|تعداد مشاهده مقاله||10,670,297|
|تعداد دریافت فایل اصل مقاله||5,133,211|
Determination of Osteoblast Cell Viability and Histological Changes of Samples Obtained from Different Implant Drills during Osteotomy
|Journal of Dentistry|
|مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 26 آذر 1401 اصل مقاله (322.63 K)|
|نوع مقاله: Original Article|
|شناسه دیجیتال (DOI): 10.30476/dentjods.2022.91439.1579|
|Janet Moradi Haghgoo1؛ Masoumeh Khoshhal2؛ Shahram Sharifi2؛ iraj Khodadadi3؛ Hamidreza Ghadidmi pour4؛ Mohammad Ali Seif Rabie5؛ Nazli Rabienejad 1|
|1Dept. of Periodontics, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.|
|3Dept. of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|
|4Dept. of Pathology, Hamadan University of Medical Sciences, Hamadan, Iran.|
|5Dept. of Sociable Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.|
|atement of the Problem: The bone particles collected during osteotomy could be used as autogenous bone graft materials for dental implant surgery. Different factors such as drill design may influence its clinical viability.|
Purpose: This study examined the effect of drill design on the osteoblast viability and histopathology parameters of bone collected during the preparation of dental implant site.
Materials and Method: In this experimental study, 90 samples were obtained from three different bone drilling systems including Bego, Implantium, and Dio during fixture installation in patients requiring treatment at the Department of Periodontology, Dentistry University Hamedan. The MTT (3-4,5-Dimethylthiazol2,5-diphenyltetrazolium bromide) was used to determine percentage of cell viability. Samples were fixed in 10% formaldehyde for hist-ological evaluation. Then, they were kept in 10% EDTA solution for 4 weeks for decalcific-ation. The provided slides were evaluated regarding bone structure and osteocytes counts for assessment of viability. Tukey test and SPPS 21 software were used for statistical analysis.
Results: The result showed the viability of osteoblast obtained by Dio (0.45±0.04) was significantly better than Bego (0.37±0.05) and Implantium (0.37±0.04) systems. In histopathological evaluation, the grafting material obtained by Dio presented the best osteoblast morphology.
Conclusion: It may be concluded that drill geometry significantly influenced the viability of bone particles collected during the preparation of implant sites .Moreover, characteristic geometry alone cannot represent the performance of a particular drill, and several geometric features should be concerned. The results of this study showed that the geometry of the Dio drill was the best considering the viability and histopathological evaluations.
|Autogenous bone graft؛ Bone collection؛ Particle size؛ Drill geometry|
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