|تعداد مشاهده مقاله||10,670,327|
|تعداد دریافت فایل اصل مقاله||5,133,265|
Evaluation of Innate Lymphoid Cells (ILCs) Population in the Mouse Model of Colorectal Cancer
|Iranian Journal of Immunology|
|دوره 19، شماره 4، اسفند 2022، صفحه 339-348 اصل مقاله (1.41 M)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.22034/iji.2022.92467.2152|
|Seyed Mohammad Javadzadeh 1؛ Mohsen Keykhosravi 1؛ Mohsen Tehrani 1، 2؛ Hossein Asgarian-Omran 1، 2، 3؛ Mohsen Rashidi 4؛ Hadi Hossein-Nattaj1؛ Laleh Vahedi-Larijani5؛ Abolghasem Ajami 1، 6، 7|
|1Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran|
|2Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran|
|3Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari, Iran|
|4Department of Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran|
|5Department of Pathology, Mazandaran University of Medical Sciences, Sari, Iran|
|6Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran|
|7Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran|
| Background: Innate Lymphoid Cells (ILCs) promote tissue homeostasis, contribute to the immune defense mechanisms, and play important roles in the initiation of immune responses and chronic inflammation.|
Objective: To understand the roles of innate lymphoid cells in the pathophysiology of colorectal cancer (CRC) in the mouse model.
Methods: CRC was induced using azoxymethane (AOM) and dextran sulfate sodium (DSS) in Balb/c mice (the chemically induced group=18 mice), or orthotopic injection of CT-26 cell line into the colon of another set of Balb/c mice (the orthotopic group=14mice). Normal saline was injected into 18 mice, as the sham group. After 80 days, the chemically induced group was divided into two subgroups, dysplasia (8 mice) and reparative change (10 mice), based on pathological examinations. The frequencies of ILC1, 2, and 3 were then measured in colon tissues using flow cytometry by four markers including an anti-mouse lineage cocktail (FITC anti-mCD3/FITC anti-mGr-1/FITC anti-mCD11b/ FITC anti-mCD45R (B220)/FITC anti-mTer-119), PE/Cy7 anti-mouse CD45, PE anti-mouse CD117 (c-kit), and APC anti-mouse IL-33 Rα (ST2).
Results: The total ILC population was significantly higher in the chemically induced reparative change compared with the sham group. ILC1 percentage in the chemically induced reparative change was significantly higher compared to those in the other three groups (Sham, chemically induced dysplasia and orthotopic dysplasia). The orthotopic dysplasia group showed more ILC3 percentage than the other groups.
Conclusion: ILC1 and ILC3 subgroups increased significantly in reparative and dysplastic experimental CRC respectively. Thus ILC1 may have an inhibitory effect on tumor growth whereas ILC3 promotes tumor progression.
|Colorectal Cancer؛ CT-26؛ Dysplasia؛ Innate Lymphoid Cell؛ Reparative Change|
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