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Khazaei, Fatemeh, Bozorgi, Maryam, Khazaei, Mozafar. (1401). Anticancer Effect of Carob Bean Extract on Human Prostate Cancer Cell Lines: Apoptosis Induction and Bax/Bcl-2 Ratio Improvement. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/mejc.2022.93823.1704
Fatemeh Khazaei; Maryam Bozorgi; Mozafar Khazaei. "Anticancer Effect of Carob Bean Extract on Human Prostate Cancer Cell Lines: Apoptosis Induction and Bax/Bcl-2 Ratio Improvement". سامانه مدیریت نشریات علمی, , , 1401, -. doi: 10.30476/mejc.2022.93823.1704
Khazaei, Fatemeh, Bozorgi, Maryam, Khazaei, Mozafar. (1401). 'Anticancer Effect of Carob Bean Extract on Human Prostate Cancer Cell Lines: Apoptosis Induction and Bax/Bcl-2 Ratio Improvement', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/mejc.2022.93823.1704
Khazaei, Fatemeh, Bozorgi, Maryam, Khazaei, Mozafar. Anticancer Effect of Carob Bean Extract on Human Prostate Cancer Cell Lines: Apoptosis Induction and Bax/Bcl-2 Ratio Improvement. سامانه مدیریت نشریات علمی, 1401; (): -. doi: 10.30476/mejc.2022.93823.1704

Anticancer Effect of Carob Bean Extract on Human Prostate Cancer Cell Lines: Apoptosis Induction and Bax/Bcl-2 Ratio Improvement

Middle East Journal of Cancer
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 04 دی 1401  XML اصل مقاله (928.1 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/mejc.2022.93823.1704
نویسندگان
Fatemeh Khazaeiorcid ؛ Maryam Bozorgiorcid ؛ Mozafar Khazaei email orcid
Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
چکیده
Background: Carob (Ceratonia siliqua L.) has been used to cure various diseases in traditional medicine. This plant has also exerted anti-proliferative effects on certain cancer types. The present study aimed to determine the effects of carob bean extracts on proliferation and apoptotic genes (Bax, Bcl-2, and P53) and Caspase-3, -8, and -9 expressions of human prostate cancer cell lines.
Method: In this in vitro experimental study, human prostate cell lines (LNCaP and PC3) were treated with carob bean extract (0, 50, 100, 200, 400, and 800 µg/ml) for 24, 48, 72, and 96 h. Cell viability was investigated via MTT assay. The genes expression: Bax (pro-apoptotic), Bcl-2 (anti-apoptotic), Caspase-3 (required enzyme for the execution of apoptosis), Caspase-8 (mediator of the extrinsic pathway), and Caspase-9 (mediator of the intrinsic pathway) genes as well as P53 (tumor suppressor) were assessed using real-time polymerase chain reaction. Moreover, the nitric oxide in culture media was evaluated.
Results: Carob bean extract suppressed the proliferation of prostate cancer cells in a time and dose-dependent manner by inducing intrinsic apoptotic pathways and decreasing nitric oxide production (P <0.01). The obtained results revealed the overexpression of Caspases-3 and -9, P53, and Bax, but reduction in Bcl-2 expression, giving rise to a higher Bax/Bcl-2 ratio (P <0.05).
Conclusion: The carob bean extracts exerted anti-prostate cancer properties via induction of apoptosis. It could be also suggested as a dietary supplement for patients suffering from prostate cancer.
کلیدواژه‌ها
Prostatic neoplasm؛ Apoptosis؛ Antineoplastic agents؛ Locust bean gum
مراجع
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