMed PMID: 33105652; PubMed Central PMCID: PMCPMC7690410.
  22. Tieu K, Ischiropoulos H, Przedborski S. Nitric oxide and reactive oxygen species in Parkinson’s disease. IUBMB Life. 2003;55:329-35. doi: 10.1080/1521654032000114320. PubMed PMID: 12938735.
  23. Toda N, Ayajiki K, Okamura T. Cerebral blood flow regulation by nitric oxide in neurological disorders. Can J Physiol Pharmacol. 2009;87:581-94. doi: 10.1139/y09-048. PubMed PMID: 19767882.
  24. Schuman EM, Madison DV. Nitric oxide and synaptic function. Annu Rev Neurosci. 1994;17:153-83. doi: 10.1146/annurev.ne.17.030194.001101. PubMed PMID: 7516125.
  25. Toda N, Okamura T. The pharmacology of nitric oxide in the peripheral nervous system of blood vessels. Pharmacol Rev. 2003;55:271-324. doi: 10.1124/pr.55.2.3. PubMed PMID: 12773630.
  26. Reis PA, de Albuquerque CFG, Gutierrez T, Silva AR, de Castro Faria Neto HC. Role of nitric oxide synthase in the function of the central nervous system under normal and infectious conditions. Nitric Oxide Synthase–Simple Enzyme-Complex Roles London: InTech. 2017:55-70. doi: 10.5772/67816.
  27. Ahmadi-Noorbakhsh S, Mirabzadeh Ardakani E, Sadighi J, Aldavood SJ, Farajli Abbasi M, Farzad-Mohajeri S, et al. Guideline for the Care and Use of Laboratory Animals in Iran. Lab Anim (NY). 2021;50:303-5. doi: 10.1038/s41684-021-00871-3. PubMed PMID: 34621075.
  28. Koutroumanidou E, Kimbaris A, Kortsaris A, Bezirtzoglou E, Polissiou M, Charalabopoulos K, et al. Increased seizure latency and decreased severity of pentylenetetrazol-induced seizures in mice after essential oil administration. Epilepsy Res Treat. 2013;2013:532657. doi: 10.1155/2013/532657. PubMed PMID: 23819045; PubMed Central PMCID: PMCPMC3684096.
  29. Pourgholami MH, Kamalinejad M, Javadi M, Majzoob S, Sayyah M. Evaluation of the anticonvulsant activity of the essential oil of Eugenia caryophyllata in male mice. J Ethnopharmacol. 1999;64:167-71. doi: 10.1016/s0378-8741(98)00121-4. PubMed PMID: 10197752.
  30. Mandhane SN, Aavula K, Rajamannar T. Timed pentylenetetrazol infusion test: a comparative analysis with s.c.PTZ and MES models of anticonvulsant screening in mice. Seizure. 2007;16:636-44. doi: 10.1016/j.seizure.2007.05.005. PubMed PMID: 17570689.
  31. Sattarinezhad E, Shafaroodi H, Sheikhnouri K, Mousavi Z, Moezi L. The effects of coenzyme Q10 on seizures in mice: the involvement of nitric oxide. Epilepsy Behav. 2014;37:36-42. doi: 10.1016/j.yebeh.2014.05.024. PubMed PMID: 24972157.
  32. Castel-Branco MM, Alves GL, Figueiredo IV, Falcao AC, Caramona MM. The maximal electroshock seizure (MES) model in the preclinical assessment of potential new antiepileptic drugs. Methods Find Exp Clin Pharmacol. 2009;31:101-6. doi: 10.1358/mf.2009.31.2.1338414. PubMed PMID: 19455265.
  33. Kandratavicius L, Balista PA, Lopes-Aguiar C, Ruggiero RN, Umeoka EH, Garcia-Cairasco N, et al. Animal models of epilepsy: use and limitations. Neuropsychiatr Dis Treat. 2014;10:1693-705. doi: 10.2147/NDT.S50371. PubMed PMID: 25228809; PubMed Central PMCID: PMCPMC4164293.
  34. Loscher W, Honack D, Fassbender CP, Nolting B. The role of technical, biological and pharmacological factors in the laboratory evaluation of anticonvulsant drugs. III. Pentylenetetrazole seizure models. Epilepsy Res. 1991;8:171-89. doi: 10.1016/0920-1211(91)90062-k. PubMed PMID: 1907909.
  35. Keogh BP, Cordes D, Stanberry L, Figler BD, Robbins CA, Tempel BL, et al. BOLD-fMRI of PTZ-induced seizures in rats. Epilepsy Res. 2005;66:75-90. doi: 10.1016/j.eplepsyres.2005.07.008. PubMed PMID: 16144756.
  36. Ramanjaneyulu R, Ticku MK. Interactions of pentamethylenetetrazole and tetrazole analogues with the picrotoxinin site of the benzodiazepine-GABA receptor-ionophore complex. Eur J Pharmacol. 1984;98:337-45. doi: 10.1016/0014-2999(84)90282-6. PubMed PMID: 6327331.
  37. Giardina WJ, Gasior M. Acute seizure tests in epilepsy research: electroshock- and chemical-induced convulsions in the mouse. Curr Protoc Pharmacol. 2009;5:22. doi: 10.1002/0471141755.ph0522s45. PubMed PMID: 22294398.
  38. Giordano C, Vinet J, Curia G, Biagini G. Repeated 6-Hz Corneal Stimulation Progressively Increases FosB/DeltaFosB Levels in the Lateral Amygdala and Induces Seizure Generalization to the Hippocampus. PLoS One. 2015;10:e0141221. doi: 10.1371/journal.pone.0141221. PubMed PMID: 26555229; PubMed Central PMCID: PMCPMC4640822.
  39. Kamida T, Fujiki M, Ooba H, Anan M, Abe T, Kobayashi H. Neuroprotective effects of edaravone, a free radical scavenger, on the rat hippocampus after pilocarpine-induced status epilepticus. Seizure. 2009;18:71-5. doi: 10.1016/j.seizure.2008.06.012. PubMed PMID: 18672383.
  40. Kurt S, Aygun H. Anticonvulsive effects of edaravone on penicillin-induced focal onset seizure model in the conscious rats. Fundam Clin Pharmacol. 2021;35:861-9. doi: 10.1111/fcp.12651. PubMed PMID: 33484001.
  41. Kamida T, Abe E, Abe T, Ooba H, Fujiki M, Kobayashi H. Edaravone, a free radical scavenger, retards the development of amygdala kindling in rats. Neurosci Lett. 2009;461:298-301. doi: 10.1016/j.neulet.2009.06.039. PubMed PMID: 19545611.
  42. Liu LM, Wang N, Lu Y, Wang WP. Edaravone acts as a potential therapeutic drug against pentylenetetrazole-induced epilepsy in male albino rats by downregulating cyclooxygenase-II. Brain Behav. 2019;9:e01156. doi: 10.1002/brb3.1156. PubMed PMID: 30506635; PubMed Central PMCID: PMCPMC6346642.
  43. Zhang XH, Matsuda N, Jesmin S, Sakuraya F, Gando S, Kemmotsu O, et al. Normalization by edaravone, a free radical scavenger, of irradiation-reduced endothelial nitric oxide synthase expression. Eur J Pharmacol. 2003;476:131-7. doi: 10.1016/s0014-2999(03)02151-4. PubMed PMID: 12969758.
  44. Takahashi G, Sakurai M, Abe K, Itoyama Y, Tabayashi K. MCI-186 prevents spinal cord damage and affects enzyme levels of nitric oxide synthase and Cu/Zn superoxide dismutase after transient ischemia in rabbits. J Thorac Cardiovasc Surg. 2003;126:1461-6. doi: 10.1016/s0022-5223(03)00693-7. PubMed PMID: 14666020.
  45. Yoshida H, Yanai H, Namiki Y, Fukatsu-Sasaki K, Furutani N, Tada N. Neuroprotective effects of edaravone: a novel free radical scavenger in cerebrovascular injury. CNS Drug Rev. 2006;12:9-20. doi: 10.1111/j.1527-3458.2006.00009.x. PubMed PMID: 16834755; PubMed Central PMCID: PMCPMC6741743.
  46. Semenza GL. New insights into nNOS regulation of vascular homeostasis. J Clin Invest. 2005;115:2976-8. doi: 10.1172/JCI26792. PubMed PMID: 16276412; PubMed Central PMCID: PMCPMC1265879.
  47. BAIG MS, Bakhshi F, Ye RD, Gao X, Malik AB, Minshall RD, et al. Role of nNOS in the progression of systemic inflammatory response induced by lipopolysaccharide. Wiley Online Library. 2012. doi: 10.1096/fasebj.26.1_supplement.lb546.
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