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Zhu, Chaohui, Fan, Min, Zhu, Jianhua, Cao, Limin, Duan, Xinyu, Wu, Kai. (1401). Vitamin D Reduces the Helper T Cells 17 (Th17) Differentiation in Patients with Ulcerative Colitis by Targeting Long Non-coding RNA (lncRNA) OIP5-AS1/miR-26a-5p/IL-6 Axis. سامانه مدیریت نشریات علمی, 19(2), 150-160. doi: 10.22034/iji.2022.90562.2014
Chaohui Zhu; Min Fan; Jianhua Zhu; Limin Cao; Xinyu Duan; Kai Wu. "Vitamin D Reduces the Helper T Cells 17 (Th17) Differentiation in Patients with Ulcerative Colitis by Targeting Long Non-coding RNA (lncRNA) OIP5-AS1/miR-26a-5p/IL-6 Axis". سامانه مدیریت نشریات علمی, 19, 2, 1401, 150-160. doi: 10.22034/iji.2022.90562.2014
Zhu, Chaohui, Fan, Min, Zhu, Jianhua, Cao, Limin, Duan, Xinyu, Wu, Kai. (1401). 'Vitamin D Reduces the Helper T Cells 17 (Th17) Differentiation in Patients with Ulcerative Colitis by Targeting Long Non-coding RNA (lncRNA) OIP5-AS1/miR-26a-5p/IL-6 Axis', سامانه مدیریت نشریات علمی, 19(2), pp. 150-160. doi: 10.22034/iji.2022.90562.2014
Zhu, Chaohui, Fan, Min, Zhu, Jianhua, Cao, Limin, Duan, Xinyu, Wu, Kai. Vitamin D Reduces the Helper T Cells 17 (Th17) Differentiation in Patients with Ulcerative Colitis by Targeting Long Non-coding RNA (lncRNA) OIP5-AS1/miR-26a-5p/IL-6 Axis. سامانه مدیریت نشریات علمی, 1401; 19(2): 150-160. doi: 10.22034/iji.2022.90562.2014

Vitamin D Reduces the Helper T Cells 17 (Th17) Differentiation in Patients with Ulcerative Colitis by Targeting Long Non-coding RNA (lncRNA) OIP5-AS1/miR-26a-5p/IL-6 Axis

Iranian Journal of Immunology
مقاله 3، دوره 19، شماره 2، شهریور 2022، صفحه 150-160    اصل مقاله (1.6 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22034/iji.2022.90562.2014
نویسندگان
Chaohui Zhu1؛ Min Fan2؛ Jianhua Zhu1؛ Limin Cao3؛ Xinyu Duan1؛ Kai Wu* 1
1Department of Gastroenterology, Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China
2Department of Gynaecology and Obstetrics, Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China
3College of Life Sciences, Capital Normal University, Beijing 100081, China
چکیده
Background: Vitamin D has anti-inflammatory efficacy against ulcerative colitis (UC), however, the mechanism is yet little understood. Objective: To investigate the immunomodulatory effects of vitamin D against the UC, and to explore the potential downstream mechanisms. Materials and methods: Serum vitamin D, Interferon-γ (IFN-γ) and Interleukin (IL)-17 levels of the patients with UC were quantified using enzyme-linked immunosorbent assay (ELISA). Long non-coding RNAs (lncRNAs) levels were determined by using quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Peripheral blood mononuclear cells (PBMCs) were collected from healthy control subjects, stimulated with CD4+ T lymphocytes or helper T cells 17(Th17) differentiation conditions, and then exposed to calcitriol (vitamin D active form) or certain lentiviral treatment, followed by subsequent molecular level testing. For in vivo assay, mice were given 3% dextran sulfate sodium (DSS) to induce colitis. Results: Compared with the control group, vitamin D levels in the UCs were statistically lower, and there was a negative correlation between IL-17 and vitamin D in the UCs. The lncRNA OIP5-AS1 could decrease under calcitriol treatment in both CD4+ T cells and Th17 differentiation. The lncRNA OIP5-AS1 was a microRNA (miR)-26a-5p sponge and therefore modulated the Th17 cells and IL-6 expression. The lncRNA OIP5-AS1/miR-26a-5p/IL-6 axis mediated the regulation of calcitriol-induced Th17 differentiation. Calcitriol had therapeutic effects on the UC mouse models by regulating the lncRNA OIP5-AS1 related pathway. Conclusion: Vitamin D might have anti-inflammatory potential in the treatment of the UC.
کلیدواژه‌ها
lncRNA؛ Th17 Differentiation؛ Ulcerative Colitis؛ Vitamin D
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