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Kadeh, Hamideh, Saravani, Shirin, Moradi, Mahsa, Alimanesh, Niloofar. (1401). A Comparative Evaluation of the Genotoxic Effects of Mobile Phone Radiation Using Buccal Micronucleus Assay. سامانه مدیریت نشریات علمی, 24(Supplement-March-2023), 118-124. doi: 10.30476/dentjods.2022.92515.1656
Hamideh Kadeh; Shirin Saravani; Mahsa Moradi; Niloofar Alimanesh. "A Comparative Evaluation of the Genotoxic Effects of Mobile Phone Radiation Using Buccal Micronucleus Assay". سامانه مدیریت نشریات علمی, 24, Supplement-March-2023, 1401, 118-124. doi: 10.30476/dentjods.2022.92515.1656
Kadeh, Hamideh, Saravani, Shirin, Moradi, Mahsa, Alimanesh, Niloofar. (1401). 'A Comparative Evaluation of the Genotoxic Effects of Mobile Phone Radiation Using Buccal Micronucleus Assay', سامانه مدیریت نشریات علمی, 24(Supplement-March-2023), pp. 118-124. doi: 10.30476/dentjods.2022.92515.1656
Kadeh, Hamideh, Saravani, Shirin, Moradi, Mahsa, Alimanesh, Niloofar. A Comparative Evaluation of the Genotoxic Effects of Mobile Phone Radiation Using Buccal Micronucleus Assay. سامانه مدیریت نشریات علمی, 1401; 24(Supplement-March-2023): 118-124. doi: 10.30476/dentjods.2022.92515.1656

A Comparative Evaluation of the Genotoxic Effects of Mobile Phone Radiation Using Buccal Micronucleus Assay

Journal of Dentistry
مقاله 6، دوره 24، Supplement-March-2023 - شماره پیاپی 1، خرداد 2023، صفحه 118-124    اصل مقاله (535.34 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2022.92515.1656
نویسندگان
Hamideh Kadeh1؛ Shirin Saravani* 1؛ Mahsa Moradi2؛ Niloofar Alimanesh3
1Oral & Dental Disease Research Center, Dept. of Oral & Maxillofacial Pathology, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
2Postgraduate Student, Dept. of Pediatric Dentistry, School of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
3Dentist, School of Dentistry, Zahedan University of Medical Science, Zahedan, Iran
چکیده
Statement of the Problem: Mobile usage has increased worldwide over the past two decades. There are conflicting reports about the carcinogenic effects of cell phone radiation on the oral mucosa. Micronucleus (MN) is considered a reliable marker for genotoxic damage.
Purpose: This study aimed to identify the impact of mobile phone radiation on the MN frequency in oral mucosal cells.
Materials and Method: In this descriptive-analytical study, 50 mobile phone users between the age group of 20–38 years were included. Samples were obtained from the right and left cheek mucosa of each subject (a total 100 cell samples). Every participant filled out a questionnaire about his or her cell phone usage habits. Additionally, personal information such as age, gender, and body mass index (BMI) were assessed. The Feulgen and Papanicolaou staining methods were used for staining of the cell samples. A total of 1000 cells in each sample were evaluated for MNs.
Results: The mean number of MN in exposed and non-exposed mucosa by Feulgen method was 0.71±1.13 and 0.57±1.36, respectively. Also in Papanicolaou staining, the mean number of MN in the exposed mucosa and non-exposed mucosa was 6.94±6.61 and 6.54±6.88, respectively, but these differences were not significant (p> 0.05). The frequency of MN in non-specific DNA staining was significantly (5- to 6-fold) higher than DNA-specific staining. We observed no statically significant differences between MN frequency according to age, gender, BMI, and other cell phone usage habits (p> 0.05).
Conclusion: This study showed that cell phone use does not cause genotoxic effects in the buccal mucosa in the oral cavity. Moreover, using non-specific DNA staining methods can increase the frequency of MN by more than 5- to 6-fold.
کلیدواژه‌ها
Cell Phone؛ Buccal Mucosa؛ Micronuclei؛ Genotoxicity
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