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What Happens When Methamphetamine Is Added to Nutrients of Cell Culture Medium? In Vitro Assessment of Morphological, Growth and Differential Potential of Wharton’s Jelly Stem Cells
|International Journal of Nutrition Sciences|
|مقاله 7، دوره 7، شماره 4، اسفند 2022، صفحه 233-240 اصل مقاله (892.02 K)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.30476/ijns.2022.97432.1210|
|Narges Mohammdzadeh 1؛ Davood Mehrabani 2، 3؛ Shahrokh Zare3؛ Shima Sadat Masoumi4؛ Aghdass Rasouli-Nia5؛ Feridoun Karimi-Busheri5|
|1Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran|
|2Li Ka Shing Center for Health Research and Innovation, University of Alberta, Edmonton, AB, Canada|
|3Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran|
|4International Branch, Faculty of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran|
|5Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, Alberta, Canada|
|Background: Methamphetamine use and misuse cause severe side effects in different body organs and are associated with socioeconomic consequences. This study aimed to investigate the in vitro effect of methamphetamine on cell morphology, growth and differentiation potential in Wharton’s jelly stem cells (WJSCs) when added to the culture|
Methods: MTT assay was applied to assess the toxicity of recreational dose of 60 μg/mL of methamphetamine on WJSCs. The cells were characterized morphologically, by osteo- and adipo-genic differentiation potential and by flow cytometry. Population doubling time of stem cells was determined and after exposure to methamphetamine was compared
with the control.
Results: WJSCs were adherent to culture flasks, were spindle shape and positive for osteo- and adipo-genic inductions and expressed mesenchymal markers, while lacking hematopoietic markers. Methamphetamine resulted in a reduction in cell proliferation and viability.
Conclusion: Our results can be the first study evaluating the in vitro effect of methamphetamine on WJSCs at cellular level revealing a decrease in cell proliferation, and viability when cells were exposed and treated with methamphetamine. These findings can be added to the literature especially when methamphetamine is targeted for recreational purposes.
|Methamphetamine؛ Wharton’s jelly stem cells؛ Morphology؛ Growth؛ Differentiation|
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