LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,416,084
تعداد دریافت فایل اصل مقاله11,291,756
Asgari, Qasem, Zandavi, Seyedeh-Zahra, Moemenbellah-Fard, Mohammad Djaefar, Hosseinpour, Amin, Kalantari, Mohsen, Soltani, Aboozar. (1401). In Vivo and in Vitro Investigations of the Effects of Iranian Honeybee (Hymenoptera: Apis mellifera) Venom and Propolis on Leishmania Major. سامانه مدیریت نشریات علمی, 11(1), 34-41. doi: 10.30476/jhsss.2021.91889.1248
Qasem Asgari; Seyedeh-Zahra Zandavi; Mohammad Djaefar Moemenbellah-Fard; Amin Hosseinpour; Mohsen Kalantari; Aboozar Soltani. "In Vivo and in Vitro Investigations of the Effects of Iranian Honeybee (Hymenoptera: Apis mellifera) Venom and Propolis on Leishmania Major". سامانه مدیریت نشریات علمی, 11, 1, 1401, 34-41. doi: 10.30476/jhsss.2021.91889.1248
Asgari, Qasem, Zandavi, Seyedeh-Zahra, Moemenbellah-Fard, Mohammad Djaefar, Hosseinpour, Amin, Kalantari, Mohsen, Soltani, Aboozar. (1401). 'In Vivo and in Vitro Investigations of the Effects of Iranian Honeybee (Hymenoptera: Apis mellifera) Venom and Propolis on Leishmania Major', سامانه مدیریت نشریات علمی, 11(1), pp. 34-41. doi: 10.30476/jhsss.2021.91889.1248
Asgari, Qasem, Zandavi, Seyedeh-Zahra, Moemenbellah-Fard, Mohammad Djaefar, Hosseinpour, Amin, Kalantari, Mohsen, Soltani, Aboozar. In Vivo and in Vitro Investigations of the Effects of Iranian Honeybee (Hymenoptera: Apis mellifera) Venom and Propolis on Leishmania Major. سامانه مدیریت نشریات علمی, 1401; 11(1): 34-41. doi: 10.30476/jhsss.2021.91889.1248

In Vivo and in Vitro Investigations of the Effects of Iranian Honeybee (Hymenoptera: Apis mellifera) Venom and Propolis on Leishmania Major

Journal of Health Sciences & Surveillance System
دوره 11، شماره 1، فروردین 2023، صفحه 34-41    اصل مقاله (1.48 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/jhsss.2021.91889.1248
نویسندگان
Qasem Asgari1؛ Seyedeh-Zahra Zandavi2؛ Mohammad Djaefar Moemenbellah-Fard3؛ Amin Hosseinpour2؛ Mohsen Kalantari3؛ Aboozar Soltani* 3
1Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
3Research Center for Health Sciences, Institute of Health, Department of Medical Entomology and Vector Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Leishmaniasis, as a neglected health issue, is spreading in most parts of the world. It is one of the most important vector-borne diseases in Iran. Bee venom has shown a wide range of medicinal properties. The present study aimed to survey the effect of venom and propolis of Apis mellifera on Leishmania major in different environments, including in vivo and in vitro.
Methods: In this experimental study, bee venom was extracted using the modified Benton method, and propolis was prepared by the soxhletation method. The promastigotes of L. major were exposed to the different doses of the venom (0.03125-1 μg/ml) and propolis (2.5-80 μg/ml) and then evaluated by MTT assay and Flowcytometry after 24 hours. In vivo phases, 107 promastigotes of the L. major in stationary phase were intradermally inoculated into 48 mice based on the study design. After appearance of the wounds, the mice were topically treated with the lotion containing different doses (5 and 10 μg/ml) of the venom and propolis. The size of the ulcers was measured for four weeks.
Results: The results showed that propolis and BV had no significant effects on the vitality of Leishmania promastigotes. However, they had a high mortality effect on macrophages. The highest mortality belonged to propolis (78.39 %). In vivo results showed significant differences between some treated and control groups in terms of the mean ulcer size.
Conclusion: It seems that a combination of honeybee venom and propolis in a particular dosage can prevent the development of the ulcers caused by L. major. More studies are needed to evaluate the effects of their constituent compounds precisely.
کلیدواژه‌ها
Leishmaniasis؛ Cutaneous؛ Bee venoms؛ Propolis؛ Wounds and injuries؛ Anti-ulcer agents
سایر فایل های مرتبط با مقاله
مراجع
  1. Alvar J, Cañavate C, Gutiérrez-Solar B, Jiménez M, Laguna F, López-Vélez R, et al. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clinical microbiology reviews. 1997;10(2):298-319. PMID: 9105756 PMCID: PMC172921 DOI: 10.1128/CMR.10.2.298.
  2. Desjeux P. Leishmaniasis: current situation and new perspectives. Comparative Immunology, Microbiology and Infectious Diseases. 2004;27(5):305-18. PMID: 15225981 DOI: 10.1016/j.cimid.2004.03.004.
  3. Bray RS. Leishmania. Annual Review of Microbiology. 1974;28(1):189-217. PMID: 4611328 DOI: 10.1146/annurev.mi.28.100174.001201.
  4. Azizi K, Kalantari M, Motazedian M, Asgari Q, Mohammadpour I, Soltani A. Co-detection and isolation of Leishmania and Crithidia among naturally infected Tatera indica (Rodentia: Muridae) in Fars province, southern Iran. Asian Pacific Journal of Tropical Biomedicine. 2018;8(5):279. DOI: 10.4103/2221-1691.233010.
  5. Nazari M, Taravatmanesh G, Kaveh MH, Soltani A, Ghaem H. The Effect of Educational Intervention on Preventive Behaviors towards Cutaneous Leishmaniasis at Kharameh City in 2014. Shiraz E-Medical Journal. 2016;17(10). DOI: 10.17795/semj39957.
  6. Rebouças-Silva J, Celes FS, Lima JB, Barud HS, de Oliveira CI, Berretta AA, et al. Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts. Evid Based Complement Alternat Med. 2017;2017:6067172. PMID: 28690662 PMCID: PMC5485350 DOI: 10.1155/2017/6067172.
  7. Pucca MB, Cerni FA, Oliveira IS, Jenkins TP, Argemí L, Sørensen CV, et al. Bee Updated: Current Knowledge on Bee Venom and Bee Envenoming Therapy. Front Immunol. 2019;10:2090. PMID: 31552038 PMCID: PMC6743376 DOI: 10.3389/fimmu.2019.02090.
  8. Wehbe R, Frangieh J, Rima M, El Obeid D, Sabatier J-M, Fajloun Z. Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests. Molecules. 2019;24(16):2997. PMID: 31430861 PMCID: PMC6720840 DOI: 10.3390/molecules24162997.
  9. Ram SKM, Jayapal N, Nanaiah P, Aswal GS, Ramnarayan B, Taher SM. The therapeutic benefits of bee venom. International Journal of Current Microbiology and Applied Sciences. 2014;3:377-81.
  10. An H-J, Kim J-Y, Kim W-H, Gwon M-G, Gu HM, Jeon MJ, et al. Therapeutic effects of bee venom and its major component, melittin, on atopic dermatitis in vivo and in vitro. Br J Pharmacol. 2018;175(23):4310-24. PMID: 30187459 PMCID: PMC6240132 DOI: 10.1111/bph.14487.
  11. Nina N, Lima B, Feresin GE, Giménez A, Salamanca Capusiri E, Schmeda‐Hirschmann G. Antibacterial and leishmanicidal activity of Bolivian propolis. Letters in applied microbiology. 2016;62(3):290-6. PMID: 26743801 DOI: 10.1111/lam.12543.
  12. Ayres DC, Marcucci MC, Giorgio S. Effects of Brazilian propolis on Leishmania amazonensis. Mem Inst Oswaldo Cruz. 2007;102(2):215-20. PMID: 17426888 DOI: 10.1590/s0074-02762007005000020.
  13. Machado GMdC, Leon LL, Castro SLd. Activity of Brazilian and Bulgarian propolis against different species of Leishmania. Mem Inst Oswaldo Cruz. 2007;102:73-7. PMID: 17294003 DOI: 10.1590/s0074-02762007000100012.
  14. da Silva SS, Mizokami SS, Fanti JR, Miranda MM, Kawakami NY, Teixeira FH, et al. Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB/c mice. Parasitology research. 2016;115(4):1557-66. PMID: 26711452 DOI: 10.1007/s00436-015-4890-4.
  15. Duran G, Duran N, Culha G, Ozcan B, Oztas H, Ozer B. In vitro antileishmanial activity of Adana propolis samples on Leishmania tropica: a preliminary study. Parasitology Research. 2008;102(6):1217-25. PMID: 18264819 DOI: 10.1007/s00436-008-0896-5.
  16. Fidalgo LM, Ramos IS, Parra MG, Cuesta-Rubio O, Hernández IM, Fernández MC, et al. Activity of Cuban propolis extracts on Leishmania amazonensis and Trichomonas vaginalis. Natural product communications. 2011;6(7):1934578X1100600712. PMID: 21834236.
  17. Ferreira FM, Castro RA, Batista MA, Rossi FM, Silveira-Lemos D, Frézard F, et al. Association of water extract of green propolis and liposomal meglumine antimoniate in the treatment of experimental visceral leishmaniasis. Parasitology research. 2014;113(2):533-43. PMID: 24292604 DOI: 10.1007/s00436-013-3685-8.
  18. Światły-Błaszkiewicz A, Mrówczyńska L, Matuszewska E, Lubawy J, Urbański A, Kokot ZJ, et al. The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study. Metabolites. 2020;10(5):191. PMID: 32413967 PMCID: PMC7281017 DOI: 10.3390/metabo10050191.
  19. Benton AW, Morse RA, Stewart JD. Venom Collection from Honey Bees. Science. 1963;142(3589):228-30. PMID: 17834840 DOI: 10.1126/science.142.3589.228.
  20. 10993-5: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. 2009: International Organisation for Standardization; 2009. 34 p.
  21. Asgari Q, Keshavarz H, Rezaeian M, Motazedian MH, Shojaee S, Mohebali M, et al. Direct Effect of Two Naphthalene-Sulfonyl-Indole Compounds on Toxoplasma gondii Tachyzoite. J Parasitol Res. 2013;2013:716976. PMID: 24228173 PMCID: PMC3818922 DOI: 10.1155/2013/716976.
  22. Maroufi Y, Ghaffarifar F, Dalimi A-A, Sharifi Z, Hassan Z. Cantharidin-induced apoptosis in Leishmania major promastigotes and macrophages infected by Leishmania major amastigotes in vitro. Journal of Mazandaran University of Medical Sciences. 2012;22(89):32-40.
  23. Escobar P, Matu S, Marques C, Croft SL. Sensitivities of Leishmania species to hexadecylphosphocholine (miltefosine), ET-18-OCH3 (edelfosine) and amphotericin B. Acta Tropica. 2002;81(2):151-7. PMID: 11801222 DOI: 10.1016/s0001-706x(01)00197-8.
  24. Chaisakul J, Hodgson WC, Kuruppu S, Prasongsook N. Effects of Animal Venoms and Toxins on Hallmarks of Cancer. J Cancer. 2016;7(11):1571-8. MID: 27471574 PMCID: PMC4964142 DOI: 10.7150/jca.15309.
  25. Kim Y-S, Jun H, Chae Y, Park H-J, Kim BH, Chang I-M, et al. The practice of Korean medicine: an overview of clinical trials in acupuncture. Evid Based Complement Alternat Med. 2005;2(3):325-52. PMID: 16136212 PMCID: PMC1193543 DOI: 10.1093/ecam/neh102.
  26. Kim Y-W, Chaturvedi PK, Chun SN, Lee YG, Ahn WS. Honeybee venom possesses anticancer and antiviral effects by differential inhibition of HPV E6 and E7 expression on cervical cancer cell line. Oncology Reports. 2015;33(4):1675-82. PMID: 25633640 DOI: 10.3892/or.2015.3760.
  27. Pereira AV, de Barros G, Pinto EG, Tempone AG, Orsi RdO, Dos Santos LD, et al. Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response. J Venom Anim Toxins Incl Trop Dis. 2016;22:1. PMID: 26752985 PMCID: PMC4706697 DOI: 10.1186/s40409-016-0055-x.
  28. Adade CM, Oliveira IRS, Pais JAR, Souto-Padrón T. Melittin peptide kills Trypanosoma cruzi parasites by inducing different cell death pathways. Toxicon. 2013;69:227-39. PMID: 23562368 DOI: 10.1016/j.toxicon.2013.03.011.
  29. Ali M. Studies on bee venom and its medical uses. International journal of advanced engineering research and technology. 2012;1(2):69-83.
  30. da Silva SS, Thomé GdS, Cataneo AHD, Miranda MM, Felipe I, Andrade CGTdJ, et al. Brazilian propolis antileishmanial and immunomodulatory effects. Evid Based Complement Alternat Med. 2013;2013:673058. PMID: 23762152 PMCID: PMC3670560 DOI: 10.1155/2013/673058.
  31. Król W, Bankova V, Sforcin JM, Szliszka E, Czuba Z, Kuropatnicki AK. Propolis: properties, application, and its potential. Evid Based Complement Alternat Med. 2013;2013:807578. PMID: 23997799 PMCID: PMC3753737 DOI: 10.1155/2013/807578.
  32. Wagh VD. Propolis: a wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013:308249. PMID: 24382957 PMCID: PMC3872021 DOI: 10.1155/2013/308249.
  33. Anjum SI, Ullah A, Khan KA, Attaullah M, Khan H, Ali H, et al. Composition and functional properties of propolis (bee glue): A review. Saudi J Biol Sci. 2019;26(7):1695-703. MID: 31762646 PMCID: PMC6864204 DOI: 10.1016/j.sjbs.2018.08.013.
  34. Al-Waili N, Hozzein WN, Badr G, Al-Ghamdi A, Al-Waili H, Al-Waili T, et al. Propolis and bee venom in diabetic wounds; a potential approach that warrants clinical investigation. African Journal of Traditional, Complementary and Alternative Medicines. 2015;12(6):1. DOI: 10.4314/ajtcam.v12i6.1
  35. Amin MA, Abdel-Raheem IT, Madkor HR. Wound healing and anti-inflammatory activities of bee venom-chitosan blend films. Journal of Drug Delivery Science and Technology. 2008;18(6):424-30. DOI: 10.1016/S1773-2247(08)50082-7
  36. Antonio LdF, Lyra MR, Saheki MN, Schubach AdO, Miranda LdFC, Madeira MdF, et al. Effect of secondary infection on epithelialisation and total healing of cutaneous leishmaniasis lesions. Mem Inst Oswaldo Cruz. 2017;112(9):640-6. PMID: 28902290 PMCID: PMC5572450 DOI: 10.1590/0074-02760160557.
آمار
تعداد مشاهده مقاله: 373
تعداد دریافت فایل اصل مقاله: 314


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب