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Esmaeil Nejad, Azadeh, Talebi Ardakani, Mohammad Reza, Shokri, Mehdi, Hosseini Khou, Nima, Kamani, Mobina. (1402). Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study. سامانه مدیریت نشریات علمی, 24(2), 235-244. doi: 10.30476/dentjods.2022.93305.1709
Azadeh Esmaeil Nejad; Mohammad Reza Talebi Ardakani; Mehdi Shokri; Nima Hosseini Khou; Mobina Kamani. "Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study". سامانه مدیریت نشریات علمی, 24, 2, 1402, 235-244. doi: 10.30476/dentjods.2022.93305.1709
Esmaeil Nejad, Azadeh, Talebi Ardakani, Mohammad Reza, Shokri, Mehdi, Hosseini Khou, Nima, Kamani, Mobina. (1402). 'Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study', سامانه مدیریت نشریات علمی, 24(2), pp. 235-244. doi: 10.30476/dentjods.2022.93305.1709
Esmaeil Nejad, Azadeh, Talebi Ardakani, Mohammad Reza, Shokri, Mehdi, Hosseini Khou, Nima, Kamani, Mobina. Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study. سامانه مدیریت نشریات علمی, 1402; 24(2): 235-244. doi: 10.30476/dentjods.2022.93305.1709

Comparative Evaluation of the Effect of Two Platelet Concentrates (a-PRF and L-PRF) on the Cellular Activity of Pre-osteoblastic MG-63 Cell Line: An in vitro Study

Journal of Dentistry
مقاله 16، دوره 24، شماره 2 - شماره پیاپی 79، شهریور 2023، صفحه 235-244    اصل مقاله (559.51 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2022.93305.1709
نویسندگان
Azadeh Esmaeil Nejad1؛ Mohammad Reza Talebi Ardakani1؛ Mehdi Shokri2؛ Nima Hosseini Khou3؛ Mobina Kamani* 4
1Dept. of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2Dept. of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Private practice, Tehran, Iran.
4Postgraduate student, Dept. of Periodontics, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
چکیده
Statement of the Problem: Currently, the reconstruction of bone defects with new platelet concentrates is considered a significant challenge in periodontics.
Purpose: This study aimed to evaluate advanced- platelet rich fibrin (A-PRF) and leukocyte- and platelet rich fibrin’s (L-PRF) effects on the proliferation and differentiation of MG-63 cells.
Materials and Method: In this in vitro study, blood samples of five healthy non-smoking volunteers were collected and immediately centrifuged according to the two protocols of Choukroun and Ghanaati, without adding any anticoagulants, to prepare L-PRF and A-PRF. After freezing the clots for one hour, they were crushed and centrifuged once more. After culturing MG-63 cells, the effects of 20%, 10%, 1%, and 0.5% concentrations of A-PRF and L-PRF extracts on cell proliferation and mineralization were evaluated by methyl thiazolyl tetrazolium (MTT) assay and Alizarin Red staining, respectively.
Results: Generally, survival and proliferation in the L-PRF group at both time intervals were higher than the A-PRF group and increased with increasing the extract concentration. However, in the A-PRF group, there were no significant differences between the different concentrations, and only the number of cells increased over time. After three days, in the study on mineralization, nodule formation was observed only in the positive control group (osteogenic). In seven days, mineralized nodules were formed in all groups with different concentrations of A-PRF, but not in any of the L-PRF groups.
Conclusion: According to the results, L-PRF increased proliferation, and A-PRF exerted a positive effect on the differentiation of MG-63 cells.

تازه های تحقیق

Azadeh Esmaeilnejad Google Scholar

Mobina Kamani Google Scholar

کلیدواژه‌ها
Platelet-Rich Fibrin؛ Bone Regeneration؛ Cell Proliferation؛ Cell Differentiation
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